Somatic embryogenesis and somaclonal variation in Norway spruce: morphogenetic, cytogenetic and molecular approaches

Simplified in vitro propagation protocol for Tacca leontopetaloides (L.) Kuntze and assessment of genetic uniformity of regenerated plantlets

“…Somaclonal variation is a very complex phenomenon, which requires the use of different approaches to be correctly evaluated (Fourre et al, 1997). Analysis of ploidy level variation is a useful complementary approach to molecular analysis, which only enables revealing mutations linked to these markers; whilst genomic changes might remain undetected (Jin et al, 2008;Raimondi et al, 2001).…”

Potential use of RAPD markers in characteristics of cucumber (Cucumis sativus L.) haploids and double-haploids

“…The applicability of the RAPD methodology for the documentation of somaclonal variation is a subject of discussion (Anastassopoulos and Kleil 1996;Fourre et al 1997;Saker et al 2000;Phong et al 2001;Tang 2001). The results of the tests are highly dependent on the number of the primers used and the number of plant lines.…”

Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

“…In vitro regenerated plants may exhibit somaclonal variation as a result from genetic or epigenetic modifications (Fourré et al, 1997;Isabel et al, 1996). It is generally accepted that morphological, cytological and molecular variations may be generated by the imposed stress during in vitro or cryopreservation processes.…”