Sorption kinetics of binary protein solutions: General approach to multicomponent systems

High-performance liquid chromatography as a technique to measure the competitive adsorption of plasma proteins onto latices

“…These include depletion techniques (5-11, 32-34, 62), infrared spectroscopy (12-15, 22, 61), circular dichroism (8,16), total internal reflection fluorescence (21,24), ellipsometry (17,18), interfacial tension measurements (!9, 20, 28, 49, 55-57), electron microscopy (59), and the use of proteins labeled with fluorescent (19,37,38) or radioactive (25-36, 39-54, 63) compounds.…”

Adsorption of a Formulated Protein on a Drug Delivery Device Surface

“…5D. The mechanism is similar in spirit to those suggested by Beissinger and Leonard (3,48) and Soderquist and Walton (42). amide I band envelope has broadened, indicating a further departure from native structure.…”

“…where D is the inner diameter of the tubing, D is the diffusion This situation is described by the Levêque equation which coefficient of IL-2, and t tubing is the residence time for one is a solution of the convection-diffusion equation under passage through the manifold and linking tubing; note that diffusion-limited conditions for laminar flow adjacent to the the only significant mass transport mechanism to the tubing surface with adsorption occurring at the interface (3,50). wall at these low Reynolds number flows is diffusion.…”

Effects of plasma protein adsorption on protein conformation and activity

“…Total internal reflection fluorescence (TIRF) spectroscopy has recently been applied by several groups (22)(23)(24)(25)(26)(27) and complete reviews are now available (22,23). The method can easily follow the kinetics of adsorption using proteins labelled with extrinsic fluors, such as fluorescein or rhodamine.…”