Somatic Embroygenesis in Jack Pine (Pinus Banksiana Lamb)

Park, Y. S.; Bonga, J. M.; Cameron, Stewart I.; Barrett, J.; Forbes, Kathleen; DeVerno, L. L.; Klimaszewska, Krystyna

doi:10.1007/978-94-017-3032-7_21

Cited by 10 publications

(5 citation statements)

References 20 publications

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“…This rate was low but consistent with reported results for Pinus banksiana (0.4%) (Park et al, 1999), Pinus thunbergii (2%) and Pinus densiflora (1%) . In contrast, higher initiation rates were reported for Pinus taeda (17.9%) (Pullman et al, 2003b), Pinus strobus (20 to 53%) (Klimaszewska et al, 2001), Pinus sylvestris (up to 22%), and Pinus pinaster (up to 40%) (Lelu et al, 1999).…”

Section: Discussionsupporting

confidence: 93%

Somatic embryogenesis and plant regeneration in yakutanegoyou, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endemic and endangered species in Japan

Maruyama

Hosoi

Ishii

2007

In Vitro Cell.Dev.Biol.-Plant

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Section: Discussionsupporting

confidence: 93%

Somatic embryogenesis and plant regeneration in yakutanegoyou, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endemic and endangered species in Japan

Maruyama

Hosoi

Ishii

2007

In Vitro Cell.Dev.Biol.-Plant

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“…Shortly after, similar results were obtained for several other species, some examples being P. caribaea (Laine and David 1990), P. radiata (Smith 1996), P. banksiana (Park et al 1998), P. strobus (Garin et al 1998), P. sylvestris and P. pinaster (Lelu et al 1999), and P. nigra (Salajova et al 1999).…”

Section: Introductionsupporting

confidence: 77%

“…1D), and embryogenic tissue was subsequently initiated from the extruded embryo ( Fig 1E). Previously, these tissues have demonstrated that they are capable of producing plants (Park et al 1998;Klimaszewska et al 2001;Lelu et al 1999;Harvengt 2005) and have been successfully cryopreserved.…”

Section: Initiation Of Embryogenic Tissuementioning

confidence: 99%

Initiation of somatic embryogenesis in Pinus banksiana, P. strobus, P. pinaster, and P. sylvestris at three laboratories in Canada and France

Park

Lelu‐Walter²,

Harvengt³

et al. 2006

Plant Cell Tiss Organ Cult

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During 2002During -2004, three laboratories in Canada and France collaborated to improve initiation of somatic embryogenesis (SE) in jack pine (Pinus banksiana Lamb.), eastern white pine (P. strobus L.), maritime pine (P. pinaster Ait.), and Scots pine (P. sylvestris L.), giving particular attention to the effects of (1) N-(2-chloro-4-pyridyl)-N¢-phenylurea (CPPU) versus various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA), (2) differences in basal nutrient media, i.e., macro-and microelements, and (3) gelling agent concentration. The work was carried out separately at each laboratory, but the details of media compositions were shared and tested on their respective species. Results indicate that the developmental stage of the zygotic embryo (ZE) and genotype effects had a large influence on SE initiation, and that genetic effects were consistent over time. Different species responded differently to PGR types and concentration, basal nutrient media, trace elements, and their combinations. Currently, our best initiation rates based on a selected group of genotypes, optimal development stage of ZE, and medium are 3.9% for jack pine, 54.6% for eastern white pine, 76.2% for maritime pine, and 19.7% for Scots pine.

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“…In most pines, SE initiation is limited to the first few weeks of embryo development prior to the emergence of cotyledonary primordia (Finer et al, 1989;Becwar et al, 1990;Lelu et al, 1999;Park et al, 1999;Salajova et al, 1999;Percy et al, 2000). Hence targeting the most responsive stage of embryo development is an important parameter for SE initiation in any pine species.…”

Section: Introductionmentioning

confidence: 99%

Optimized somatic embryogenesis in Pinus strobus L.

Klimaszewska

Park

Overton

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

113

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Somatic embryogenesis (SE) initiation in Pinus strobus was optimized by the manipulation of plant growth regulator (PGR) concentrations in the culture medium. Modified Litvay medium (MLV) of Litvay et al. (1985) supplemented with lower than routinely used PGR concentration increased initiation of established embryogenic cultures from approximately 20 to 53%. The original developmental stage of zygotic embryos had a pronounced effect on the SE response. The optimum stage was the pre-to shortly post-cleavage stage. A substantial genetic influence on initiation of SE was indicated by a significant variance component due to families. Genotype Â collection date and genotype Â media interactions had large effects on initiation of SE. The PGR levels in the culture medium prior to maturation had a significant effect on subsequent production of mature somatic embryos. Embryogenic tissue initiated and proliferated on medium with a low level of PGR consistently produced a high number of somatic embryos, indicating that optimized initiation protocol also enhanced somatic embryo production. Somatic embryos of 93 embryogenic lines (representing five families) that were initiated on media with different PGR concentrations were converted to plants at an overall frequency of 76%, and grown in the greenhouse. With these improved protocols, application of P. strobus SE in commercial clonal forestry is feasible as an alternative to traditional breeding and reforestation.

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Somatic Embroygenesis in Jack Pine (Pinus Banksiana Lamb)

Cited by 10 publications

References 20 publications

Somatic embryogenesis and plant regeneration in yakutanegoyou, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endemic and endangered species in Japan

Somatic embryogenesis and plant regeneration in yakutanegoyou, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endemic and endangered species in Japan

Initiation of somatic embryogenesis in Pinus banksiana, P. strobus, P. pinaster, and P. sylvestris at three laboratories in Canada and France

Optimized somatic embryogenesis in Pinus strobus L.

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