Somatic Embryogenesis in Two Orchid Genera (Cymbidium, Dendrobium)

Silva, Jaime A. Teixeira da; Winarto, Budi

doi:10.1007/978-1-4939-3061-6_18

Cited by 16 publications

(6 citation statements)

References 27 publications

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“…In this medium, periodic subcultures (4-5 times, each subculture period was 15 days) were carried out in the same medium to proliferate PLBs. For more details, also see Teixeira da Silva et al (2015a) and Teixeira da Silva and Winarto (2016). Fourth, bacterial (I-O) and fungal (P-U) contamination of Dendrobium 'Zahra FR 62' (I, J, L, M, O, R), D. 'Gradita 31' (K, N, P, S) and D. 'Jayakarta' (Q, T) in solid and semi-solid cultures of shoot tips (I-K) or PLBs (L-T) can take place 2 months (I-Q) or 1.5 months (R-T) after culture initiation on half-strength MS containing 0.3 mg dm -3 TDZ and 0.1 mg dm -3 NAA.…”

Section: Choice Of Methods For Surface Disinfectionmentioning

confidence: 99%

Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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Establishing an aseptic in vitro culture for Dendrobium, or for any plant in fact, is the most important step towards developing an effective in vitro tissue culture including micropropagation protocol. Success in initial aseptic culture will contribute to the successful production of in vitro cultures that may involve the initiation or formation of callus and/or protocorm-like bodies (PLBs), the induction, regeneration or multiplication of shoots, and the preparation and proliferation of plantlets suitable for acclimatization. The initiation of an aseptic culture is closely related to the appropriate selection of an explant source and its preparation, including its (in vivo) pre-treatment if necessary and subsequent disinfection procedures. Care in the choice of explant and the application of an appropriate disinfection protocol can successfully reduce, or eliminate, contamination in in vitro cultures while reducing the negative impact on plant tissues and plantlet regeneration. Many unique aseptic culture procedures for Dendrobium genus have been reported in the literature, very often specific to particular tissues or genotypes, and this review not only highlights the details of such protocols, but also provides practical advice for novice -and even seasoned -orchidologists who wish to research Dendrobium in vitro, although it is cautioned that there is currently no universal aseptic culture procedure that can be applied to all conditions, all explants or all genotypes.

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Section: Choice Of Methods For Surface Disinfectionmentioning

confidence: 99%

Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

Self Cite

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“…Besides the influence of light, another admittedly important factor in the induction of PLBs in Phalaenopsis and orchids in general is the genotype [61]. This means that under the same cultivation condition, the induction responses of PLBs may be significantly different [62], which is still considered a limitation of the technique.…”

Section: Genus Phalaenopsis and Relatedmentioning

confidence: 99%

“…Regarding the type of explant, leaf segments of plants grown in vitro have been the most suitable for induction of PLBs in Phalaenopsis (45% of papers; Table 1), but there are reports of protocols that Besides the influence of light, another admittedly important factor in the induction of PLBs in Phalaenopsis and orchids in general is the genotype [61]. This means that under the same cultivation condition, the induction responses of PLBs may be significantly different [62], which is still considered a limitation of the technique.…”

Section: Genus Phalaenopsis and Relatedmentioning

confidence: 99%

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Cardoso

Zanello

Chen

2020

IJMS

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The process through induction, proliferation and regeneration of protocorm-like bodies (PLBs) is one of the most advantageous methods for mass propagation of orchids which applied to the world floricultural market. In addition, this method has been used as a tool to identify genes of interest associated with the production of PLBs, and also in breeding techniques that use biotechnology to produce new cultivars, such as to obtain transgenic plants. Most of the molecular studies developed have used model plants as species of Phalaenopsis, and interestingly, despite similarities to somatic embryogenesis, some molecular differences do not yet allow to characterize that PLB induction is in fact a type of somatic embryogenesis. Despite the importance of species for conservation and collection purposes, the flower market is supported by hybrid cultivars, usually polyploid, which makes more detailed molecular evaluations difficult. Studies on the effect of plant growth regulators on induction, proliferation, and regeneration of PLBs are the most numerous. However, studies of other factors and new technologies affecting PLB production such as the use of temporary immersion bioreactors and the use of lighting-emitting diodes have emerged as new tools for advancing the technique with increasing PLB production efficiency. In addition, recent studies on Phalaenopsis equestris genome sequencing have enabled more detailed molecular studies and the molecular characterization of plantlets obtained from this technique currently allow the technique to be evaluated in a more comprehensive way regarding its real applications and main limitations aiming at mass propagation, such as somaclonal variation.

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“…There are two pathways in somatic embryogenesis. First, single-cellderived somatic embryo is generated, develops into a globular somatic embryo similar to zygotic embryogenesis (27) which later become PLBs with shoot tip, and eventually develops into plantlets (10).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…The other is the production of multiple-cell-derived somatic embryos that directly form globular or multicellular somatic embryos, lacking the typical early stages of embryogenesis (27).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Efficient micropropagation of Dendrobium aurantiacum from shoot explant

Ling¹,

Khoo²,

Lee³

et al. 2020

Plant Sci. Today

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Embryogenic tissue culture (seed tissue culture) is a common practice in plant industry to speed up and mass production. However, the culture method is not widely adopted in most of the orchid species. Micro-size orchid seeds are difficult to obtain and collect due to ambiguous seed maturation period. Most of orchid seeds have no endosperm and highly dependent on the specific fungi for germination and survival. Micropropagation from shoot culture with meristem tissue is potentially be another alternative for mass propagation of orchid. Therefore, this study examine the potential of micropropagation technique by shoot culture in orchid, Dendrobium aurantiacum (F. Muell.) F. Muell. This study reported an effective aseptic technique to develop sterilized D. aurantiacum tissue in vitro. The callus induction and regeneration from shoot explant by utilization of different plant growth regulator had been examined in this study. Among the treatments, 20% sodium hypochlorite with 15 mins sterilization period showed the highest sterilization efficiency on explants with only 16.7±5.8% of contamination occurred after two weeks and obtained highest survival rate 73.3±5.8% after one month. Callus formed in all combinations of plant hormone treatments. Media treated with 10 mg/L 2,4-D showed the highest callus induction rate but browning condition occur after 3 months of culture. Cell count on callus proliferation showed a significant difference (p < 0.05) between control and treatments. In conclusion, micropropagation of D. aurantiacum had been shortened almost 9-12 months required for nature germination.

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Somatic Embryogenesis in Two Orchid Genera (Cymbidium, Dendrobium)

Cited by 16 publications

References 27 publications

Tissue disinfection for preparation of Dendrobium in vitro culture

Tissue disinfection for preparation of Dendrobium in vitro culture

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Efficient micropropagation of Dendrobium aurantiacum from shoot explant

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