Somatic embryogenesis from immature inflorescences of oil palm

Teixeira, J. B.; Söndahl, M. R.; Kirby, Edward G.

doi:10.1007/bf00233313

Cited by 92 publications

(69 citation statements)

References 8 publications

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“…Rapid medium and explant browning is common during the establishment of Piper cultures, and is mainly due to the presence of phenolics that under oxidation produce growth inhibitor polyphenols and quinones (Madhusudhanan & Rahiman 2000). Activated charcoal can adsorb inhibitory compounds secreted from plant tissues, and thus reduce their effect (Teixeira et al 1994). In order to control polyphenol leaching, activated charcoal was supplemented into the culture medium.…”

Section: Resultsmentioning

confidence: 99%

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

et al. 2009

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RESUMO -(Morfogênese in vitro e estabelecimento de culturas de suspensão celular em Piper solmsianum C. DC. (Piperaceae)). Piper solmsianum é uma espécie herbácea do sudeste brasileiro onde vários compostos biologicamente ativos já foram identificados. O objetivo deste trabalho foi estabelecer suspensões celulares nesta espécie. Para tanto, foram utilizados explantes de pecíolos e folhas, retirados de plântulas cultivadas in vitro, os quais foram submetidos a diferentes combinações de reguladores de crescimento (AIA, ANA, 2,4-D e BAP). Foi obtida a neo-formação de raízes e brotos, estes últimos através do processo de organogênese indireta evidenciada por estudos histológicos. Para a indução e crescimento dos calos, foram avaliados, além das diferentes combinações de reguladores de crescimento, a suplementação ao meio de cultura de carvão ativado (1,5 mg.l -1 ) e o regime de luz. Culturas mantidas na luz, em meio de cultura suplementado com 0,2 mg.l -1 2,4-D e 2 mg.l -1 BAP e sem carvão ativado, resultaram em maior crescimento (massa fresca) dos calos. A partir destes calos, foram obtidas suspensões celulares, cuja dinâmica de crescimento e acúmulo de metabólitos foi estudado. Os resultados obtidos deverão ser utilizados para a caracterização de rotas biosintéticas em culturas in vitro em P. solmsianum. Palavras-chave: calogênese, morfogênese, pariparoba, reguladores de crescimento, suspensões celulares ABSTRACT -(In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)).Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l -1 ) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l -1 2,4-D and 2 mg.l -1 BA supplemented medium, and in the absence of activated charcoal, showed the highest calli fresh matter increment. From a callus culture, cell suspension cultures were established and their growth and metabolite accumulation studied. The achieved results may be useful for further characterization of the activated secondary metabolites pathways in in vitro systems of P. solmsianum.

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Section: Resultsmentioning

confidence: 99%

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

et al. 2009

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“…leaves (Texeira et al 1993;Texeira et al 1994). The frequencies for complete plant regeneration from some explants are still inefficient (Rival et al 1999).…”

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confidence: 99%

Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

Abdullah¹,

Zainal²,

Heng³

et al. 2005

Electro Journal of Biotech

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“…This may be because these somatic embryos are in contact with auxin from the beginning of callus induction and the roots develop after having absorbed a sufficient amount of auxin from the media of previous stages. Teixeira et al (1994) also observed the regeneration and conversion of SEs in palm plants on MS medium devoid of growth regulators.…”

Section: Discussionmentioning

confidence: 85%

Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)

Bonetti¹,

Nesi²,

Quisen³

et al. 2016

Afr. J. Biotechnol.

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Oil palm hybrid BRS Manicoré is important for plantations in the north of Brazil, as it is resistant to fatal yellowing and is compact. Seed germination is slow and reduced, so somatic embryogenesis is a promising alternative for its propagation. Two kinds of starting explants were used: Zygotic embryos (ZE) and thin cell layers (TCL) from the base of seedling aerial parts. Two culture medium formulae were compared (Y3 and modified MS), and several growth regulators (2,4-D, Picloram, BA, 2iP), putrescine and activated charcoal (AC) were used during the different stages. Somatic embryos (SE) were obtained from ZE-derived embryogenic masses cultured on Y3 medium with or without 2,4-D (9 µM) + 1000 µM putrescine and 40% were converted into seedlings in the Y3 medium supplemented with 2 g.L -¹ AC and without growth regulators, under light. SE developed in 49 to 53% TCL-derived calli and 50% of them were converted into seedlings in the same conditions described for ZE calli. The yield of both processes was compared, showing the superiority of TCL explants for SE production.

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Somatic embryogenesis from immature inflorescences of oil palm

Cited by 92 publications

References 8 publications

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)

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