2024
DOI: 10.1101/2024.03.05.583518
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Sortase A-based post-translational modifications on encapsulin nanocompartments

Seyed Hossein Helalat,
Yi Sun,
Rodrigo Tellez

Abstract: Protein-based encapsulin nanocompartments, known for their well-defined structures and versatile functionalities, present promising opportunities in the biotechnology and nanomedicine area. In this investigation, we effectively developed a sortase A-mediated protein ligation system inE. colito site-specifically attach target proteins to encapsulin, both internally and on its surfaces without any furtherin vitrosteps. We explored the potential applications of fusing sortase enzyme and a protease for post-transl… Show more

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