Somaclonal Variation in Date Palm

Hadrami, Abdelbasset El; Daayf, Fouad; Elshibli, Sakina; Jain, S. Mohan; Hadrami, I. El

doi:10.1007/978-94-007-1318-5_9

Cited by 26 publications

(20 citation statements)

References 63 publications

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“…Sometimes micropropagated plants carry advantageous characters with respect to commercial and agronomical interest which finally leads to some new varieties (El Hadrami et al, 2011). Somaclonal variation act as a raw material for the mass propagation, cryopreservation and synthetic seed production.…”

Section: Somaclonal Variationmentioning

confidence: 99%

“…The high concentration of 2,4-D induced 25% somaclonal variation and observed change in the leaf morphology and also cause poor flower pollination leads to low quality date fruit (Fki et al, 2011b). To reduce the risk of somaclonal variation researchers suggest using juvenile explants, low auxin concentration, especially 2,4-D and minimum numbers of subcultures (El Hadrami et al, 2011;Fki et al, 2011aFki et al, , 2011bBi, 2012). The use of high concentration of plant growth regulators induces somaclonal variation in date palm (Al-Mazroui et al, 2007).…”

Section: Somaclonal Variationmentioning

confidence: 99%

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Date palm micropropagation: Advances and applications

Al-Khayri

Naik

2017

Ciênc. agrotec.

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Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature inflorescences of adult trees were utilized as an alternative nondestructive source of explants. In addition to the nature of the explant used, successful plant regeneration depends on the cultivar, composition of the culture medium and physical status. Challenges of date palm micropropagation include long in vitro cycle, latent contamination, browning, somaclonal variation as well as ex vitro acclimatization and transplanting. A remarkable amount of research investigating these factors has led to optimized protocols for the micropropagation of numerous commercially important cultivars. This has encouraged the development of several international commercial tissue culture laboratories. Molecular characterization provides an assurance of genetic conformity of regenerated plantlets, a key feature for commercial production. This article describes date palm micropropagation protocols and also discusses recent achievements with respect to somaclonal variation, molecular markers, cryopreservation and future prospects.Index terms: Cryopreservation; somatic embryogenesis; somaclonal variation; organogenesis; molecular marker. RESUMOA tamareira (Phoenix dactylifera L.) é uma arvore frutífera adaptada à condições climáticas adversas predominantemente em regiões áridas do Oriente Médio e Norte Africano. As tâmaras possuem vários componentes químicos com alto valor medicinal e nutricional. A propagação tradicional por estacas não é suficiente para satisfazer a demanda por mudas e assim, a micropropagação apresenta-se como uma alternativa eficiente para a produção de mudas em larga escala. Embriogênese somática e organogênese, tanto direta quanto indireta via calos, tem sido usada para obter a regeneração in vitro de tamareira. O inicio do cultivo in vitro normalmente utiliza meristemas excisados de brotações jovens. Recentemente, inflorescências imaturas de árvores adultas são usadas como fonte alternativa de explantes não destrutiva. Além da origem do explante, o sucesso da regenerção depende do cultivar, da composição do meio de cultura e de condições físicas. Desafios na micropropagação de tamareira incluem um longo ciclo in vitro, contaminação, escurecimento do tecido, variação somaclonal além do enraizamento e aclimatização ex vitro. Diversos estudos investigando esses fatore...

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Section: Somaclonal Variationmentioning

confidence: 99%

Section: Somaclonal Variationmentioning

confidence: 99%

Date palm micropropagation: Advances and applications

Al-Khayri

Naik

2017

Ciênc. agrotec.

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“…Ferhi using RAPD primers [70]. Low concentrations of auxins, mainly 2,4-D, low numbers of subcultures and the use of juvenile explants were suggested to decrease the risk of somaclonal variation [9,71,72].…”

Section: Genetic Variability In Regenerantsmentioning

confidence: 99%

Micropropagation of Date Palm: A Review

Mazri¹,

Meziani²

2015

Cell Dev Biol

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Somatic embryogenesis and organogenesis are the two pathways of choice for rapid and large-scale propagation of date palm. They have been successfully used for the micropropagation of elite genotypes and have proved their effectiveness for the commercial production of many cultivars. Nevertheless, regeneration through somatic embryogenesis or organogenesis in date palm is still difficult to achieve for recalcitrant genotypes and is often hampered by certain physiological disorders. In the present review, we report the results of a number of studies carried out on date palm micropropagation. It also describes different factors that influence each stage of somatic embryogenesis and organogenesis and the main problems encountered during these two micropropagation processes.

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“…To augment traditional breeding techniques, biotechnology is an innovative approach for date palm improvement (Jain, 2012). So far, various biotechnological techniques have already been used including induced embryo rescue (Sudhersan and Al-Shayji, 2011) in vitro mutagenesis and selection against bayoud disease (Jain, 2006(Jain, , 2007El Hadrami et al, 2005, 2011a, and protoplast culture (Chabane et al, 2007;Rizkalla et al, 2007;Assani et al, 2011). Protoplasts are used for the production of somatic hybrids, and genetic transformation (Saker et al, 2007(Saker et al, , 2009Mousavi et al, 2009;Saker, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…In vitro selection of somaclones against certain stress factors offers a means for the improvement of date palm tolerance to abiotic stresses including drought and salinity as well as biotic agents such as pathogens and pests (El Hdrami et al, 2011a;Jain 2012). El Hadrami et al (2011a) reviewed the reasons for such somaclonal variation and their uses in detail.…”

Section: Introductionmentioning

confidence: 99%

In vitro selection of abiotic stress tolerant date palm (Phoenix dactylifera L.): A review

Al-Khayri

Ibraheem²

2014

Emir. J. Food Agric

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The advent of agricultural biotechnology offers new approaches for the genetic improvement of date palm (Phoenix dactylifera L.), an important economic fruit crop in arid regions. In vitro studies were conducted to recover date palm plants exhibiting enhanced tolerance to salinity and drought stress, two major agricultural problems in arid areas. The response of date palm callus cultures to salt stress was investigated by exposing cell suspension and callus cultures to varying concentrations of sodium chloride (NaCl). The reaction of callus to the duration of exposure to NaCl, potassium chloride (KCl) and calcium chloride (CaCl2) was evaluated. Similarly, drought tolerance was assessed in response to varying concentrations of polyethylene glycol (PEG-8000). Significant differences were reported in various parameters measured including callus growth, somatic embryogenesis, proline content and ion distribution. Calli with enhanced tolerance to these selection agents were obtained; however, plant regeneration from these cell lines was not realized. Although the available data provide an insight of the behavior of date palm callus in the presence of salt and drought stress, further research work is required to refine the selection procedures and plant regeneration from the selected tolerant cell lines.

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Somaclonal Variation in Date Palm

Cited by 26 publications

References 63 publications

Date palm micropropagation: Advances and applications

Date palm micropropagation: Advances and applications

Micropropagation of Date Palm: A Review

In vitro selection of abiotic stress tolerant date palm (Phoenix dactylifera L.): A review

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