“Sorbose Toxicity” in Neurospora

Abstract

Brockman, II. E., and F. J. de Serres. (Oak Ridge Natl. Lab., Oak Ridge, Tenn.) “Sorbose toxicity” in Neurospora. Amer. Jour. Bot. 50(7): 709–714. Illus. 1963.—The effect of “sorbose toxicity” on Neurospora conidia or ascospores was compared in sorbose‐fructose‐glucose (S‐F‐G) and sorbose‐sucrose (S‐S) media. Many frequently encountered and difficultly controlled experimental variables may strongly affect viability in S‐S media but are essentially without effect in S‐F‐G media. The viabilities of different mutant strains are affected to varying extents by autoclave exposure time of the S‐S media but not of the S‐F‐G media. Ascospores are more sensitive than conidia to “sorbose kill” in S‐S media. An over plating method described by New meyer (1954) for increasing ascospore “viability” is without effect when sucrose is replaced with fructose and glucose. In all experiments in which sorbose was added to induce colonial growth, “sorbose toxicity” or “sorbose kill” was eliminated or minimized by replacing sucrose with a mixture of fructose and glucose as the carbon source for Neurospora media.

Cyclosporin A-resistance based gene placement system for Neurospora crassa

“…Growth, preparation of mycelia, genetic techniques, and other routine manipulations were performed as described by Davis and de Serres (1970). A sorbose-glucose-fructose medium was used for restrictive colonial growth (Brockman and de Serres, 1963).…”

Genes encoding a striatin-like protein (ham-3) and a forkhead associated protein (ham-4) are required for hyphal fusion in Neurospora crassa

“…Strains were grown on Vogel's minimal medium (Vogel, 1956) with required supplements. BDES medium was used to induce colonial growth (Brockman and de Serres, 1963). Crosses were performed on Westergaards's medium (Westergaard and Mitchell, 1947).…”

A mutation in the Neurospora crassa actin gene results in multiple defects in tip growth and branching

“…To screen for cytochalasin A resistance, a suspension of 1 Â 10 7 wild type A conidia was irradiated with UV rays for 1 min and plated on sorbose-containing medium (Brockman and de Serres, 1963) with 7 lg/ml (14.66 lM) cytochalasin A. The potency of cytochalasin A (SigmaAldrich Canada, Oakville, ON) varied somewhat between different lots.…”