Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants

Orbes, Maria Yumbla; Cruz, Ana Cláudia Ferreira da; Pinheiro, Marcos Vinícius Marques; Rocha, Diego Ismael; Batista, Diego Silva; Koehler, Andréa Dias; Barbosa, José Geraldo

doi:10.1007/s11627-017-9800-2

Cited by 17 publications

(7 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Successful induction of SE was also achieved on root explants of G. lutea grown on a medium supplemented with auxins alone or in combination with cytokinin, although the conversion of somatic embryos into plantlets required the addition of mannitol or sorbitol to the basal culture medium [ 55 ]. The initiation of somatic embryos was also obtained in root explants of Eustoma grandiflorum cultured on a medium with 2,4-D [ 56 ]. The somatic embryos originated from pericycle and vascular parenchyma cells of seedling roots.…”

Section: Se From Centaury Root Explants Is Spontaneous and Directmentioning

confidence: 99%

See 1 more Smart Citation

Somatic Embryogenesis in Centaurium erythraea Rafn—Current Status and Perspectives: A Review

Simonović

Trifunović‐Momčilov

Filipović

et al. 2020

Plants

View full text Add to dashboard Cite

Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum of secondary metabolites with confirmed healing properties. Centaury is an emerging model in plant developmental biology due to its vigorous regenerative potential and great developmental plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic embryos. When SE is initiated from centaury root explants, the process occurs spontaneously (on hormone-free medium), directly (without the callusing phase), and the somatic embryos are of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is indirect (preceded by callusing). Histological observations and culture conditions are compared in these two systems. The changes in antioxidative enzymes were followed during SE from the leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were analyzed using a variety of approaches. The newest and preliminary results, including centaury transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed as perspectives of centaury research.

show abstract

Section: Se From Centaury Root Explants Is Spontaneous and Directmentioning

confidence: 99%

“…The somatic embryos originated from pericycle and vascular parenchyma cells of seedling roots. Further conversion of the somatic embryos into plantlets was enabled with the addition of BA or GA 3 [ 56 ].…”

Section: Se From Centaury Root Explants Is Spontaneous and Directmentioning

confidence: 99%

Somatic Embryogenesis in Centaurium erythraea Rafn—Current Status and Perspectives: A Review

Simonović

Trifunović‐Momčilov

Filipović

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…Somatic embryogenesis and shoot organogenesis from root explants of Lisianthus (Eustoma grandiflorum) could be alternatively induced by auxin or cytokinin. Somatic embryonic calli was induced in the dark on the MS medium supplemented with 10 μM 2,4-D and then the somatic embryonic calli were transferred to an embryo conversion phase medium containing 2 μM BA to promote full plantlet development 43 .…”

Section: Histological Observation Of Somatic Embryogenesis From Leaf mentioning

confidence: 99%

Shoot organogenesis and somatic embryogenesis from leaf and root explants of Scaevola sericea

Liang

Xiong

Guo

et al. 2020

Sci Rep

View full text Add to dashboard Cite

An efficient regeneration system via shoot organogenesis and somatic embryogenesis from in vitro leaf and root explants was established for Scaevola sericea for the first time. The highest axillary shoot proliferation coefficient (4.8) was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 6-benzyladenine (BA) and 0.1 mg/L α-naphthaleneacetic acid (NAA) every 45 days. Young in vitro leaves and roots, which were used as explants, were cultured onto medium supplemented with different plant growth regulators. Our results showed that only cytokinins BA and thidiazuron (TDZ), could induce adventitious shoots and somatic embryos from leaf and root explants. The optimal medium to achieve this was MS medium supplemented with 2.5 mg/L BA and which induced most adventitious shoots (2.7) and somatic embryos (17.3) from leaf explants within 30 days. From root explants, 1.1 adventitious shoots and 7.6 somatic embryos could be induced on MS medium supplemented with 2.5 mg/L TDZ. Histological observation showed that both somatic embryos and adventitious shoots were originated from homogeneous parenchyma and the development of somatic embryos was visible. Maximum rooting percentage (99.0%) was achieved on half-strength MS medium supplemented with 2.5 mg/L NAA. Well-rooted plantlets, which were transplanted into a substrate of pure river sand, displayed a high survival percentage of 91.7% after transplanting for 45 days while the best substrate for plantlet growth was river sand: coral sand (1:1). Abbreviation MS Murashige and Skoog medium 2,4-D 2,4-Dichlorophenoxyacetic acid NAA α-Naphthaleneacetic acid TDZ Thidiazuron BA 6-Benzyladenine KIN Cytokine PGRs Plant growth regulators Scaevola sericea Vahl (syn. Scaevola taccada (Gaertn.) Roxb.), commonly known as beach naupaka, is a semimangrove tree from the Goodeniaceae that consists of 13 genera with 329 accepted species 1 , and almost all the original and existing species are concentrated in Australia 2. Only the genus Scaevola is widespread throughout the world 3. There are a total of 300 scientific plant names of species rank for the genus Scaevola, 40 of which are distributed outside Australia, mostly in tropical island areas 4. S. sericea is a dominant plant at the leading edge of beach communities throughout much of the tropical Pacific 5,6. S. sericea has beautiful flowers with a long flowering period, beautiful branch type and leaves, so it is often bred as an ornamental and landscape plant 7,8 .

show abstract

“…In contrast to roots explants of C. erythrea , both somatic embryos and buds, which developed on seedling roots of Eustoma grandiflorum , had the same histological origin and occurred through the reactivation of pericycle and vascular parenchyma cells (Yumbla-Orbes et al, 2017). However, initiation of SE was stimulated by 10 μM 2,4-D and embryo converted into plantlets thanks to the addition of 2–4 μM BA or 1.5 μM GA 3 while organogenesis occurred on media supplemented with 4 or 8 μM BA, 4 μM zeatin, or high TDZ concentrations (8–12 μM).…”

Section: Explants Considered For Embryogenic Culture Initiationmentioning

confidence: 99%

Somatic Embryogenesis in the Family Gentianaceae and Its Biotechnological Application

et al. 2019

View full text Add to dashboard Cite

The family Gentianaceae consists of 1736 species, which play an important role in human being existence due to their pharmacological and horticultural values. Many species accumulate bitter iridoid substances used medicinally and in flavorings, while others are cultivated because of beauty of their flowers showing a wide range of colors and patterns. Out of 99 genera belonging to the gentian family, process of somatic embryogenesis (SE) was reported for 5. The first reports, aimed at micropropagation of ornamental cultivars and production of secondary metabolites, concerned Centaurium erythraea Rafn., Eustoma russellianum Grieseb. and Exacum affine Balf. Somatic embryos were induced on different explants cultured in the liquid Murashige and Skoog medium supplemented with auxins and cytokinins. In the 1990s of the last century, significant progress in the exploration of the phenomenon of SE and its biotechnological application was made for the genus Gentiana . The process was induced on various explants and studied at the structural and ultrastructural levels. Regenerated plants were screened for genetic stability using flow cytometry, chromosome counting, and molecular markers. Besides typical indirect SE, the use of leaf fragments enabled to obtain single-cell origin of somatic embryos. On the other hand, proliferation of embryogenic callus in liquid medium resulted in the establishment of long-term embryogenic cell suspension cultures, paving the way not only to study the formation of somatic embryos and the development of regenerants but also to preserve the morphogenic potential of cell aggregates by cryopreservation. Cell suspensions re-established after storage in liquid nitrogen maintained their embryogenic character and allowed to obtain somatic embryo-derived regenerants that were true-to-type at both genetic and epigenetic levels. Another application of SE was related to genetic manipulation purposes. Efficient protocols of plant regeneration from callus-, cell suspension-, or leaf mesophyll-derived protoplasts allowed engaging procedures of somatic hybridization or protoplast electroporation for gentian genome modifications. Also, high embryogenic potential existing in the numerous gentian species enabled successful Agrobacterium -mediated transformation of G. cruciata L. and G. dahurica Fisch.

show abstract

Somatic embryogenesis and de novo shoot organogenesis can be alternatively induced by reactivating pericycle cells in Lisianthus (Eustoma grandiflorum (Raf.) Shinners) root explants

Cited by 17 publications

References 47 publications

Somatic Embryogenesis in Centaurium erythraea Rafn—Current Status and Perspectives: A Review

Somatic Embryogenesis in Centaurium erythraea Rafn—Current Status and Perspectives: A Review

Shoot organogenesis and somatic embryogenesis from leaf and root explants of Scaevola sericea

Somatic Embryogenesis in the Family Gentianaceae and Its Biotechnological Application

Contact Info

Product

Resources

About