Solvent Bar Microextraction with HPLC for Determination and Protein-Binding Characteristics of Oleanolic Acid and Ursolic Acid

Hao, Yaomei; Chen, Xuan; Hu, Shuang; Bai, Xiaohong

doi:10.1007/s10337-013-2599-z

Cited by 6 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the trace analysis of target analytes, sample preparation, which includes both clean-up and preconcentration before chromatographic analysis, is indispensable for many types of samples. Several sample pretreatment methods such as ultrasound-assisted extraction (UAE) [ 7 ], microwave-assisted extraction (MAE) [ 8 ], accelerated solvent extraction (ASE) [ 9 ], solvent bar microextraction (SBME) [ 10 ], and solid-phase extraction (SPE) [ 11 ] have been reported for the extraction and enrichment of UA and OA from different matrices prior to analysis by gas chromatography (GC) or liquid chromatography (LC). Among these methods, SPE is increasingly being used as a sample preparation technique because of its advantages of reducing sample handling, labour, and organic solvent consumption.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis of Ursolic Acid and Oleanolic Acid in Guava Leaves Using QuEChERS-Based Extraction Followed by High-Performance Liquid Chromatography

Liao

Fang

et al. 2017

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

In this paper, a novel method of QuEChERS-based extraction coupled with high-performance liquid chromatography has been developed for the simultaneous determination of ursolic acid (UA) and oleanolic acid (OA) in guava leaves. The QuEChERS-based extraction parameters, including the amount of added salt, vortex-assisted extraction time, and absorbent amount, and the chromatographic conditions were investigated for the analysis of UA and OA in guava leaves. Under the optimized conditions, the method showed good linearity over a range of 1–320 μg mL−1, with correlation coefficients above 0.999. The limits of detection of UA and OA were 0.18 and 0.36 μg mL−1, respectively. The intraday and interday precision were below 1.95 and 2.55%, respectively. The accuracies of the UA and OA determinations ranged from 97.4 to 111.4%. The contents of UA and OA in the guava leaf samples were 2.50 and 0.73 mg g−1, respectively. These results demonstrate that the developed method is applicable to the simultaneous determination of UA and OA in guava leaves.

show abstract

Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis of Ursolic Acid and Oleanolic Acid in Guava Leaves Using QuEChERS-Based Extraction Followed by High-Performance Liquid Chromatography

Liao

Fang

et al. 2017

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

show abstract

“…The simultaneous determination of triterpene acids using chromatographic and spectroscopic methods has been thoroughly studied over recent years. − Many extraction techniques to recover triterpene acids (Soxhlet, heat reflux, ultrasonic, and microwave) have been described from diverse plant tissues such as herbs, fruits, olive leaves, and olive-mill wastes . Romero et al extracted oleanolic acid and maslinic acid from table olives, using an exhaustive solid–liquid extraction technique with a mixture of methanol:ethanol (1:1), and Guinda et al also suggested ethanol as an appropriate solvent for extraction.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Solvent and the Sample Preparation on the Determination of Triterpene Compounds in Two-Phase Olive-Mill-Waste Samples

Fernández‐Hernández

Martı́nez

Rivas

et al. 2015

J. Agric. Food Chem.

View full text Add to dashboard Cite

A simple and rapid extraction method has been employed to determine several value-added compounds, mainly triterpenes, in two-phase olive-mill-waste samples. The compounds were extracted with methanol or ethyl acetate, and the initial fresh samples were treated for classic techniques such as drying, drying and oil extraction, and drying and sifting of the olive stones. For the identification and quantitation of the compounds, an ultra performance liquid chromatography-mass spectrometry method was employed. The best results of the triterpenic compound content were achieved by extraction with methanol from the fresh sample for the oleanolic and ursolic acids, and erythrodiol and uvaol; and from the dried-extracted sample for the maslinic acid. Conversely, the best results for the linoleic acid content were reached by extraction with ethyl acetate from the dried-sifted sample. These are remarkable processes that make the solid wastes from the olive-oil industry reach a high added value.

show abstract

Recent advances in the determination of unbound concentration and plasma protein binding of drugs: Analytical methods

Seyfinejad

Özkan

Jouyban

2021

Talanta

View full text Add to dashboard Cite

Solvent Bar Microextraction with HPLC for Determination and Protein-Binding Characteristics of Oleanolic Acid and Ursolic Acid

Cited by 6 publications

References 11 publications

Simultaneous Analysis of Ursolic Acid and Oleanolic Acid in Guava Leaves Using QuEChERS-Based Extraction Followed by High-Performance Liquid Chromatography

Simultaneous Analysis of Ursolic Acid and Oleanolic Acid in Guava Leaves Using QuEChERS-Based Extraction Followed by High-Performance Liquid Chromatography

Effect of the Solvent and the Sample Preparation on the Determination of Triterpene Compounds in Two-Phase Olive-Mill-Waste Samples

Recent advances in the determination of unbound concentration and plasma protein binding of drugs: Analytical methods

Contact Info

Product

Resources

About