Soluble–insoluble enzyme catalysts

Charles, Marvin; Coughlin, Robert W.; Hasselberger, Francis X.

doi:10.1002/bit.260161113

Cited by 40 publications

(7 citation statements)

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“…The authors synthesized a hybrid subtilisin the solubility of which can be regulated by photoirradiation through coupling with a photoresponsive polymer. Although some methods of chemical modification for regulating enzyme solubility in aqueous media have been already reported, such as attachment of pH-, ionic strength-, temperature-, or redox-sensitive 102 polymers, that was the first approach acting in organic solvents. The polymer characteristically contains photoresponsive spiropyran groups that allow direct manipulation of the solubility of the modified subtilisin as the nonpolar spiropyran group is reversibly converted to the polar merocyanine form by UV and visible light irradiations (Scheme ).…”

Section: Improving Proteases For Synthesis In Organic Solventsmentioning

confidence: 99%

Proteases in Organic Synthesis

2002

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Section: Improving Proteases For Synthesis In Organic Solventsmentioning

confidence: 99%

Proteases in Organic Synthesis

2002

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“…This process is reversible. Such stimuli-responsive polymers might be applied in the medical or biotechnological fields for drug delivery systems [8][9][10] immuno-assays [11][12][13][14][15], and bioseparations [16][17][18][19][20][21]. For these reasons, many researchers are actively studying LCST polymers [8-15, 22-26, 33].…”

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confidence: 99%

Temperature-dependent adsorption/desorption behavior of lower critical solution temperature (LCST) polymers on various substrates

Miura¹,

Cole²,

Monji³

et al. 1994

Journal of Biomaterials Science, Polymer Edition

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We have been studying adsorption and retention (resistance to desorption) behavior of temperature sensitive LCST polymers on different substrates as a function of temperature. According to our studies with Poly 64 (a copolymer of 60% (mol) NIPAAm and 40% (mol) NnBAAm, LCST = 8.5 degrees C in water), the copolymer retention depends on the rinse temperature. When the rinse temperature is above the LCST, the polymer adheres well to most surfaces. On the contrary, at rinse temperatures below the LCST, most of the adsorbed polymer is easily rinsed off. These studies are relevant to our work on the thermally reversible adsorption of LCST polymers conjugated to peptides and proteins, such as affinity ligands, for uses in immunoassays and affinity separations. The interaction between the LCST polymer and most hydrophobic polymer surfaces is mainly due to hydrophobic interactions, and the critical surface tension (gamma c) and the solubility parameter (delta) of the solid polymer substrate are the most important factors which influence the LCST polymer adsorption and retention. The critical surface tension appears to correlate best with the LCST polymer adsorption levels on different substrates, while the solubility parameter correlates best with the retention of the adsorbed polymer. According to our preliminary study, n-butyl groups probably interact more strongly with the substrates than isopropyl groups because of the greater hydrophobic surface area of the former groups.

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“…The first such design was in fact reported with alginic acid (Charles et al, 1974). The polymer was linked to lysozyme.…”

Section: Applicationsmentioning

confidence: 98%