Solvent and stationary phase selectivity in capillary electrochromatography method development: Comparison of C18, C8, and phenyl-bonded phases for the separation of a series of substituted barbiturates

Euerby, Melvin R.; Johnson, Christopher M.; Smyth, Simon F.; Gillott, Nicola; Barrett, David A.; Shaw, P. Nicholas

doi:10.1002/(sici)1520-667x(1999)11:4<305::aid-mcs8>3.0.co;2-g

Cited by 35 publications

(3 citation statements)

References 12 publications

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“…The concentrations of citrate buffer were changed from 2 to 10 mM. In contrast to previous studies [23][24][25], the EOF velocity was not significantly affected by an increase in buffer concentration (EOF changed from 1.19 to 1.13 mm/s, when ncreasing the concentration from 2 to 10 mM).…”

Section: Effect Of Buffer Concentrationmentioning

confidence: 58%

Capillary electrochromatography of caffeine and its metabolites in rat brain microdialysate

et al. 2005

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A capillary electrochromatography (CEC) method has been developed for the separation of caffeine and its two metabolites 1-methylxanthine (1-MX) and 1,7-dimethylxanthine (1,7-DX). The stationary phase was 3-(1,8-naphthalimido) propyl-modified silyl silica gel (NAIP) and the best separations were achieved with 4.0 mM citrate buffer (pH 5.0) containing 80% methanol at an applied voltage of 25 kV. The compounds were completely separated in less than 3.5 min with good repeatability, which was approximately 3-times less than that in high-performance liquid chromatography (HPLC) with NAIP. The proposed method coupled with microdialysis was successfully applied to the monitoring of caffeine concentration in rat brain with detection limits of 1.11 microg/mL.

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Section: Effect Of Buffer Concentrationmentioning

confidence: 58%

Capillary electrochromatography of caffeine and its metabolites in rat brain microdialysate

et al. 2005

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“…These columns differ substantially in their hydrophobicity and silanol activity [24]. Both columns were conditioned by the mobile phase using a syringe pump and then in the CEC mode by applying 1 MPa pressure on both sides of the column and increasing the voltage from 0 -25 kV in 5 kV steps per 10 min.…”

Section: Columnsmentioning

confidence: 99%

Separation of basic central nervous system drugs by capillary electrochromatography

Jiskra

Claessens²,

Cramers

2003

J of Separation Science

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Separation of basic central nervous system drugs by capillary electrochromatographyThis article describes the sample preparation and separation of three strongly basic central nervous system (CNS) drugs by capillary electrochromatography. The separation was developed on Hypersil C8 MOS and Hypersil Phenyl stationary phases using acetonitrile (ACN) as organic modifier together with mobile phase additives, ammonia, ethylenediamine, or 1,3-diaminopropane. A successful, fast separation after a simple derivatization procedure of the compound was achieved on the Hypersil Phenyl stationary phase using ethylenediamine as mobile phase additive. The general approach to method development for complex mixtures is discussed.

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“…The packing materials used in CEC are generally derived from liquid chromatography (LC) technology [10]. Cs and phenyl [14], strong cation-exchange [15 17], strong anion-exchange [18], mixed mode [14,19], and chiral phases [20,21], has been investigated. Cs and phenyl [14], strong cation-exchange [15 17], strong anion-exchange [18], mixed mode [14,19], and chiral phases [20,21], has been investigated.…”

Section: Introductionmentioning

confidence: 99%

Separation of phenylureas by capillary electrochromatography on porous graphitic carbon

2003

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In this work the potential of porous graphitic carbon (PGC) as a new stationary phase in capillary electrochromatography has been explored. Its behavior under the action of an applied potential is described for the separation of phenylureas. First, it was shown that porous graphitic carbon enables high efficiency in capillary electrochromatography over a wide range of mobile phase velocities. It was then demonstrated that this material might be responsible for degradation of the solutes at frit-PGC interfaces. Although electrolytic degradation reactions are suspected to occur on this type of conductive material, voltamperometric measurements furnished no clear evidence.A specific injection procedure is proposed for avoiding degradation of the solutes at the inlet interface before their chromatographic separation. Comparison of the retention behavior of phenylureas on PGC in liquid chromatography and in capillary electrochromatography show that the retention properties of PGC are altered by application of an electrical field, because this modifies the donor-acceptor interactions bel',,veen the solutes and the stationary phase.Original

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Solvent and stationary phase selectivity in capillary electrochromatography method development: Comparison of C18, C8, and phenyl-bonded phases for the separation of a series of substituted barbiturates

Cited by 35 publications

References 12 publications

Capillary electrochromatography of caffeine and its metabolites in rat brain microdialysate

Capillary electrochromatography of caffeine and its metabolites in rat brain microdialysate

Separation of basic central nervous system drugs by capillary electrochromatography

Separation of phenylureas by capillary electrochromatography on porous graphitic carbon

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