Somatic Cell Count: A Biomarker for Early Diagnosis and Therapeutic Evaluation in Bovine Mastitis

Das, Diptasikha; Panda, Saroj K.; Jena, Biswadeep; Sahoo, Arun Kumar

doi:10.20546/ijcmas.2018.703.174

Cited by 8 publications

(8 citation statements)

References 9 publications

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“…Furthermore, the milk SCC in group C was signi cantly higher than that of healthy group (group A), and it is consistent with the research of Das D et al [14]. This indicated that the immune cells were accumulated abundantly in the damaged tissues by the non-speci c and uid immune response.…”

Section: Discussionsupporting

confidence: 90%

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Liu

Qian

et al. 2020

Preprint

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Background To investigate the effect of Pulsatilla saponin B4, a purified extract form Pulsatilla Chinensis, on the treatment of clinical mastitis in dairy cows, 12 healthy cows were used as the control group (group A, no treatment), and 36 cows with mastitis were divided into 3 groups based on the quaque die (QD) intramuscular injection (IM) dose used in the as follows, group B (15 mL), group C (30 mL), group D (60 mL). Identified the microorganisms in milk during the experiment,samples in test groups were cultured, isolated, and verified by quantitative real-time polymerase chain reaction (qPCR). Somatic cell count (SCC) and serum inflammatory indices in cows were measured with a 1-day interval in group C and compared with group A. Results Results showed that bacterial detection rates were 100% (group B), 83.33% (group C) and 100% (group D). After the treatment bacterial detection rates dropped to 50.00% (group B), 50.00% (group C), and 41.67% (group D). The medicine was effective in all test groups and showed a dose-effect relationship. Serum haptoglobin (HP), prostaglandin E2 (PGE2), interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-8 (IL-8), interleukin-10 (IL-10) and tumour necrosis factor alpha (TNF-α), interleukin-4 (IL-4), leukotriene B4 (LTB4) also the SCC were significantly higher than healthy cows (P < 0.05) on the day (d) 1 in group C but had no significant difference with group A after treatment. Conclusion Treatment with Pulsatilla saponin B4 relieved mastitis, leading to relieve of bacterial infection, SCC and levels of serum inflammatory factors effectively in cows with mastitis after being administered for 4–6 days QD.

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Section: Discussionsupporting

confidence: 90%

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Liu

Qian

et al. 2020

Preprint

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“…This formed a composite from which 2 ml of the composite milk sample was then added to 2 ml of CMT reagent on the test paddle and mixed gently to observe reaction. The result was graded as described by various authors [21,22]. All samples that tested positive for subclinical Mastitis were properly labelled and immediately transported to the Bacteriological Analysis Laboratory of the Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria in an ice box for processing.…”

Section: Sample Collection and Screening For Subclinical Mastitismentioning

confidence: 99%

Antimicrobial Susceptibility Profile of Coliforms from Bovine Mastitis Cases among Pastoral Herds in Parts of Kaduna State, Nigeria: Curbing the Environmental Health Risk

Makolo

Suleiman

Olonitola

et al. 2019

AJARR

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Consumption of raw milk from pastoral bovines have been identified as a major source of public and environmental health risk in developing countries. Antimicrobial resistance is a global health challenge threatening the lives of humans and animals. The indiscriminate use of antimicrobial agents among the pastoralists on commercial animals, especially for non-therapeutic purposes has been linked to the development of resistant strains of potentially pathogenic bacteria which are being transferred from animals to humans. This study investigated the antimicrobial susceptibility profile of coliform bacteria isolated from mastitis milk of pastoral herds. Out of 147 milk samples collected and screened for subclinical mastitis, 29 (19.7%) were positive. Out of the 29 mastitis positive samples, 13 (8.8%) were positive for coliforms (6 E. coli and 7 K. pneumoniae). All the coliform isolates showed 100% resistance to Penicillin and Tetracycline, and were all 100% susceptible to Imipenem. High multidrug resistance was expressed by all the isolates to Penicillin, Tetracycline and Erythromycin. All the isolates (100%) had Multiple Antibiotic Resistance Index (MARI) of 0.2 and above which is an indication of gross abuse of antibiotics within the studied population. However, antibiotics still effective against the coliform species tested were Imipenem (100%), Ciprofloxacin (92.3%), Gentamycin (92.3%), Chloramphenicol (84.6%), Amoxicillin/ Clavulanic acid (84.6%) and Sulfamethoxazole/Trimethoprim (69.2%).

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“…SCC re ectd the severity of mastitis, and was used in early diagnosis and treatment evaluation of mastitis in dairy cows [16]. Das et al [17] found that the SCC value was signi cantly higher (P < 0.05) in clinical mastitis cows compared to subclinical mastitis and healthy cows. Our ndings are consistent with them, indicating that the immune cells were accumulated abundantly in the damaged tissues through the non-speci c and uid immune response.…”

Section: Discussionmentioning

confidence: 99%

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Shen

Qian

You

et al. 2020

Preprint

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Background: Bovine mastitis is an inflammatory reaction of the breast caused by pathogenic bacteria infection or breast injury, which affect milk yield and quality and poses severe threat to the health of dairy cows. Pulsatilla chinensis has antibacterial, anti-inflammatory, and immune enhancing effects, etc. However, there was no research about the therapeutic effect of it towards the treatment of cow mastitis. In the study, twelve healthy cows were selected as the control group (group A), and 36 cows with mastitis were equally and randomly divided into group B, C and D. Group A didn’t accept treatment, while group B, C and D were treated with 15, 30 and 60 mL Pulsatilla saponin B4 (a purified extract form Pulsatilla chinensis) injection by brachiocephalicus intramuscular injection once a day for 4-6 day and the optimal dose was selected from them. Then, we investigated the effect of this extract on the clearance rates of pathogenic bacteria, the regulation of somatic cell count (SCC) and inflammatory factors the in appropriate dose group.Results: Pulsatilla saponin B4 was effective in all test groups and showed a dose-effect relationship. The extract had inhibitory effects on Streptococcus agalactiae, Streptococcus dysenteriae, Klebsiella species, Mycoplasma bovis, Escherichia coli and Staphylococcus aureus. Group C cured more cows than group B and D during the 6 days of treatment and was determined as the optimal dose group. The milk SCC, serum haptoglobin (HP), prostaglandin E2 (PGE2), interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-8 (IL-8), interleukin-10 (IL-10) and tumour necrosis factor alpha (TNF-α), interleukin-4 (IL-4) and leukotriene B4 (LTB4) in Group C were significantly higher than those of healthy cows (P < 0.05) on the first day but had no significant difference with group A after treatment. Conclusion: 30 mL Pulsatilla saponin B4 applied through intramuscular injection once a day for 4-6 days leaded to the significant amelioration of bacterial infection, SCC and levels of serum inflammatory factors in cows with clinical mastitis.

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Somatic Cell Count: A Biomarker for Early Diagnosis and Therapeutic Evaluation in Bovine Mastitis

Cited by 8 publications

References 9 publications

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Antimicrobial Susceptibility Profile of Coliforms from Bovine Mastitis Cases among Pastoral Herds in Parts of Kaduna State, Nigeria: Curbing the Environmental Health Risk

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

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