Some epidemiological studies on brucellosis in dairy farms in Gharbia governorate .Egypt.

Abstract

This study was conducted on dairy farms in Gharbia governorate, Egypt from January to December 2018. 240 dairy farms were included, and 3775 serum sample were collected for serological tests. 25 farms (10.42) were positive for brucellosis, 132 serum sample were positive by serological test classified according to the productive stage of animals as 121/3000 (4.033%), 8/600 (1.33%) and 2/175 (1.142%) in cows, heifers and bulls, respectively. The culturing and PCR confirmation results indicated that Brucella melitensis biovar 3 was the dominant strain in the tested dairy farms. The spatial distribution of positive cases indicated that districts of Samnood, Kfr-Elzyat and Basion had the highest rate of positive cases (6.35%, 4.67% and 3.57%, respectively), yet El-Santa, Zefta and El-Mahla El-Kobra districts showed the lowest rates (1.49%, 2.3% and 2.89%, respectively). The obtained result proved that brucellosis is endemic in Gharbia governorate and good control program should be conducted to eradicate the disease.

Conventional, Serological and Molecular Characterization of Brucella Species Isolated from Different Governorates in Egypt

“…Results of culturing of tissue samples from lymph nodes, spleen, liver, aborted foeti and milk were 13.7 %, 0.034 %, 0%, 25 % and 5.4% respectively table (6). These findings come in accordance with Aman et al et al (2020) 5 out of 200 (with an incidence rate of 2.5%) milk samples were positive for Brucella and grow on Brucella specific media On the other hand, a higher rate of isolation of Brucella organism reported by Khalafallah et al (2020) Results of culturing of tissue samples from lymph nodes, spleen and liver were 61.54%, 40.38% and 36.54% respectively H.I. Hosein et al (2017) 104 cows and 46 milk samples of sero-positive cows revealed isolation of 64 (61.5%) and 28 (60.9) brucella isolates respectively that were identified as Brucella melitensis biovar 3.…”

“…melitensis DNA. The obtained results were agreed with that reported previously by Ilhan et al (2008) and El-Shymaa (2014)., Wareth et al (2015), who reported that PCR must be considered an alternative to the traditional culturing methods for Brucella diagnosis as screening and confirmatory diagnostic tool for saving cost and time, Also these results were similar to that obtained by Lobna M.A.et al, 2016, Khalafallah et al (2020.…”

“…Accordingly, we employed RBPT for brucellosis screening and then confirmed the results by STAT; this combination was used to minimize measurement errors of false positives (Franco MP et al 2007, Tumwine G et al 2015. Result of MSTAT in our study is 80.2% table (4) higher than recorded by (Ahmed et al, 2016) (8.9 %), (Khalafallah et al 2020) (3.47 %) and lower than recorded by AL-Habaty et al, 2015 (SAT 90.9 %). Serological methods, most commonly STA, are frequently used in the diagnosis of brucellosis (Gul HC et al 2016).…”