Sortase A-Based Post-translational Modifications on Encapsulin Nanocompartments
Seyed Hossein Helalat,
Rodrigo Coronel Téllez,
Ehsan Ansari Dezfouli
et al.
Abstract:Protein-based encapsulin nanocompartments, known for their well-defined structures and versatile functionalities, present promising opportunities in the fields of biotechnology and nanomedicine. In this investigation, we effectively developed a sortase A-mediated protein ligation system in Escherichia coli to site-specifically attach target proteins to encapsulin, both internally and on its surfaces without any further in vitro steps. We explored the potential applications of fusing sortase enzyme and a protea… Show more
Our study demonstrates that encapsulating tandem nitroreductase NfsB within encapsulins, along with pore mutations, enhances enzyme activity, with cryo-EM revealing its structural architecture and multiple pore states.
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