Some Studies on Red Blood Cells Morphology of Healthy and Diabetic Patients in Taiz, Yemen

Abstract

The aim of this study was to: 1- Identify and quantify the prevalence of RBC abnormalities in healthy and diabetic subjects. 2- Provide supporting evidence about the relation between RBC storage duration at 4oC and alterations to RBC morphology (compare with the morphology at the time of collection). 3- The obtain information about how the number of normal cells in different times of storage declines as a function of the storage period. 4- Estimate the prevalence of red cell morphological changes in diabetic patients.
 One hundred and ninety-six slides of 49 healthy and 49 diabetic patients of different age groups were collected from November 2019 to March 2020.
 Human venous blood samples were taken and anticoagulated with EDTA. samples were divided into 4 groups, group 1 was examined at once, and groups 2-4 were stored at 4oC for 24, 48, and 72 hours respectively.
 During the current study, abnormalities of erythrocyte morphology, prevalence, and histological effects of storage duration on the human blood cells were evaluated. 16 different types of abnormality in shapes of the red blood cells were identified in healthy subjects and 19 different shapes in diabetic subjects, with the difference in the prevalence percentage. Analysis of variance (ANOVA) exhibited statistically significant effects of storage time (24, 48, and 72 hours at 40C) on RBC morphology. The present result also shows that the change in erythrocyte shapes at once beginning and during time storage were statistically significant between healthy and diabetic donors. These results are in line with previous laboratory studies on other parameters.
 In conclusion, our observations indicate that morphological abnormalities of erythrocytes are common in healthy and diabetic subjects, and the slight effects of diabetic Mellitus on the changes observed in erythrocyte compare to healthy subjects over 72 hours of storage.