Somatic Embryogenesis in Wheat and Barley Calli in vitro Is Determined by the Level of Indoleacetic and Abscisic Acids

Seldimirova, O.A.; Kudoyarova, G. R.; Круглова, Н.Н.; Galin, Ilshat; Веселов, Д. С.

doi:10.1134/s1062360419030056

Cited by 14 publications

(15 citation statements)

References 49 publications

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“…This has been revealed, for example, in some cereals [12,14,19]. So, for wheat and barley, it was found that the ability to somatic embryogenesis in vitro was determined by the ratio of IAA:ABA content in calli, with rather similar cytokinins content in both embryogenic and non-embryogenic calli types [14]. In our work, it was shown that in the morphogenic lavender calli, the content of cytokinins (zeatin riboside and zeatin nucleotide) increased and the content of ABA and IAA decreased.…”

Section: Resultsmentioning

confidence: 85%

“…The ability of calli to develop along the certain path of morphogenesis is largely due to the balance between the content of endogenous hormones in the callus and the concentration of exogenous hormones in the culture medium. This has been revealed, for example, in some cereals [12,14,19]. So, for wheat and barley, it was found that the ability to somatic embryogenesis in vitro was determined by the ratio of IAA:ABA content in calli, with rather similar cytokinins content in both embryogenic and non-embryogenic calli types [14].…”

Section: Resultsmentioning

confidence: 93%

“…Calli were homogenized, extracted with 80% ethanol and incubated at 4°C during 12 h. The homogenate was filtered; the filtrate was evaporated to aqueous residues. The quantitative content of hormones was tested by the enzyme immunoassay method adapted by authors to the calli study [14].…”

Section: Methodsmentioning

confidence: 99%

“…One of the directions in the development of plant biotechnologies using calli is the identification of the relationship between the content of endogenous (in explant or callus) and the concentration of exogenous (in a culture medium) hormones for regulating morphogenesis pathways in vitro. Such studies are not numerous and they were carried mainly on cereals [12][13][14]. Based on the available literature, studies of endogenous hormones accumulation in order to identify their participation in the callusogenesis and the regulation of morphogenesis in callus cultures in vitro for lavender have not been previously conducted.…”

Section: Introductionmentioning

confidence: 99%

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Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.

et al. 2020

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The influence of the nutrient medium hormonal composition on the induction of calluso- and morphogenesis in Lavandula angustifolia Mill., as well as the content of some endogenous hormones (indolylacetic acid, cytokinins, abscisic acid) in morphogenic and non-morphogenic calli, were studied. The leaf explants were isolated from obtained in vitro plants of the Stepnaya and Vdala cultivars. In primary calli with a frequency of 5.7-11.6%, buds and shoots were formed on different Murashige and Skoog (MS) culture media supplemented with 6-benzylaminopurine, thidiazuron, kinetin, and gibberellic acid. In the callus of the first passage in both cultivars, the induction of morphogenesis was observed with the greatest frequency (39.5-43.2%) on the MS medium with the addition of 1.0 mg/l 6-benzylaminopurine and 1.0 mg/l kinetin. Morphogenic calli of the Vdala cultivar were characterized by a higher content of cytokinins, but a lower content of indolylacetic and abscisic acids compared with non-morphogenic ones.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.

et al. 2020

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“…прошлого века [5]. Анализ результатов многолетних экспериментальных исследований [6][7][8][9][10][11] позволил авторам сделать ряд теоретических обобщений [2,12] и предложить оценивать каллус in vitro как модельную систему для изучения сложнейшей проблемы морфогенеза растений [13,14].…”

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Callus culture in vitro in the experimental evaluation of drought resistance in cereals (review)

Круглова¹,

Seldimirova²,

Zinatullina³

2021

TVAN

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One of the directions in plant biotechnological research is the use of callus cultures in vitro. The callus is an integrated system that is formed both exogenously (as a result of the proliferation of surface cells of various tissues of the plant organism) and endogenously (in the depth of these tissues). Initially, callus consists of homogeneous cells gradually transformed into a system of groups of heterogeneous cells with specific morphogenetic potencies. These potencies are realized by various ways of morphogenesis in vitro, including those leading to the formation of full-fledged plants-regenerants. In this review, using cereals as an example, we analyze the literature and our data on the use of calli in vitro as experimental systems for studying the plant stress-resistance to abiotic factors, especially to the drought factor. The advantages and limitations of using callus cultures in vitro are considered. The prospects of studying the mechanisms of action of abiotic stressors and resistance to them at the cellular and tissue levels under model conditions of callus culture in vitro are shown. Attention is paid to the assessment of the drought resistance of cereals under selective conditions simulating drought in vitro by such a parameter as the growth activity of calli. The issue of studying anti-stress effects in callus cultures in vitro is analyzed on the example of brassinosteroids. The prospects of using the model system “immature embryo in vivo–embryonic morphogenic callus in vitro” for rapid assessment of the effect of anti-stress plant growth regulators are considered. It is emphasized that the basis for using calli as the model system is both the significant role of the cell in all morphogenetic events of the plant organism in vivo and in vitro and the similarity of the responses of plants in vivo, calli in vitro, regenerants of callus origin in vitro and ex vitro.

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Inorganic Compounds that Aid in Obtaining Somatic Embryos

Atanacio-López

Luna-Rodríguez

Soto-Contreras

et al. 2022

Methods in Molecular Biology

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Somatic Embryogenesis in Wheat and Barley Calli in vitro Is Determined by the Level of Indoleacetic and Abscisic Acids

Cited by 14 publications

References 49 publications

Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.

Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.

Callus culture in vitro in the experimental evaluation of drought resistance in cereals (review)

Inorganic Compounds that Aid in Obtaining Somatic Embryos

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