Somatic Embryo from Basal Leaf Segments of Vanda tricolor Lindl. var. pallida

Hardjo, Popy Hartatie; Savitri, Wina Dian

doi:10.18502/kls.v3i5.990

Cited by 8 publications

(10 citation statements)

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“…In orchids, PLBs can be induced directly from explants or indirectly from callus [ 49 , 50 ]. So far, it has not been reported that PLBs of Paphiopedilum can occur directly from explants.…”

Section: Discussionmentioning

confidence: 99%

Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum

et al. 2021

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Background Paphiopedilum, commonly known as slipper orchid, is an important genus of orchid family with prominent horticultural value. Compared with conventional methods such as tillers and in vitro shoots multiplication, induction and regeneration of protocorm-like bodies (PLBs) is an effective micropropagation method in Paphiopedilum. The PLB initiation efficiency varies among species, hybrids and varieties, which leads to only a few Paphiopedilum species can be large-scale propagated through PLBs. So far, little is known about the mechanisms behind the initiation and maintenance of PLB in Paphiopedilum. Results A protocol to induce PLB development from seed-derived protocorms of Paphiopedilum SCBG Huihuang90 (P. SCBG Prince × P. SCBG Miracle) was established. The morphological characterization of four key PLB developmental stages showed that significant polarity and cell size gradients were observed within each PLB. The endogenous hormone level was evaluated. The increase in the levels of indoleacetic acid (IAA) and jasmonic acid (JA) accompanying the PLBs differentiation, suggesting auxin and JA levels were correlated with PLB development. Gibberellic acid (GA) decreased to a very low level, indicated that GA inactivation may be necessary for shoot apical meristem (SAM) development. Comparative transcriptomic profiles of four different developmental stages of P. SCBG Huihuang90 PLBs explore key genes involved in PLB development. The numbers of differentially expressed genes (DEGs) in three pairwise comparisons (A vs B, B vs C, C vs D) were 1455, 349, and 3529, respectively. KEGG enrichment analysis revealed that DEGs were implicated in secondary metabolite metabolism and photosynthesis. DEGs related to hormone metabolism and signaling, somatic embryogenesis, shoot development and photosynthesis were discussed in detail. Conclusion This study is the first report on PLB development in Paphiopedilum using transcriptome sequencing, which provides useful information to understand the mechanisms of PLB development.

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“…In orchids, PLBs can be induced directly from explants or indirectly from callus [ 49 , 50 ]. So far, it has not been reported that PLBs of Paphiopedilum can occur directly from explants.…”

Section: Discussionmentioning

confidence: 99%

Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum

et al. 2021

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“…Wimber [73] was the first to use leaf segments successfully for in vitro orchid propagation. The leaf culture in monopodial orchids is beneficial as no mother plant is sacrificed, and the explants are available throughout the season [74]. Chookoh et al [75] showed only the inner expanding leaves of Tolumnia Snow Fairy producing protocorm-like bodies (PLBs) on MS medium augmented with 4 mg L −1 BA and 0.5 mg L −1 NAA, while outer leaves showed no in vitro growth response.…”

Section: Micropropagation Employing Different Explantsmentioning

confidence: 99%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

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Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation.

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“…Basal segment of leaf was cultured on half-strength MS medium + 0.05 mg L -1 NAA + 0.01 mg L -1 BAP (following Hardjo and Savitri, 2017). Subculturing was carried out after 4 wk (week)s intervals.…”

Section: 2 Induction and Regeneration Of Somatic Embryomentioning

confidence: 99%

“…Auxins, especially α-naphtalene acetic acid (NAA) affect the process of regeneration in monopodial orchids, and act synergistically on the formation of PLBs (Jainol and Gansau, 2017). Hardjo and Savitri (2017) succeeded in callus induction from the basal part of V. tricolor var. pallida's leaf using half-strength MS medium (Melviana, et al, 2021b;Murashige and Skoog, 1962) with the addition of 0.05 mg L -1 NAA in collaboration with 0.01 mg L -1 BAP.…”

Section: Introductionmentioning

confidence: 99%

“…pallida's leaf using half-strength MS medium (Melviana, et al, 2021b;Murashige and Skoog, 1962) with the addition of 0.05 mg L -1 NAA in collaboration with 0.01 mg L -1 BAP. Callus induction method which has been done by Hardjo and Savitri (2017) will be used and developed to induce somatic embryos and then to regenerate them into plantlets. The effect of NAA and BAP was studied for induction and maturation of somatic embryos.…”

Section: Introductionmentioning

confidence: 99%

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Callus-mediated Somatic Embryogenesis and Plant Regeneration in Vanda tricolor Lindl. var. Pallida

2021

JJBS

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In this study, a protocol to induce indirect somatic embryogenesis from the basal leaf segments of Vanda tricolor Lindl. var. Pallida has been developed. 2 0 T The experiments consisted of two stages، i.e. induction of SEs from calli and regeneration of plantlet from SEs. 2 0 T Embryogenic calli obtained from previous experiment (0.05 mg LP -1 P NAA + 0.01 mg LP -1 P BAP) were used to induce somatic embryos (SEs) on half-strength Murashige and Skoog (MS) medium supplemented with (0.05 mg LP -1 P toP P 0.20 mg LP -1 P ) 6-benzylaminopurine (BAP) alone or in combination with 0.01 mg LP -1 P α-naphtalene acetic acidP P (NAA). Embryogenic calli, those cultured on 0.05 mg LP -1 P BAP and 0.01 mg LP -1 P NAA resulted in 90 % induction of SE structures at 30 d of culture period. Histological observation exhibited development of pro-embryo to form completed embryo. The pattern of SEs development started from embryogenic callus to form pro-embryo, followed by globular phase at 10 d of culture. Globular embryo elongated to form suspensor at 20 d of incubation period, and completed embryo. Regeneration of SEs into complete plantlets was attained on the half-strength MS medium without addition of any plant growth regulator (PGR). Based on the results of the present study, it can be concluded that 2 0 T half-strength MS medium supplemented with 2 0 T NAA 0.01 mg LP -1

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Somatic Embryo from Basal Leaf Segments of Vanda tricolor Lindl. var. pallida

Abstract: Somatic embryogenesis is one of techniques in plant micropropagation. The induction of somatic embryogenesis through callus phase was done on Vanda tricolor Lindl. var. pallida. This study aimed to find out the effect of naphtalene acetic acid (NAA) and benzyl amino purine (

Cited by 8 publications

References 4 publications

Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum

Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Callus-mediated Somatic Embryogenesis and Plant Regeneration in Vanda tricolor Lindl. var. Pallida

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