Somatic Embryogenesis from 20 Open-Pollinated Families of Portuguese Plus Trees of Maritime Pine

Miguel, Celia María Gonzalo; Gonçalves, Sónia; Tereso, Susana; Marum, Liliana; Marôco, João; Oliveira, M. Margarida

doi:10.1023/b:ticu.0000007253.91771.e3

Cited by 83 publications

(63 citation statements)

References 21 publications

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“…Embryogenic cell lines (ECLs) of P. pinaster were established from immature zygotic embryos, as previously described (Miguel et al 2004). The zygotic embryos were excised from seeds collected from open-pollinated trees growing in Mata do Escaroupim, Portugal.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…All the somatic embryogenesis-derived material used in this study was obtained from ECLs established in years 2000, 2001 and 2002. Established ECLs were maintained on proliferation medium consisting of DCR salts and vitamins (Gupta and Durzan 1985) supplemented with 4.4 lM BAP, 14 lM 2,4-D, casein hydrolysate (500 mg/l), L-glutamine (1,460 mg/l) and 2% (w/v) sucrose, solidified with 0.2% (w/v) Phytagel (Sigma), in the dark at 22 ± 2°C (Miguel et al 2004). …”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…Emblings with a welldeveloped epicotyle were transferred to a mixture of peat:perlite:pine tree bark (1:1:1) and acclimatized in a growth chamber over a 10-day period (Miguel et al 2004). After 4 and 12 months under ex vitro conditions (nursery and outdoors) morphology of the emblings was examined, and phenotypes were characterized.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…Somatic embryogenesis in Portuguese genotypes of maritime pine was previously established from zygotic embryos of half-sib families (Miguel et al 2004). Following the established procedure, the SE process takes a minimum of 9 months to complete.…”

Section: Introductionmentioning

confidence: 99%

“…Despite several published studies on SE of P. pinaster (Lelu et al 1999;Miguel et al 2004;Harvengt 2005;Lelu-Walter et al 2006) and the high level of commercial interest in application of this propagation tool, there is no information currently available regarding genetic conformity of cloned plants from in vitro culture.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

et al. 2009

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Somatic embryogenesis (SE) is a propagation tool of particular interest for accelerating the deployment of new high-performance planting stock in multivarietal forestry. However, genetic conformity in in vitro propagated plants should be assessed as early as possible, especially in long-living trees such as conifers. The main objective of this work was to study such conformity based on genetic stability at simple sequence repeat (SSR) loci during somatic embryogenesis in maritime pine (Pinus pinaster Ait.). Embryogenic cell lines (ECLs) subjected to tissue proliferation during 6, 14 or 22 months, as well as emblings regenerated from several ECLs, were analyzed. Genetic variation at seven SSR loci was detected in ECLs under proliferation conditions for all time points, and in 5 out of 52 emblings recovered from somatic embryos. Three of these five emblings showed an abnormal phenotype consisting mainly of plagiotropism and loss of apical dominance. Despite the variation found in somatic embryogenesis-derived plant material, no correlation was established between genetic stability at the analyzed loci and abnormal embling phenotype, present in 64% of the emblings. The use of microsatellites in this work was efficient for monitoring mutation events during the somatic embryogenesis in P. pinaster. These molecular markers should be useful in the implementation of new breeding and deployment strategies for improved trees using SE.

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Section: Somatic Embryogenesismentioning

confidence: 99%

Section: Somatic Embryogenesismentioning

confidence: 99%

Section: Somatic Embryogenesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

et al. 2009

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Somatic Embryogenesis in Pinus spp.

Montalbán

García-Mendiguren

Moncaleán

2016

In Vitro Embryogenesis in Higher Plants

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Somatic embryogenesis (SE) has been the most important development for plant tissue culture, not only for mass propagation but also for enabling the implementation of biotechnological tools that can be used to increase the productivity and wood quality of plantation forestry. Development of SE in forest trees started in 1985 and nowadays many studies are focused on the optimization of conifer SE system. However, these advances for many Pinus spp. are not sufficiently refined to be implemented commercially. In this chapter, a summary of the main systems used to achieve SE in Pinus spp. is reported.

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Advances in Conifer Somatic Embryogenesis Since Year 2000

Klimaszewska

Hargreaves

Lelu‐Walter³

et al. 2016

Methods in Molecular Biology

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This review compiles research results published over the last 14 years on conifer somatic embryogenesis (SE). Emphasis is placed on the newest findings that affect the response of seed embryos (typical explants) and shoot primordia (rare explants) to the induction of SE and long-term culture of early somatic embryos. Much research in recent years has focused on maturation of somatic embryos, with respect to both yield and quality, as an important stage for the production of a large number of vigorous somatic seedlings. Attempts to scale up somatic embryo production numbers and handling have resulted in a few bioreactor designs, the utility of which may prove beneficial for an industrial application. A few simplified cryopreservation methods for embryonal masses (EM) were developed as a means to ensure cost-efficient long-term storage of genotypes during clonal field testing. Finally, recent long-term studies on the growth of somatic trees in the field, including seed production yield and comparison of seed parameters produced by somatic versus seed-derived trees, are described.

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Somatic Embryogenesis from 20 Open-Pollinated Families of Portuguese Plus Trees of Maritime Pine

Cited by 83 publications

References 21 publications

Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

Somatic Embryogenesis in Pinus spp.

Advances in Conifer Somatic Embryogenesis Since Year 2000

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