Somatic embryogenesis in cassava genotypes from the northeast of Brazil

Feitosa, Terezinha; Bastos, João Luíz Pinheiro; Ponte, Luiz Ferreira Aguiar; Jucá, Thiago Lustosa; Campos, Francisco

doi:10.1590/s1516-89132007000200004

Cited by 21 publications

(28 citation statements)

References 10 publications

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“…Table 2 demonstrates the influence of different concentrations of picloram in MS medium supplemented with 0.318 mg/l copper sulfate for the induction of calli from axillary bud explants. Copper sulfate has been reported to induce embryogenic calli in several crops such as cassava and sorghum (Nirwan and Kothari 2003;Feitosa et al 2007).…”

Section: Effect Of Different Concentrations Of Picloram Supplemented mentioning

confidence: 99%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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Dioscorea rotundata, commonly known as white yam, is an important staple food crop widely cultivated in West Africa and provides food security to millions of people. Genetic improvements of this crop using the advanced biotechnology tools have been hampered hitherto by the recalcitrant nature of regeneration through somatic embryogenesis. Therefore, we have developed an efficient and reproducible system for plant regeneration via somatic embryogenesis. Explants of different types (immature leaf, node, stem internode, root segment, petiole, and axillary bud) of D. rotundata accession TDr 2436 were tested for their embryogenic potentials on Murashige and Skoog (MS) medium supplemented with various auxins (2,4-D, NAA, and picloram). Among all explants tested, axillary bud explants cultured on MS medium supplemented with picloram (0.5-12 mg/l) favored the production of calli. Maximum proliferation of calli (526 mg fresh weight/explant) was achieved on MS medium supplemented with picloram (0.5 mg/l), casein hydrolysate (600 mg/l), and proline (1 g/l). Histology analysis confirmed that the embryogenic calli produced on this medium were mixed with non-embryogenic calli. Transfer of calli on MS basal medium supplemented with activated charcoal (1 %) changed the color of calli to purple and promoted the production of somatic embryos (87 embryos/callus) as well as adventitious shoot buds. Furthermore, upon transfer to MS medium supplemented with BAP (0.4 mg/l), the embryos continued their differentiation and maturation and germinated into complete plantlets. The adventitious shoot buds produced multiple shoots on MS medium supplemented with BAP (0.4 mg/l). Well-developed germinated plantlets were acclimatized in the screen house with 90 % survivability. Histology studies confirmed that the regeneration of D. rotundata reported here followed dual regeneration pathways. The embryogenic calli regenerated through development of somatic embryos and germinated into complete plantlets, however non-embryogenic calli regenerated through organogenesis and developed multiple shoots. The developed protocol has potential for somatic hybridization, mass clonal propagation, and genetic transformation applications.

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Section: Effect Of Different Concentrations Of Picloram Supplemented mentioning

confidence: 99%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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“…In this work, the callus of Manihot esculenta was induced from budded nodal cuttings in the presence of NAA. Many works have been carried out on the establishment of callus culture from cassava using different explants (Peter et al, 2011;Fietosa et al, 2007;Atehnkeng et al, 2005;Schopke et al, 1996), but this is the first time that callus is established from budded nodal cutting of this species. The highest percentage of callogenesis (59.8%) was obtained with 0.08 mg.L -1 NAA.…”

Section: Effect Of Naa On the Production And Growth Of Callus From Bumentioning

confidence: 99%

Effect of exogenous phytohormones and sucrose on micropropagation and microtuberisation of Manihot esculenta Crantz var. TMS 96/0023

Fotso¹,

Elian²,

Tchouga³

et al. 2014

Afr. J. Biotechnol.

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“…Many works have been carried out on the establishment of callus culture from cassava in different laboratories (Fietosa et al, 2007;Atehnkeng et al, 2005, Schopke et al, 1996. But this is the first time one is done on Ghanaian cassava varieties.…”

Section: Discussionmentioning

confidence: 99%

Effect of 2,4-D, explants type and cultivar on the callogenesis expression of cassava (Manihot esculenta Crantz) in Ghana

Fletcher¹,

Amoako²,

Twumasi³

2011

Afr. J. Biotechnol.

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Modern biotechnological applications in breeding programmes for economically-important crops like cassava begins with successful establishment of stable and active callus cultures for somatic embryos which can be genetically manipulated and induced into planting materials. We have investigated the effects of explants (leaves, stem petiole and axillary buds), auxin (2, 4-D) concentrations (8, 12 and 15 mg/l) and cultivar in the induction of callus cultures from local cassava varieties in Ghana -Tuaka, Afisiafi, Afebankye and Doku. Callus formation was successfully induced in all explants (leaf, petiole and auxiliary buds) from the four cassava cultivars. Generally, there were no significant differences in callogenesis of the different explants at the three different 2,4-D concentrations (p < 0.05) producing 75% callus for explants. The stem petiole or stem bark produced the least percentage of callus per cultivar at all the auxin concentrations. However, the leaf explants were superior in callus formation in all the cassava varieties. Although, statistically insignificant, the 8 mg/l 2,4-D concentration was visually the best in callus formation from explants both within and across the cassava cultivars. This study had shown that different cassava explants respond differently to tissue culture conditions established for callus culture formation.

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Somatic embryogenesis in cassava genotypes from the northeast of Brazil

Cited by 21 publications

References 10 publications

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Effect of exogenous phytohormones and sucrose on micropropagation and microtuberisation of Manihot esculenta Crantz var. TMS 96/0023

Effect of 2,4-D, explants type and cultivar on the callogenesis expression of cassava (Manihot esculenta Crantz) in Ghana

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