Source, alterations, characteristics and use of a new dog cell line (Cf2Th)

Nelson‐Rees, Walter A.; Owens, Robert B.; Arnstein, Paul; Kniazeff, Alexis J.

doi:10.1007/bf02797468

Cited by 42 publications

(23 citation statements)

References 18 publications

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“…Immortalized mammalian cell lines Cf2Th (canine thymus, ATCC CRL 1430) (35), HeLa (human carcinoma, ATCC CCL 2) (14), and NIH 3T3 (mouse fibroblast, ATCC CRL 1658) (2,12,19) were maintained in Dulbecco's modified Eagle medium with 5% bovine serum at 37°C with 5% CO 2 . Immortalized WF-2 walleye fry fibroblasts and primary W12 fibroblasts were maintained in HEPES-buffered minimum essential medium (pH 7.4) with Hanks salts and 10% fetal bovine serum at 20°C.…”

Section: Methodsmentioning

confidence: 99%

Walleye Dermal Sarcoma Virus Cyclin Interacts with Components of the Mediator Complex and the RNA Polymerase II Holoenzyme

Rovnak

Quackenbush

2002

J Virol

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Walleye dermal sarcoma virus (WDSV) encodes an accessory protein, OrfA, with sequence homology to cyclins (retrovirus cyclin). In cells transfected with an expression construct, OrfA was localized to the nucleus and was concentrated in interchromatin granule clusters (IGCs), sites where splicing factors are concentrated. Other proteins identified in IGCs include transcription factors, the large subunit of RNA polymerase II (Pol II), and cyclin-dependent kinase 8 (cdk8). cdk8 is the kinase partner of cyclin C and a component of the mediator complex, associated with the Pol II holoenzyme. cdk8 and cyclin C can regulate transcription via phosphorylation of cyclin H and the carboxy-terminal domain of Pol II. OrfA in transfected HeLa cells was found to colocalize and copurify with hyperphosphorylated forms of Pol II (Pol IIO) in IGCs, and OrfA was coimmunoprecipitated from lysates of transfected cells with an antibody against Pol IIO. Likewise, Pol IIO could be coprecipitated with an antibody against OrfA. A survey with antibodies against several different cdks resulted in coimmunoprecipitation of OrfA with anti-cdk8, and antiserum against OrfA was able to coprecipitate cdk8 from lysates of cells that express OrfA. Coprecipitation of OrfA with anti-cyclin C demonstrated that it was included in complexes with OrfA and cdk8. OrfA has sequence and structural similarities to cyclin C, and, functionally, OrfA appears to have the capacity to both enhance and inhibit the activity of promoters in a cell-specific manner, similar to functions of the mediator complex. These data suggest that WDSV OrfA functions through its interactions with these large, transcription complexes. Further investigations will clarify the role of the retrovirus cyclin in control of virus expression and transformation.Walleye dermal sarcoma virus (WDSV) is a member of the newly defined genus Epsilonretrovirus, of the family Retroviridae. WDSV is a complex retrovirus that is etiologically associated with dermal sarcomas in walleye fish (Stizostedion vitreum) (28,29,41,45,46). Rapid onset and high efficiency characterize the experimental transmission of this disease (7). Dermal sarcomas develop and regress seasonally, and the shift to regression is associated with qualitative and quantitative differences in viral gene expression: developing tumors contain low levels of two subgenomic transcripts, and regressing tumors contain approximately 1,000-fold-greater levels of fulllength viral RNA, a variety of spliced transcripts, and unintegrated viral DNA (6,7,37).WDSV contains three large open reading frames in addition to the gag, pol, and env genes, orfa and orfb, located in the 3Ј-proximal region of the genome, and orfc, located between the 5Ј long terminal repeat (LTR) and gag (18). The OrfA protein of WDSV and homologous proteins, encoded by walleye epidermal hyperplasia virus type 1 (WEHV-1) and WEHV-2, have limited homology to cyclins and are referred to as retrovirus cyclins (23). Viral oncogenes transduced by retroviruses demonstrate significant ho...

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Section: Methodsmentioning

confidence: 99%

Walleye Dermal Sarcoma Virus Cyclin Interacts with Components of the Mediator Complex and the RNA Polymerase II Holoenzyme

Rovnak

Quackenbush

2002

J Virol

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“…NIH 3T3 (TK-) mouse fibroblasts (16), PA12 amphotropic retrovirus packaging cells (5), and CF2TH canine fibroblasts (17) have been described. Cells were grown in Dulbecco modified Eagle medium (DME medium) with 4.5 g of glucose per liter and 10% fetal bovine serum.…”

Section: Methodsmentioning

confidence: 99%

Retroviral transfer of genes into canine hemopoietic progenitor cells in culture: a model for human gene therapy.

Kwok¹,

Schuening²,

Stead³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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“…Les cellules Cf2Th témoins [ 12] et celles des lignées cellulaires infectées d'une façon persistante par le V AIE (ADRI-Cf2Th/EIA V * ) [10] et le VIB (ADRI-Cf2Th/BMVLV * ) [13] furent cultivées en milieu Dulbecco MEM (DMEM) (GIBCO, Grand Island, N.Y., E.-U. ), enrichi de 10 % de sérum de foetus bovin (SFB).…”

Section: Materiel Et Methodesunclassified

“…Les résultats incluent des données préliminaires [10,11] illustrant certaines étapes du développement du modèle et la stratégie employée. La lignée cellulaire de thymus canin [12] est le modèle proposé parce qu'elle est réceptive aux deux virus ci-dessus [6,13].…”

Section: Introductionunclassified

Modèle in vitro de production d'antigènes, appliqué aux virus de l'anémie infectieuse des équidés et de l'immunodéficience bovine

Bouillant¹,

Nielsen²,

Lutze-Wallace³

1989

bavf

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of such models depends on criteria such as : 1) viral permissiveness of cells in a continuous line; 2) persistent infection; 3) continuous production of anti i gen in a serum-free medium. The second step involves the large-scale production of antigen in a bioreator. The first step was aohieved by initiating persistent infection of two canine cell lines by EIAV and BIV respectively. However, the method of BIV antigen production has not been completed due to the lack of adequate serological tests for detection of antigens. The second step involving both viroses is under study.Key words : Equine infectious anemia and bovine immunodeficiency..J.ike viroses -In vitro anti,gen production -Continuous cell .lines.

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Source, alterations, characteristics and use of a new dog cell line (Cf2Th)

Cited by 42 publications

References 18 publications

Walleye Dermal Sarcoma Virus Cyclin Interacts with Components of the Mediator Complex and the RNA Polymerase II Holoenzyme

Walleye Dermal Sarcoma Virus Cyclin Interacts with Components of the Mediator Complex and the RNA Polymerase II Holoenzyme

Retroviral transfer of genes into canine hemopoietic progenitor cells in culture: a model for human gene therapy.

Modèle in vitro de production d'antigènes, appliqué aux virus de l'anémie infectieuse des équidés et de l'immunodéficience bovine

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