Solvent effect on the UV/Vis absorption and fluorescence spectroscopic properties of berberine

Abstract

Spectral and photophysical properties of the alkaloid berberine (B) were studied in solvents with different solvent parameters, using UV/Vis absorption, emission and excitation spectroscopy. The absorption and emission maxima were found to be between 421-431 nm and 514-555 nm, respectively, leading to Stokes' shifts between 4099 and 5735 cm(-1). The fluorescence quantum yields varied between 10(-2)-10(-4), depending on the solvent. Different solvent scales have been used to study the solvatochromism of B. Linear solvation energy relationships (LSER) proposed by Kamlet-Taft suggest that B is a molecule attractive as a probe for solvent polarity and hydrogen bonding properties.

Fabrication of Novel Hydrogel with Berberine-Enriched Carboxymethylcellulose and Hyaluronic Acid as an Anti-Inflammatory Barrier Membrane

“…Berberine content was determined using a NanoDrop UV-Vis Spectrophotometer (ND-1000, Thermo Scientific, Waltham, MA, USA) at 260 nm wavelength as in a previous study [27]. The calibration curve of the berberine standard (B3251, Sigma-Aldrich) was determined from six concentration points over the range of concentrations (37.25 to 1000  μ g/mL) with an R value greater than 0.99.…”

The fluorescence properties and NMR analysis of protopine and allocryptopine

“…for tetrahydroprotoberberines the fluorescence maxima between 320 and 330 nm have been reported [18]. From this point of view, it is important to note that the fluorescence properties of alkaloids studied are solvent dependent, as recently reported for berberine in detail [19]. Spectrometric data on the IQAs also confirm that their absorption and fluorescence parameters are affected both by the aromaticity of the heterocyclic nucleus and the nature and/or position of methylenedioxy, methoxy, and hydroxy substituents.…”

Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

“…The fluorescence of berberine can serve as fluorescent marker. In addition, both absorption and emission of berberine are sensitive to environment and can be used for sensing [34,[37][38][39][40][41][42]. Fluorescence spectra of berberine, ND, ND-berberine complex are shown in Fig.…”

“…1. The absorption spectrum reveals three strong bands with maxima around 250 and 350 nm, which are characterized as  → * transition [34], and forth relatively weak band centered at about 430 nm. In Fig.…”

“…The intensity increasing and the shift of the fluorescence maximum of berberine when berberine interacts with environment have been described and analyzed previously, using non-polar solvents [34], DNA [37,42] and many other bio-organic molecules and systems [e.g. 41].…”