Somatic Embryogenesis and Plantlet Regeneration in an Agronomically Important Wild Rice Species Oryza nivara

Callus induction and regeneration in selected indica genotypes of rice (Oryza sativa L.)

“…It was observed that the callus induction frequency increased with the increasing concentration of 2,4-D and decreasing kinetin concentration. Further increase in 2,4-D concentration from 2.0 mg/l, reduced the callus induction frequency which was also noticed by Jaseela et al (2009). Decrease of 2,4-D concentration from 2.0 mg/l led to rhizogenesis in the callus induction medium itself as also observed by Shanthi et al, (2010).…”

Conversion of Banana Synseed Influenced by the Bead Type and Seed Coat

“…The previous reports indicated that conversion of synseeds depends on several factors where the conversion took place under in vitro or ex vitro conditions [6,13,20]. In this work, conversion of synseeds on a sterilized mixture of peat and sand (2:1 v/v), and incubated under tissue culture conditions indicated that douple layered beads (especially where the explants covered only with one layer of artificial endosperm) were better than one layered beads (data not shown) but still it needs further studies to improve the ex vitro conversion of banana synseeds.…”

“…The uniformity of growth of in vitro obtained plants makes it possible to control the flowering time and harvesting. Also, they express higher survival rate in the field, and give a significant increase in yield and fruit quality [5,6], but sumaclonal variation should be avoided [7,8].…”

Improved, Efficient and Reliable Plant Regeneration Protocol for a Recalcitrant Black Rice (Oryza sativa cv. Chakhao amubi)

“…Significant increase in callus induction frequency achieved with using 3 mg/L of 2,4-D in both the case of CIMI and CIMII. 2,4-D is an excellent source of auxin for induction of embryogenic callus, and this is supported by several previous studies (12,(15)(16)(17)(18)(19). Seed sterilizing agent plays an important role in tissue culture protocol, it not only used for seed surface sterilization but also effects the callus induction and their frequency.…”