Some miRNA expressions and their targets in ischemic stroke

Can, Ummugulsum; Marzioglu, Ebru; Akdu, Sadinaz

doi:10.1080/15257770.2022.2098974

Cited by 4 publications

(2 citation statements)

References 165 publications

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“…Further studies found that PRDX1 had the strongest positive correlation with type 2T helper cells, consistent with previous studies 73,74 , and GPX4 had the strongest negative correlation with neutrophils, consistent with previous studies 75,76 . miRNAs play an important role in IS development 77 . In this study, 3747 miRNA-mRNA-TF regulatory pairs were identified in the miRNA-mRNA-TF regulatory network, including hsa-miR-4469-CDKN1A-BACH2 and hsa-miR-188-3p-GPX4-ATF2.…”

Section: Discussionmentioning

confidence: 99%

Analysis and identification of oxidative stress-ferroptosis related biomarkers in ischemic stroke

Zhang,

Liang,

Xiong

et al. 2024

Sci Rep

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Studies have shown that a series of molecular events caused by oxidative stress is associated with ferroptosis and oxidation after ischemic stroke (IS). Differential analysis was performed to identify differentially expressed mRNA (DEmRNAs) between IS and control groups. Critical module genes were identified using weighted gene co-expression network analysis (WGCNA). DEmRNAs, critical module genes, oxidative stress-related genes (ORGs), and ferroptosis-related genes (FRGs) were crossed to screen for intersection mRNAs. Candidate mRNAs were screened based on the protein–protein interaction (PPI) network and the MCODE plug-in. Biomarkers were identified based on two types of machine learning algorithms, and the intersection was obtained. Functional items and related pathways of the biomarkers were identified using gene set enrichment analysis (GSEA). Finally, single-sample GSEA (ssGSEA) and Wilcoxon tests were used to identify differential immune cells. An miRNA-mRNA-TF network was created. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the expression levels of biomarkers in the IS and control groups. There were 8287 DE mRNAs between the IS and control groups. The genes in the turquoise module were selected as critical module genes for IS. Thirty intersecting mRNAs were screened for overlaps. Seventeen candidate mRNAs were also identified. Four biomarkers (CDKN1A, GPX4, PRDX1, and PRDX6) were identified using two types of machine-learning algorithms. GSEA results indicated that the biomarkers were associated with steroid biosynthesis. Nine types of immune cells (activated B cells and neutrophils) were markedly different between the IS and control groups. We identified 3747 miRNA-mRNA-TF regulatory pairs in the miRNA-mRNA-TF regulatory network, including hsa-miR-4469-CDKN1A-BACH2 and hsa-miR-188-3p-GPX4-ATF2. CDKN1A, PRDX1, and PRDX6 were upregulated in IS samples compared with control samples. This study suggests that four biomarkers (CDKN1A, GPX4, PRDX1, and PRDX6) are significantly associated with IS. This study provides a new reference for the diagnosis and treatment of IS.

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Section: Discussionmentioning

confidence: 99%

Analysis and identification of oxidative stress-ferroptosis related biomarkers in ischemic stroke

Zhang,

Liang,

Xiong

et al. 2024

Sci Rep

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“…MicroRNA (miRNA) is a noncoding RNA with a size of about 20-25 nt, which plays a regulatory function by interacting with target mRNA (An et al, 2023;Anglicheau et al, 2010;Sacar Demirci et al, 2019;Wang et al, 2023). Abnormal miRNA expression may lead to the occurrence and development of human diseases, including IS (Can et al, 2022;Gugliandolo et al, 2021;He et al, 2023;Vazquez et al, 2023). Many miRNAs have been confirmed to be related to IS process, such as miR-451 (Wang et al, 2021) and miR-641 (Pan et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Baicalin alleviates oxygen–glucose deprivation/reoxygenation‐induced SK‐N‐SH cell injury via the regulation of miR‐556‐3p/ACSL4 pathway

Dai,

Yue,

Zhang

et al. 2024

Chem Biol Drug Des

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Baicalin (BCL) has been found to have neuroprotective effects in ischemic stroke (IS), but its underlying molecular mechanisms are unknown. SK‐N‐SH cells were treated with BCL and then induced by oxygen–glucose deprivation/reoxygenation (OGD/R). Cell proliferation, apoptosis, inflammation, and ferroptosis were detected. Protein levels were examined by western blot. The expression levels of microRNA (miR)‐556‐3p and ACSL4 were tested via quantitative real‐time PCR. MiR‐556‐3p and Acyl‐CoA synthetase long‐chain family member 4 (ACSL4) interaction was confirmed via dual‐luciferase reporter assay and RNA pull‐down assay. Middle cerebral artery occlusion (MCAO) mice model was constructed to assess the role of BCL on brain injury in vivo. Our study showed that BCL treatment alleviated OGD/R‐induced SK‐N‐SH cell apoptosis, inflammation and ferroptosis. MiR‐556‐3p was decreased in OGD/R‐induced SK‐N‐SH cells, and BCL treatment enhanced its expression. MiR‐556‐3p could target ACSL4, and its overexpression relieved OGD/R‐induced SK‐N‐SH cell injury by targeting ACSL4. Besides, miR‐556‐3p inhibitor or ACSL4 overexpression reversed the inhibitory effect of BCL on OGD/R‐induced SK‐N‐SH cell injury. In vivo, BCL alleviated brain injury in MCAO mice through miR‐556‐3p/ACSL4 axis. In conclusion, BCL alleviated OGD/R‐induced SK‐N‐SH cell injury and relieved brain injury in MCAO mice by regulating miR‐556‐3p/ACSL4 axis.

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