Soluble components from mesenchymal stromal cell processing exert anti-inflammatory effects and facilitate ischemic muscle regeneration

Huang, Wenbai; Kraynak, Chelsea A.; Bender, Elizabeth C.; Farrar, Roger P.; Suggs, Laura J.

doi:10.1016/j.jcyt.2022.11.010

Cited by 2 publications

(7 citation statements)

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“…Macrophages were isolated from mouse bone marrow with a method adapted from Huang et al 31 In brief, the femur and tibia bones were isolated from the hind legs of a C57BL/6 female mouse at the age of 8–12 weeks. Bones were temporarily soaked in phosphate‐buffered saline (PBS) on ice and clipped at both ends to preserve cell viability and reduce metabolic activities.…”

Section: Methodsmentioning

confidence: 99%

“…Macrophage activation studies were performed as previously described 31 . Briefly, macrophages were seeded on 12‐well plates at 125,000 cells/well.…”

Section: Methodsmentioning

confidence: 99%

“…For each passage, approximately 250,000-500,000 cells were seeded into a T25 flask for expansion. MSC-derived bioactive factors were isolated from MSCs (P2-P4) after freeze and thaw (F&T)-induced apoptosis, as described by Huang et al 31 Two million MSCs were lifted and suspended in 1 mL RPMI supplemented with reduced HI-FBS content (1% HI-FBS). The cell suspension was transferred to a cryovial and sealed by parafilm.…”

Section: Msc Harvest and Isolation Of Msc-derived Bioactive Factorsmentioning

confidence: 99%

“…Recent studies have shown that apoptotic‐like MSCs exerted immunoregulatory effects by releasing phosphatidylserine (PtS) or other components in the MSC secretome and activating the caspase cascade 29,30 . Huang et al reported that soluble components derived from apoptotic MSCs via freeze‐thawing cycles demonstrated immunoregulatory and angiogenic effects on macrophages 31 . The MSC supernatant was applied to treat ischemic muscle injury in an animal model and outperformed treatment by direct cell delivery in improving blood flow, inflammation resolution, and muscle function recovery.…”

Section: Introductionmentioning

confidence: 99%

“…29,30 Huang et al reported that soluble components derived from apoptotic MSCs via freeze-thawing cycles demonstrated immunoregulatory and angiogenic effects on macrophages. 31 The MSC supernatant was applied to treat ischemic muscle injury in an animal model and outperformed treatment by direct cell delivery in improving blood flow, inflammation resolution, and muscle function recovery. This study showed that the angiogenic and immunomodulatory effects of the MSC F&T supernatant significantly relied on the presence of PtS and identified three relevant soluble proteins, PAI-1, M-CSF, and IL-33, that could promote macrophage recruitment, proliferation, and polarization.…”

mentioning

confidence: 99%

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Hydrogel foam dressings with angiogenic and immunomodulatory factors from mesenchymal stem cells

Lan,

Fletcher,

Bender

et al. 2024

J Biomedical Materials Res

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Stem cell therapy and skin substitutes address the stalled healing of chronic wounds in order to promote wound closure; however, the high cost and regulatory hurdles of these treatments limit patient access. A low‐cost method to induce bioactive healing has the potential to substantially improve patient care and prevent wound‐induced limb loss. A previous study reported that bioactive factors derived from apoptotic‐like mesenchymal stem cells (MSCs) demonstrated anti‐inflammatory and proangiogenic effects and improved ischemic muscle regeneration. In this work, these MSC‐derived bioactive factors were loaded into a hydrogel foam to harness immunomodulatory and angiogenic properties from MSC components to facilitate chronic wound healing without the high cost and translational challenges of cell therapies. After incorporation of bioactive factors, the hydrogel foam retained high absorbency, moisture retention, and target water vapor transmission rate. High loading efficiency was confirmed and release studies indicated that over 90% of loaded factors were released within 24 h. Ethylene oxide sterilization and 4‐week storage did not affect the bioactive factor release profile or physical properties of the hydrogel foam dressing. Bioactivity retention of the released factors was also confirmed for as‐sterilized, 4°C‐stored, and −20°C‐stored bioactive hydrogel foams as determined by relevant gene expression levels in treated pro‐inflammatory (M1) macrophages. These results support the use of the bioactive dressings as an off‐the‐shelf product. Overall, this work reports a new method to achieve a first‐line wound dressing with the potential to reduce persistent inflammation and promote angiogenesis in chronic wounds.

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Section: Methodsmentioning

confidence: 99%

“…Macrophage activation studies were performed as previously described 31 . Briefly, macrophages were seeded on 12‐well plates at 125,000 cells/well.…”

Section: Methodsmentioning

confidence: 99%

Section: Msc Harvest and Isolation Of Msc-derived Bioactive Factorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Hydrogel foam dressings with angiogenic and immunomodulatory factors from mesenchymal stem cells

Lan,

Fletcher,

Bender

et al. 2024

J Biomedical Materials Res

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The effects of exercise on microRNA expression profiling in adipose tissue macrophages of mice

Qin,

Huang,

et al. 2024

Front. Immunol.

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BackgroundExercise is recognized for its broad health benefits, influencing various physiological processes, including the behavior of adipose tissue macrophages (ATMs). While existing studies mainly associate ATM activity with obesity and metabolic syndrome, our study explores the impact of aerobic exercise on ATM microRNA expression profiling in a non-obese context, highlighting its general health-promoting mechanisms.MethodsSixty male C57BL/6 mice were randomly assigned to either a sedentary (S) or an exercise (E) group. The S group remained inactive, while the E group underwent a one-week treadmill adaptation, followed by an 8-week aerobic treadmill exercise protocol (60 min/day, 5 days/week, at 65%-75% VO2max). Post-training, glucose tolerance and the serum lipid levels were measured in mice subjected to both exercise and non-exercise conditions. ATMs harvested from visceral adipose tissues were analyzed and sorted using flow cytometer. To further investigate the effects of exercise in ATMs at the molecular level, miRNA microarray analysis was performed, followed by bioinformatic analysis.ResultsThe 8-week regimen of moderate-intensity aerobic exercise ameliorated glucolipid metabolism and fostered a dynamic shift toward an M2 macrophage phenotype in the adipose tissue, independent of obesity. A total of 62 differentially expressed miRNAs were identified in ATMs of mice post-exercise. Notably, six miRNAs (miR-212-5p, miR-511-5p, miR-7b-5p, miR-142-3p, miR-1894-3p, and miR-31-5p) as well as their target gene were consistently altered and associated with macrophage polarization and metabolic regulation.ConclusionOur findings broaden the understanding of how exercise regulates ATM functions through significant changes in microRNA profiles, emphasizing its potential to enhance health and prevent chronic conditions. This study supports the application of aerobic exercise for its preventive effects on chronic diseases and underscores the importance of microRNA profiling in understanding the immune-modulatory impacts of exercise.

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Soluble components from mesenchymal stromal cell processing exert anti-inflammatory effects and facilitate ischemic muscle regeneration

Cited by 2 publications

References 44 publications

Hydrogel foam dressings with angiogenic and immunomodulatory factors from mesenchymal stem cells

Hydrogel foam dressings with angiogenic and immunomodulatory factors from mesenchymal stem cells

The effects of exercise on microRNA expression profiling in adipose tissue macrophages of mice

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