Sonic hedgehog in oral squamous cell carcinoma: An immunohistochemical study

Abstract

Background:Recent studies have revealed the involvement of hedgehog (Hh) signaling component in proliferation and invasive behavior of many carcinomas.Aim:This study aims to identify the expression of sonic Hh (SHH) protein of SHH pathway in oral epithelial dysplasia and oral squamous cell carcinoma (OSCC) using SHH (H-160) (Santa Cruz, sc-9042) which could have therapeutic implication in future.Materials and Methods:A total of 250 cases comprising 50 normal oral mucosa, 50 cases of oral epithelial dysplasia, 50 well, 50 moderate and 50 poorly differentiated OSCCs were included in the study. Immunohistochemical evaluation of SHH protein expression was conducted using monoclonal antibody. Interpretation of the expression was done by immunoreactive score of Remmele and Stegner (IRS) scoring method.Statistical Analysis:Chi-Square test was used to analyze the results.Results:The study showed that SHH signaling molecules are highly expressed in OSCC, and their expression was mainly in the cytoplasm of epithelial cells.Conclusion:The SHH signaling component is associated with the pathological parameter in OSCC and oral epithelial dysplasia.

Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

“…Three Hedgehog homologs are present in mammals: Desert (DHH), Indian (IHH) and Sonic (SHH) 160 . Immunohistochemical results show that SHH signaling molecules are highly expressed in OSCC and considered as a potential target in oral cancer therapy 161,162 . In addition, SHH signaling is a potential promotor of OSCC associated with bone destruction 163,164 .…”

TRAIL, Wnt, Sonic Hedgehog, TGFβ, and miRNA Signalings Are Potential Targets for Oral Cancer Therapy

“…Recently, several SHH-signaling proteins were also found overexpressed in OSCC patients. For example, SHH was highly expressed in OSCC and appeared in the cytoplasm of epithelial cells in immunohistochemical analyses [ 59 ]. Both SHH and GLI family zinc finger 1 (glioma-associated oncogene homolog 1; GLI1) are notably upregulated in 74 OSCC samples of immunohistochemical analyses.…”

Multifactorial Contribution of Notch Signaling in Head and Neck Squamous Cell Carcinoma

“…Mutations in HH-pathways have been found in basal cell carcinoma, medulloblastoma, and rhabdomyosarcoma, and normally correlate with an hyperactivation of the pathway. Recent studies on protein expression showed that SHH signaling components are highly expressed in OSCC compared to normal tissue, with a restricted positive pattern in epithelial cells [160,161,162,163].…”