Solution properties and vesicle formation of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa SP4

Pornsunthorntawee, Orathai; Chavadej, Sumaeth; Rujiravanit, Ratana

doi:10.1016/j.colsurfb.2009.03.006

Cited by 89 publications

(47 citation statements)

References 40 publications

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“…Furthermore, the presence of larger fatty acids chains plays a role in decreasing the CMC of rhamnolipid. For instance, mono-rhamnolipids are less soluble, adsorb onto surfaces more strongly and have a higher CMC value for hydrocarbon solubilization [27]. In this study, the two fatty acids-nine rhamnolipid congeners produced by P. aeruginosa UKMP14T were expected to have high surface active activity, thus increasing the efficacy of biosurfactant.…”

Section: Moietymentioning

confidence: 89%

SPECTROSCOPIC ANALYSIS OF RHAMNOLIPID PRODUCED BY PRODUCED BY Pseudomonas aeruginosa UKMP14T

Sabturani¹,

Latif²,

Radiman³

et al. 2016

MJAS

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Biosurfactant produced by Pseudomonas aeruginosa UKMP14T was optimized by growing the isolate in mineral salt medium (MSM) supplemented with 1% (v/v) glycerol and 1.3 g/L ammonium sulphate with C/N ratio of 14:1. The culture medium was incubated at 37°C, with an agitation speed of 150 rpm for 7 days. P. aeruginosa UKMP14T produced biosurfactant at 0.8 g/L after 7 days incubation. Anthrone assay proved biosurfactant was glycolipid. The biosurfactant was characterized by scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDX) in addition to Fourier transform infra-red (FT-IR), nuclear magnetic resonance (NMR) and electrospray ionization-mass spectrometry (ESI-MS). The SEM-EDX analysis indicated the presence of carbon and oxygen elements by 78% and 22% (atomic %), respectively. FT-IR absorption spectra indicated the functional groups of rhamnolipid were located at 3308. 46, 2922.91, 2857.09 and 1730.96 cm -1 . ESI-MS/MS analyses identified P. aeruginosa UKMP14T produced rhamnolipid with two fatty acids-nine congeners, L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (Rha-Rha-C 10 -C 10 ) (m/z 649) which formed the main compound. The critical micelle concentration (CMC) of this rhamnolipid was established at 30 mg/L (32 dynes/cm). The characteristics of biosurfactant produced by P. aeruginosa UKMP14T indicated it has a high potential in industrial dan bioremediation application. Keywords: biosurfactant, Pseudomonas aeruginosa, rhamnolipid, spectroscopic analysis, CMC value AbstrakBiosurfaktan dihasilkan oleh Pseudomonas aeruginosa UKMP14T dioptimumkan dalam medium garam mineral (MSM) yang ditambahkan dengan 1% (i/i) gliserol dan 1.3 g/L ammonium sulfat dengan nisbah C/N iaitu 14:1. Medium kultur ini dieram pada 37°C, dengan kelajuan goncangan pada 150 psm selama 7 hari. P. aeruginosa UKMP14T menghasilkan biosurfaktan sebanyak 0.8 g/L selepas 7 hari pengeraman. Asai antron membuktikan biosurfaktan terhasil adalah glikolipid. Biosurfaktan tersebut dicirikan oleh mikroskop pengimbasan elektron-spektroskopi tenaga serakan sinaran-X (SEM-EDX), dengan penambahan Infra-merah transformasi Fourier (FT-IR), resonan magnetik nuklear (NMR) daknn spektrometri jisim-pengionan elektrosemburan (ESI-MS). Analisis SEM-EDX menunjukkan kehadiran unsur karbon dan oksigen masing-masing sebanyak 78% dan 22% (% atomik). Spektrum penyerapan FT-IR menunjukkan kumpulan berfungsi ramnolipid pada 3308.46, 2922.91, 2857.09 dan 1730.96 cm -1 . Analisis ESI-MS/MS mengenalpasti P. aeruginosa UKMP14T menghasilkan ramnolipid dengan sembilan kongener-dua asid lemak, L-ramnosil-L-ramnosil-β-hidroksidekanoil-β-hidroksidekanoat (Rha-Rha-C 10 -C 10 ) (m/z 649) sebagai sebatian utama. Kepekatan misel kritikal (CMC) ramnolipid ini dikesan pada 30 mg/L (32 dynes/cm). Ciri-ciri biosurfaktan yang dihasilkan oleh P. aeruginosa UKMP14T menunjukkan ia mempunyai potensi tinggi untuk di aplikasi dalam industri dan bioremediasi.

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Section: Moietymentioning

confidence: 89%

SPECTROSCOPIC ANALYSIS OF RHAMNOLIPID PRODUCED BY PRODUCED BY Pseudomonas aeruginosa UKMP14T

Sabturani¹,

Latif²,

Radiman³

et al. 2016

MJAS

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“…10,14,16,17,20 Thus, a need still exists to fully characterize the aggregation properties of these surfactants in solution as well as to probe the chemical microenvironments within these aggregates, especially at concentrations below ~20 mM. Toward this end, we report the combined use of surface tensiometry, fluorescence spectroscopy, 21,22 fluorescence quenching, 23,24 and DLS combined with all-atom molecular dynamics (MD) simulations to better understand the aggregation properties of anionic monorhamnolipids in aqueous solution at basic pH.…”

Section: Introductionmentioning

confidence: 99%

Evolution of Aggregate Structure in Solutions of Anionic Monorhamnolipids: Experimental and Computational Results

et al. 2017

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The evolution of solution aggregates of the anionic form of the native monorhamnolipid (mRL) mixture produced by Pseudomonas aeruginosa ATCC 9027 is explored at pH 8.0 using both experimental and computational approaches. Experiments utilizing surface tension measurements, dynamic light scattering, and both steady-state and time-resolved fluorescence spectroscopy reveal solution aggregation properties. All-atom molecular dynamics simulations on self-assemblies of the most abundant monorhamnolipid molecule, L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (Rha-C10-C10), in its anionic state explore the formation of aggregates and the role of hydrogen bonding, substantiating the experimental results. At pH 8.0, at concentrations above the critical aggregation concentration of 201 μM but below ~7.5 mM, small premicelles exist in solution; above ~7.5 mM, micelles with hydrodynamic radii of ~2.5 nm dominate, although two discrete populations of larger lamellar aggregates (hydrodynamic radii of ~10 and 90 nm) are also present in solution in much smaller number densities. The critical aggregation number for the micelles is determined to be ~26 monomers/micelle using fluorescence quenching measurements, with micelles gradually increasing in size with monorhamnolipid concentration. Molecular dynamics simulations on systems with between 10 and 100 molecules of Rha-C10-C10 indicate the presence of stable premicelles of seven monomers with the most prevalent micelle being ~25 monomers and relatively spherical. A range of slightly larger micelles of comparable stability can also exist that become increasing elliptical with increasing monomer number. Intermolecular hydrogen bonding is shown to play a significant role in stabilization of these aggregates. In total, the computational results are in excellent agreement with the experimental results.

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“…The research on the adsorbtion of rhamnolipids on bacterial cells performed by Zhong et al, (2008) lead to a conclusion, that the drop in cell surface hydrophobocity may be caused by a formation of multilayers or hemi-micelle accumulation. The increased substrate uptake through surfactant solubilisation may also be of importance, as reported by Pornsunthorntawee et al, (2009b). Rhamnolipid supplementation could result to a notably enhanced mineralization of hydrophobic substrates (Zhang et al, 2009).…”

Section: Resultsmentioning

confidence: 79%

Differences and dynamic changes in the cell surface properties of three Pseudomonas aeruginosa strains isolated from petroleum-polluted soil as a response to various carbon sources and the external addition of rhamnolipids

Górna

Ławniczak

Zgoła‐Grześkowiak

et al. 2011

Bioresource Technology

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Solution properties and vesicle formation of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa SP4

Cited by 89 publications

References 40 publications

SPECTROSCOPIC ANALYSIS OF RHAMNOLIPID PRODUCED BY PRODUCED BY Pseudomonas aeruginosa UKMP14T

SPECTROSCOPIC ANALYSIS OF RHAMNOLIPID PRODUCED BY PRODUCED BY Pseudomonas aeruginosa UKMP14T

Evolution of Aggregate Structure in Solutions of Anionic Monorhamnolipids: Experimental and Computational Results

Differences and dynamic changes in the cell surface properties of three Pseudomonas aeruginosa strains isolated from petroleum-polluted soil as a response to various carbon sources and the external addition of rhamnolipids

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