Somatic cells and the G2 to M-phase transition in sheep oocytes

Galli, Cesare; Moor, R. M.

doi:10.1051/rnd:19910202

Cited by 8 publications

(5 citation statements)

References 14 publications

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“…In subsequent studies in our laboratory, however, we have demonstrated that DRB can be replaced at intervals up to 8 h without a significant reduction in its effectiveness (Martus and Farin, 1993). The observation that cumulus cell-oocyte complexes, rather than cumulus-free oocytes, must be present for DRB-mediated inhibition of maturation is consistent with previous studies in the ewe, cow, and pig in which a-amanitin was used to block GVBD (Osborn and Moor, 1983; Moor and Crosby, 1986;Galli and Moor, 1991;Meinecke and Meinecke-Tillmann, 1993).…”

Section: Discussionsupporting

confidence: 89%

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Inhibition of germinal vesicle breakdown in bovine oocytes by 5,6‐dichloro‐1‐β‐D‐ribofuranosylbenzimidazole (DRB)

Farin

Yang

1994

Molecular Reproduction Devel

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5,6-Dichloro-1-beta-D-ribofuranosyl-benzimidazole (DRB) is an analog of the nucleoside adenosine that has been used to inhibit transcription in a variety of cell types. The present studies were designed to evaluate the ability of DRB to block germinal vesicle breakdown (GVBD) in bovine oocytes matured in vitro and to characterize culture conditions required for DRB-mediated arrest of meiosis. Administration of DRB (60-90 microM) at 2-3 h intervals during culture of COC blocked GVBD in approximately 70 percent of oocytes. The inhibitory effect of DRB was reversible and required the presence of cumulus cells. Treatment with DRB was associated with a 57% decrease in 3H-uridine incorporation into total COC RNA and a 90.8% decrease into mRNA but did not affect the incorporation of 3H-leucine into COC proteins. The ability of DRB to arrest meiosis was significantly compromised if supplemental gonadotropin preparations were absent from the maturation media. Gonadotropin-induced GVBD as well as cumulus cell expansion was blocked by treatment with DRB but not with adenosine. GVBD in cultured bovine COC was initially inhibited and then stimulated when supplemental gonadotropin preparations were included in the culture media. DRB treatment in the presence of gonadotropin supplementation blocked the stimulatory effect of gonadotropins on GVBD. In conclusion, DRB can be used to arrest GVBD in bovine COC in a specific and reversible manner. The data support the hypothesis that gene transcription is required for the stimulatory phase of gonadotropin-mediated GVBD in cultured bovine COC.

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Section: Discussionsupporting

confidence: 89%

“…The data presented herein lend support to the hypothesis (Galli and Moor, 1991) that gonadotropins mediate a transcriptional event to initiate GVBD in bovine oocytes. These observations do not imply, however, that in the absence of gonadotropins bovine oocytes will not undergo GVBD.…”

Section: Adenosinesupporting

confidence: 83%

Inhibition of germinal vesicle breakdown in bovine oocytes by 5,6‐dichloro‐1‐β‐D‐ribofuranosylbenzimidazole (DRB)

Farin

Yang

1994

Molecular Reproduction Devel

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“…Second, the metabolic consequences of these signaling events must be transmitted to the oocyte through physical interactions at the follicle cell-oocyte interface. In view of recent evidence suggesting that the oocyte can influence the function of surrounding granulosa cells (Buccione et al, 19901, and that the regulation of meiotic maturation requires transcriptional and translational activity within the cumulus (Galli and Moor, 1991;Motlik et al, 19891, connectivity within this heterocellular complex must now be considered as a complex phenomenon involving bidirectional signaling. It is the purpose of this study to evaluate our current knowledge of the types of physical associations that exist between follicle cells, and the oocyte in mammals based on classical structural analysis.…”

Section: Introductionmentioning

confidence: 99%

Patterns of intercellular connectivity in the mammalian cumulus‐oocyte complex

Albertini

Rider

1994

Microscopy Res & Technique

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Electron and fluorescence microscopic techniques have been used in a complementary fashion to study the patterns of follicle cell-oocyte interactions within cumulus-oocyte-complexes of various mammals. The principal findings are: (1) two distinct types of transzonal processes exist that are distinguishable on the basis of cytoskeletal composition; (2) in some of the species examined (pig, goat, primate), corkscrew-shaped processes rich in tubulin, traverse the zona pellucida and are invaginated into the oocyte cortex; (3) actin-rich processes either ramify as a network at the outer surface of the zona pellucida or penetrate the zona and make contact with the oolemma in a species specific manner. These results are discussed with respect both to the need to employ complementary optical methods in assessing connectivity patterns within COC and to the possible role that extracellular matrix-cell interactions play in the homeostatic control of oocyte growth and maturation.

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“…The rates of in vitro maturation of mammalian oocytes including mice, cattle, and sheep are low compared with oocytes matured in vivo 28‐30 . This might be due to poor nuclear maturation caused by inadequate culture conditions 31‐34 .…”

Section: Discussionmentioning

confidence: 99%

“…The rates of in vitro maturation of mammalian oocytes including mice, cattle, and sheep are low compared with oocytes matured in vivo. [28][29][30] This might be due to poor nuclear maturation caused by inadequate culture conditions. [31][32][33][34] Several factors are included in culture media to improve the success rate of in vitro maturation.…”

Section: Discussionmentioning

confidence: 99%

Effects of estradiol on in vitro maturation of buffalo and goat oocytes

Maksura

Akon

Islam

et al. 2020

Reprod Medicine & Biology

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Assisted reproductive technologies (ARTs) can be applied to improve reproductive efficiencies in livestock species. In addition, ARTs have significant roles in the preservation of endangered species. 1 ARTs including artificial insemination (AI), cryopreservation, intra-cytoplasmic sperm injection (ICSI), multiple ovulation and embryo transfer (MOET), and in vitro embryo production (IVEP) have been used for improvement of domestic animals. In buffaloes, the response to superovulation treatment is poor, and ultimately, MOET is hardly applicable. However, oocytes recovered from slaughtered animals are used for IVEP. In vitro maturation (IVM) is the initial step of IVEP technology. IVM of oocytes involves collection of cumulus oocyte complexes (COCs) from antral follicles and culturing them until they reach to the metaphase II (MII) stage. 2

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Somatic cells and the G2 to M-phase transition in sheep oocytes

Cited by 8 publications

References 14 publications

Inhibition of germinal vesicle breakdown in bovine oocytes by 5,6‐dichloro‐1‐β‐D‐ribofuranosylbenzimidazole (DRB)

Inhibition of germinal vesicle breakdown in bovine oocytes by 5,6‐dichloro‐1‐β‐D‐ribofuranosylbenzimidazole (DRB)

Patterns of intercellular connectivity in the mammalian cumulus‐oocyte complex

Effects of estradiol on in vitro maturation of buffalo and goat oocytes

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