Somatic Embryogenesis and the Technology of Synthetic Seed

Gray, D. J.; Compton, Michael E.; Harrell, R. C.; Cantliffe, Daniel J.

doi:10.1007/978-3-662-03091-2_9

Cited by 34 publications

(9 citation statements)

References 92 publications

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“…In the second phase embryos pass through the typical stages of zygotic embryogenesis, i.e., globular, heart-shaped and torpedo-shaped stages in dicots, globular, scutellar (transition), and coleoptilar stages in monocots, or globular, early cotyledonary and late cotyledonary embryos in conifers (Gray et al 1995;Toonen and de Vries 1996;Dong and Dunstan 2000).…”

Section: Changes In Endogenous Hormone Contents During Expression Of Sementioning

confidence: 99%

Involvement of Plant Hormones and Plant Growth Regulators on in vitro Somatic Embryogenesis

2005

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In spite of the importance attained by somatic embryogenesis and of the many studies that have been conducted on this developmental process, there are still many aspects that are not fully understood. Among those features, the involvement of plant hormones and plant growth regulators on determining the conversion of somatic onto embryogenic tissues, and on allowing progression and maturation of somatic embryos, are far away from being completely comprehended. Part of these difficulties relies on the frequent appearance of contradictory results when studying the effect of a particular stimulus over a specific stage in somatic embryogenesis. Recent progress achieved on understanding the interaction between exogenously added plant growth regulators over the concentration of endogenous hormones, together with the involvement of sensitivity of the tissues to particular hormone groups, might help clarifying the occurrence of divergent patterns in somatic embryogenesis, and in tissue culture in general. The aspects described above, emphasizing on the effect of the concentration of plant hormones and of the addition of plant growth regulators during the different phases of somatic embryogenesis, will be reviewed in this paper. Citations will be limited to review articles as much as possible and to individual articles only in those cases in which very specific or recent information is presented.Abbreviations: 2,4-D -2

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Section: Changes In Endogenous Hormone Contents During Expression Of Sementioning

confidence: 99%

Involvement of Plant Hormones and Plant Growth Regulators on in vitro Somatic Embryogenesis

2005

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“…Use of somatic embryos as a seeding system has potential advantages, including low process cost, a large number of synthetic seeds produced in a limited space, better maintenance of genetic stability (Gray et al, 1995), rapid multiplication, and potential for direct planting (Cantliffe et al, 1987;Fujii et al, 1987). Consistent production of a large quantity of high-quality somatic embryos is a major problem for the commercial adoption of synthetic seed technology.…”

Section: Introductionmentioning

confidence: 99%

Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato

Cantliffe

2001

In Vitro Cell.Dev.Biol.-Plant

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Somatic embryos of sweet potato have potential as synthetic seeds. The effects of abscisic acid (ABA) (0.0, 0.1, 1.0, 10.0 and 50.0 mM) were examined to improve synchrony and proliferation of somatic embryos. Transferring embryonic cultures at day 7 to solid medium with 0.1 or 1.0 mM ABA produced more torpedo and cotyledonary somatic embryos compared to those cultures transferred at day 0. The development of embryos in suspension culture supplemented with ABA was poor. However, when calli proliferation cultures were in gelled medium and pulsed with 0.1 mM ABA for 14 d, the number of somatic embryos increased. Proembryonic masses cultured in mannitol-containing medium Y 21X5 MPa increased embryo development and synchrony of embryo development. Thus, in this work ABA and mannitol have been shown to improve both the total number and the synchrony of sweet potato somatic embryos.

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“…The formation of SEs is, thus, a unique mode of in vitro regeneration and offers many advantages, including the potential for unlimited production of clones with elite traits. The culture of plant tissues via SE on a large scale makes it possible to produce synthetically coated seeds (Redenbaugh 1993;Gray et al 1995). Secondly, the use of embryogenic cultures has proven to be especially valuable in providing a source of regenerable protoplasts (Chang and Wong 1994;Jimenez 1996).…”

Section: Introductionmentioning

confidence: 99%

Cultural conditions affect somatic embryogenesis in Catharanthus roseus L. (G.) Don

Aslam

Mujib

Samar

et al. 2008

Plant Biotechnol Rep

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We established an efficient plant regeneration system for Catharanthus roseus L. (G.) Don through somatic embryogenesis. Embryogenic callus was induced from hypocotyl of seed germinated in vitro. Somatic embryogenesis in Catharanthus has been categorized into three distinct stages: (1) initiation and proliferation of embryo; (2) maturation, and; (3) germination or plantlet conversion. Beside plant growth regulators, various stages of embryogenesis were screened for their response to a wide variety of factors (pH, gelrite, light, sugar alcohols, polyethyleneglycol and amino acids), which affect embryogenesis. All of the tested factors had a small to marked influence on embryogeny and eventual conversion to plantlets. The plantlets were acclimatized successfully in a greenhouse. To our knowledge, this is the first report describing a detailed study of various cultural factors which regulate embryogenesis in C. roseus. The results discussed in this paper may be used in mass propagation to produce medicinal raw material, and the embryo precursor cells could be used in genetic modification programmes that aim to improve the alkaloid yield as well.

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Somatic Embryogenesis and the Technology of Synthetic Seed

Cited by 34 publications

References 92 publications

Involvement of Plant Hormones and Plant Growth Regulators on in vitro Somatic Embryogenesis

Involvement of Plant Hormones and Plant Growth Regulators on in vitro Somatic Embryogenesis

Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato

Cultural conditions affect somatic embryogenesis in Catharanthus roseus L. (G.) Don

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