Somatic embryogenesis, cell suspension, and genetic stability of banana cultivars

Morais-Lino, Lucymeire Souza; Santos-Serejo, Janay Almeida dos; Amorim, Édson Perito; Santana, José Raniere Ferreira de; Pasqual, Moacir; Silva, Sebastião de Oliveira e

doi:10.1007/s11627-015-9729-2

Cited by 21 publications

(9 citation statements)

References 36 publications

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“…These results are consistent with those reported by Dwivedi et al (2016a, b), who set CSC of Stevia rabaudiana in MS medium added with BAP and NAA. However, most studies on the establishment of CSC indicate that the use of 2,4-dichloro phenoxyacetic acid (2,4-D) is essential as PGR (Mathur and Shekhawat 2013;Kumar et al 2016;Morais-Lino et al 2016). In addition, our study contrasts with the report by Havkin-Frenkel et al (1996), who used the V and W medium (Vacin and Went 1949) in the establishment of CSC in V. planifolia.…”

Section: Discussioncontrasting

confidence: 78%

“…In this context, CSC are an effective tool for various biotechnological applications, such as the production of secondary metabolites (Xu et al 2011;Dwivedi et al 2016a, b;Giri, Zaheer, 2016), in vitro selection of cells resistant to biotic or abiotic stress (Mahlanza et al 2013;Naliwajski and Skłodowska 2013;Barbasz et al 2016), massive micropropagation (Morais-Lino et al 2016), gene transfer, and investigations on cell pathways (Dubrovina et al 2016), protoplast isolation and fusion (Cai and Kang 2014;Mujib et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

Ramírez-Mosqueda

Iglesias-Andreu

2017

3 Biotech

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The establishment and characterization of cell suspension cultures are an in vitro culture technique very useful for various plant biotechnological applications (production of secondary metabolites, mass micropropagation, protoplast isolation and fusion, gene transfer and the investigation of cell pathways). The objective of this study was to establish and characterization of cell suspension cultures of V. planifolia by inducing friable calluses. For that, friable calluses were obtained from immature seeds cultivated in MS medium supplemented with 0.45 µM thidiazuron (TDZ). The effect of benzyladenine (BA) in different concentrations was evaluated. Cultures were incubated under photoperiod at continuous stirring at 120 rpm on an orbital shaker. The optimal condition found for biomass growth in suspension cultures was 0.5 g of inoculum density (fresh weight) in MS liquid, supplemented with 8.88 µM BA. The growth kinetics of the cell suspension culture revealed a maximum cell growth (exponential growth phase) at day 16 and an 80% cell viability. The establishment and characterization of cell suspension cultures of V. planifolia constitute the bases of future studies and above all a better biotechnological use of this crop.

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Section: Discussioncontrasting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

Ramírez-Mosqueda

Iglesias-Andreu

2017

3 Biotech

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“…The values of genetic instability in this study (≤ 10 %) correspond to absent to low levels of variation reported for SE (0 -10 %) as in Cassia occidentalis, Ipomoea batatas, Picea abies, Distylium chinense, Quercus robur, Psidium guajava, Olea maderensis, O. europea, etc. (Fourré et al 1997, Lopes et al 2006, Valladares et al 2006, Triqui et al 2008, Yang et al 2008, Mehta et al 2011, Rai et al 2012, Bobadilla et al 2013, Li et al 2014, Carra et al 2016, Morais-Lino et al 2016, Naz et al 2016, Vinoth and Ravindhran 2016. It is possible that the explants selected may be less susceptible to genetic instability (DeVerno et al 1999) and, as a consequence, the results differ from those reported by others using the same morphogenetic route with intermediate levels of variation (17 -31 %) (Müller et al 1990, López et al 2004, Wilhelm et al 2005, Burg et al 2007, Prado et al 2010, Dey et al 2015.…”

Section: Resultsmentioning

confidence: 98%

Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbata

Chávez-Cortázar¹,

Mata-Rosas²,

Oyama³

et al. 2020

Biologia plant.

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The utility of plant tissue culture for the mass propagation of trees is well known, but continuous in vitro multiplication of plant material may increase the possibility of somaclonal variation; therefore, it is essential to evaluate the genetic integrity of regenerants from species-specific in vitro protocols prior to mass production and implementation. The objectives of this study were: 1) to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration over two cycles of secondary somatic embryogenesis in Magnolia dealbata; and 2) to verify the genetic stability of the regenerants obtained. The embryogenic response was not significantly affected by the concentration of 2,4-D but did vary across cycles of induction. The addition of 4.52 μM 2,4-D induced the highest total number of embryos (100.5), the mean number of somatic embryos (25.1) and somatic embryos per explant (80.6). In both 2,4-D concentration (2.26 or 4.52 μM), genetic integrity between the donor and the propagated clones was 0.90, and the low genetic instability (≤ 0.10 in both PGR treatments) might be due to effect of cyclic somatic embryogenesis or the different response of the explants at stress in in vitro culture conditions. However, it is necessary to examine more cell lines and somatic embryogenesis cycles.

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“…The white translucent callus containing proembryogenic mass on the surface was considered as embryogenic calli and used for establishment of cell suspension cultures as suggested by earlier workers (Ganapathi et al, 1999;Cöte et al, 1996;Khalil et al, 2002;Morais-Lino et al, 2016;Kumaravel et al, 2017;Vargas et al, 2018;Enriquz-Valencia et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Morphological and Ultrastructure of Developmental Stages of Somatic Embryos of Popular Banana Cultivars

Debbarma¹,

Sudhakar²,

Kumar³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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Somatic embryogenesis, cell suspension, and genetic stability of banana cultivars

Cited by 21 publications

References 36 publications

Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbata

Morphological and Ultrastructure of Developmental Stages of Somatic Embryos of Popular Banana Cultivars

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