Particle gluing operating parameters in particleboard (PB) production have an important influence on the mechanical properties of PBs. This study developed a multi-objective optimization model based on support vector regression (SVR) optimized by the non-dominated sorted genetic algorithm-II (NSGA2) to realize the multi-objective accurate prediction of PB mechanical properties (modulus of elasticity (MOE), modulus of rupture (MOR), and internal bonding (IB) strength) by adjusting particle gluing operating parameters. The NSGA2-SVR multi-objective prediction model was trained by 496 groups of experimental data of particle gluing operating parameters and PB mechanical properties. The prediction results of the NSGA2-SVR multi-objective prediction model were evaluated by 124 groups of experimental data and compared with the prediction results of the back propagation neural network (BPNN) model, general regression neural network (GRNN) model, and SVR model. The mean absolute percentage errors (MAPEs) of the NSGA2-SVR model were 49.11%, 33.64%, and 24.20% lower than that of the BPNN model, GRNN model, and SVR model, respectively. The Theil’s inequality coefficients (TICs) of the NSGA2-SVR model were 40.93%, 27.39%, and 18.58% lower than that of the BPNN model, GRNN model, and SVR model, respectively. The results showed that the multi-objective prediction model based on NSGA2-SVR has a superior fitting and higher prediction accuracy for the prediction performance of particle gluing operating parameters, and the NSGA2-SVR model can be applied to the multi-objective synchronous prediction of particle gluing operating parameters in the PB production line.