Sorghum’s Whole-Plant Transcriptome and Proteome Responses to Drought Stress: A Review

Ngara, Rudo; Goche, Tatenda; Swanevelder, Dirk; Chivasa, Stephen

doi:10.3390/life11070704

Cited by 24 publications

(24 citation statements)

References 113 publications

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“…Transcriptional responses comprise multiple sets of drought-responsive genes that are spatiotemporally repressed or induced in an intricate manner 9 . Global gene expression regulation has already been investigated in sugarcane plants under drought conditions 10 – 18 .…”

Section: Discussionmentioning

confidence: 99%

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Leaf transcriptome profiling of contrasting sugarcane genotypes for drought tolerance under field conditions

Contiliani

Nebó

Ribeiro

et al. 2022

Sci Rep

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Drought is the most detrimental abiotic stress to sugarcane production. Nevertheless, transcriptomic analyses remain scarce for field-grown plants. Here we performed comparative transcriptional profiling of two contrasting sugarcane genotypes, ‘IACSP97-7065’ (drought-sensitive) and ‘IACSP94-2094’ (drought-tolerant) grown in a drought-prone environment. Physiological parameters and expression profiles were analyzed at 42 (May) and 117 (August) days after the last rainfall. The first sampling was done under mild drought (soil water potential of −60 kPa), while the second one was under severe drought (soil water potential of −75 kPa). Microarray analysis revealed a total of 622 differentially expressed genes in both sugarcane genotypes under mild and severe drought stress, uncovering about 250 exclusive transcripts to ‘IACSP94-2094’ involved in oxidoreductase activity, transcriptional regulation, metabolism of amino acids, and translation. Interestingly, the enhanced antioxidant system of ‘IACSP94-2094’ may protect photosystem II from oxidative damage, which partially ensures stable photochemical activity even after 117 days of water shortage. Moreover, the tolerant genotype shows a more extensive set of responsive transcription factors, promoting the fine-tuning of drought-related molecular pathways. These results help elucidate the intrinsic molecular mechanisms of a drought-tolerant sugarcane genotype to cope with ever-changing environments, including prolonged water deficit, and may be useful for plant breeding programs.

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Section: Discussionmentioning

confidence: 99%

“…Plant adaptation to drought stress is tightly controlled by a coordinated set of signaling networks, which switch on/off clusters of genes that directly affect the plant response 9 . Drought-associated gene expression changes were thoroughly investigated in leaves of several plant species, including sugarcane 10 – 17 .…”

Section: Introductionmentioning

confidence: 99%

Leaf transcriptome profiling of contrasting sugarcane genotypes for drought tolerance under field conditions

Contiliani

Nebó

Ribeiro

et al. 2022

Sci Rep

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show abstract

“…Proteomics studies are therefore crucial to understand plant response and identify key proteins that determine the outcomes of plant response to particular stresses. As such, proteomics studies have received increasing attention to deepen our understanding at molecular-level with regard to sorghum response to drought stress (Ngara et al 2021 ). The proteomic analysis of drought tolerant (El9) and sensitive sorghum genotypes exposed to drought stress identified proteins only expressed in drought-tolerant genotype, such as HSPs, GrpE protein homolog and Glycine-rich RNA-binding protein (GR-RBP) (Fadoul et al 2018 ).…”

Section: Proteome Response To Drought Stress In Sorghummentioning

confidence: 99%

“…Plant response to drought stress and drought tolerance is a result of complex biological processes involving physiological, biochemical, genomic, proteomic and metabolomics changes (Ngara et al 2021). An overview The two studies used different sorghum genotypes of drought stress and sorghum plant responses is provided in Fig.…”

Section: Physiological Mechanisms Of Drought Tolerance In Sorghummentioning

confidence: 99%

Sorghum in dryland: morphological, physiological, and molecular responses of sorghum under drought stress

Abreha

Enyew

Carlsson

et al. 2021

Planta

113

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Main conclusion Droughts negatively affect sorghum’s productivity and nutritional quality. Across its diversity centers, however, there exist resilient genotypes that function differently under drought stress at various levels, including molecular and physiological. Abstract Sorghum is an economically important and a staple food crop for over half a billion people in developing countries, mostly in arid and semi-arid regions where drought stress is a major limiting factor. Although sorghum is generally considered tolerant, drought stress still significantly hampers its productivity and nutritional quality across its major cultivation areas. Hence, understanding both the effects of the stress and plant response is indispensable for improving drought tolerance of the crop. This review aimed at enhancing our understanding and provide more insights on drought tolerance in sorghum as a contribution to the development of climate resilient sorghum cultivars. We summarized findings on the effects of drought on the growth and development of sorghum including osmotic potential that impedes germination process and embryonic structures, photosynthetic rates, and imbalance in source-sink relations that in turn affect seed filling often manifested in the form of substantial reduction in grain yield and quality. Mechanisms of sorghum response to drought-stress involving morphological, physiological, and molecular alterations are presented. We highlighted the current understanding about the genetic basis of drought tolerance in sorghum, which is important for maximizing utilization of its germplasm for development of improved cultivars. Furthermore, we discussed interactions of drought with other abiotic stresses and biotic factors, which may increase the vulnerability of the crop or enhance its tolerance to drought stress. Based on the research reviewed in this article, it appears possible to develop locally adapted cultivars of sorghum that are drought tolerant and nutrient rich using modern plant breeding techniques.

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“…1). These processes are important in conserving water and alleviating osmotic pressure damage imposed by drought (Ngara et al, 2021).…”

Section: Alterations In Transcriptome Under Drought Conditionmentioning

confidence: 99%

Alterations in Transcriptome, Proteome and Metabolome During Drought Stress Inplants

JOSHI¹,

BISHT²,

LOHANI³

2022

AJMBES

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Global climate changes may lead to increased temperature and decreased precipitation in manyparts of the world. Sustainable food production is the main challenge to our current agricultural system. Atthe global level, water stress is one of the most critical abiotic factorsthat affect crop productivity. Currently,a vast amount of research efforts are being done to generate molecular information leading to anunderstanding of abiotic stress tolerance in plants. Transcriptome analysis is a cost-effective and suitableapproach for the identification of candidate genes for adaptive traits and molecular markers that are linkedto phenotypic variation under drought. Proteome analysis is helpful in deciphering the cellular processesassociated with drought. Although gene expression analysis at the transcription level has enhanced ourunderstanding regarding the response of plants to drought stress, many questions remain unanswered atthe protein level. In view of the fact that the plant proteome is highly dynamic in nature, proteomics isbecoming essential for the study of many different aspects of plant functions. In plants, water scarcity affectsmorphological, physiological, and biochemical processes, including carbon assimilation and partitioning,respiration, nutrient uptake, translocation, and whole metabolism. Water deficit primarily affects the cellturgidity and promotes stomatal closure, controlling transpiration rates, and water loss. It will alsounavoidably restrict CO2 supply to photosynthesis, ultimately impairing leaf metabolism and plant growth.Alterations in transcriptome, proteome and metabolome help plant to recover from drought and survive.

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Sorghum’s Whole-Plant Transcriptome and Proteome Responses to Drought Stress: A Review

Cited by 24 publications

References 113 publications

Leaf transcriptome profiling of contrasting sugarcane genotypes for drought tolerance under field conditions

Leaf transcriptome profiling of contrasting sugarcane genotypes for drought tolerance under field conditions

Sorghum in dryland: morphological, physiological, and molecular responses of sorghum under drought stress

Alterations in Transcriptome, Proteome and Metabolome During Drought Stress Inplants

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