Sonication-induced unfolding proteins

Stepanskiy, Leonard G.

doi:10.1016/j.jtbi.2012.01.008

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…171,172 Sonication affects protein aggregation and causes partial unfolding due to an increase in temperature, pressure, and void formation. 173,174 Additionally, the long-term storage of proteincontaining syringes could produce particles and aggregates of protein due to the formation of radicals on exposure to radiation during the sterilization process. 175…”

Section: Processesmentioning

confidence: 99%

Review of the current state of protein aggregation inhibition from a materials chemistry perspective: special focus on polymeric materials

et al. 2021

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Section: Processesmentioning

confidence: 99%

Review of the current state of protein aggregation inhibition from a materials chemistry perspective: special focus on polymeric materials

et al. 2021

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“…Here, we focus on the smallest possible unfolding system - a closed chain of carbon atoms - to investigate the limits of stability at the atomistic scale. Insights from such structures can then be applied to more complex macromolecular systems, such as responsive polymer [5,6] or protein-based materials [7-10]. …”

Section: Introductionmentioning

confidence: 99%

Thermal stability of idealized folded carbyne loops

Cranford

2013

Nanoscale Res Lett

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Self-unfolding items provide a practical convenience, wherein ring-like frames are contorted into a state of equilibrium and subsequently pop up’ or deploy when perturbed from a folded structure. Can the same process be exploited at the molecular scale? At the limiting scale is a closed chain of single atoms, used here to investigate the limits of stability of such folded ring structures via full atomistic molecular dynamics. Carbyne is a one-dimensional carbon allotrope composed of sp-hybridized carbon atoms. Here, we explore the stability of idealized carbyne loops as a function of chain length, curvature, and temperature, and delineate an effective phase diagram between folded and unfolded states. We find that while overall curvature is reduced, in addition to torsional and self-adhesive energy barriers, a local increase in curvature results in the largest impedance to unfolding.

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“…BEAS-2B cells were seeded overnight in a 12-well plate (Fisher) at 2.5 × 10 5 cell/well and exposed to 50 μg/ml Alexa-BSA-SWCNT conjugates dispersed in cellular media by brief sonication (intervals of 10 s were used to avoid loss of protein functionality (Stepanskiy, 2012) or reduction of the fluorophore intensity) (Welsher et al, 2009). Controls (i.e., unexposed cells, cells exposed to unlabeled SWCNTs, and cells exposed to free Alexa-BSA at equivalent amounts to the amount of protein loaded onto the samples) were performed in parallel.…”

Section: Methodsmentioning

confidence: 99%

Carbon nanotubes physicochemical properties influence the overall cellular behavior and fate

et al. 2018

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The unique properties of single walled carbon nanotubes (SWCNTs) make them viable candidates for versatile implementation in the next generation of biomedical devices for targeted delivery of chemotherapeutic agents or cellular-sensing probes. Such implementation requires user-tailored changes in SWCNT’s physicochemical characteristics to allow for efficient cellular integration while maintaining nanotubes’ functionality. However, isolated reports showed that user-tailoring could induce deleterious effects in exposed cells, from decrease in cellular proliferation, to changes in cellular adhesion, generation of reactive oxygen species or phenotypical variations, just to name a few. Before full implementation of SWCNTs is achieved, their toxicological profiles need to be mechanistically correlated with their physicochemical properties to determine how the induced cellular fate is related to the exposure conditions or samples’ characteristics. Our study provides a comprehensive analysis of the synergistic cyto- and genotoxic effects resulted from short-term exposure of human lung epithelial cells to pristine (as manufactured) and user-tailored SWCNTs, as a function of their physicochemical properties. Specifically, through a systematic approach we are correlating the nanotube uptake and nanotube-induced cellular changes to the sample’s physicochemical characteristics (e.g., metal impurities, length, agglomerate size, surface area, dispersion, and surface functionalization). By identifying changes in active hallmarks involved in cell-cell connections and maintaining epithelial layer integrity, we also determine the role that short-term exposure to SWCNTs plays in the overall cellular fate and cellular transformation. Lastly, we assess cellular structure-function relationships to identify non-apoptotic pathways induced by SWCNTs exposure that could however lead to changes in cellular behavior and cellular transformation. Our results show that the degree of cell transformation is a function of the physicochemical properties of the SWCNT, with the nanotube with higher length, higher metal content and larger agglomerate size reducing cell viability to a larger extent. Such changes in cell viability are also complemented by changes in cell structure, cycle and cell-cell interactions, all responsible for maintaining cell fate.

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Sonication-induced unfolding proteins

Cited by 5 publications

References 26 publications

Review of the current state of protein aggregation inhibition from a materials chemistry perspective: special focus on polymeric materials

Review of the current state of protein aggregation inhibition from a materials chemistry perspective: special focus on polymeric materials

Thermal stability of idealized folded carbyne loops

Carbon nanotubes physicochemical properties influence the overall cellular behavior and fate

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