SOX10 induced Nestin expression regulates cancer stem cell properties of TNBC cells

Feng, Wen; Liu, Si-hai; Zhu, Ruixia; Li, Baojian; Zhu, Zhu; Yang, Jiajun; Song, Chun-hui

doi:10.1016/j.bbrc.2017.02.014

Cited by 29 publications

(26 citation statements)

References 20 publications

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“…For migration to begin, neural crest cells must undergo a process called delamination that involves a full or partial EMT [ 26 ]. In fact, the SOX family, especially SOX10, identified in the present study, regulates cancer stem cell properties of TNBC cells [ 27 ] and participate in early determination and migration [ 28 – 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, the member of the SOX family, SRY-Box 9 (SOX9), was reported to play a critical role in the TNBC cell proliferation, migration, and invasion [ 44 ]. SRY-Box 10 (SOX10) induced nestin expression regulates cancer stem cell properties of TNBC cells [ 27 ]. Besides, SOX10 preferentially expressed in TNBC that it could be used as a promising diagnostic marker [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

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Identification of Key Genes and Pathways in Triple-Negative Breast Cancer by Integrated Bioinformatics Analysis

Dong

Pan

et al. 2018

BioMed Research International

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Purpose Triple-negative breast cancer refers to breast cancer that does not express estrogen receptor (ER), progesterone receptor (PR), or human epidermal growth factor receptor 2 (Her2). This study aimed to identify the key pathways and genes and find the potential initiation and progression mechanism of triple-negative breast cancer (TNBC). Methods We downloaded the gene expression profiles of GSE76275 from Gene Expression Omnibus (GEO) datasets. This microarray Super-Series sets are composed of gene expression data from 265 samples which included 67 non-TNBC and 198 TNBC. Next, all the differentially expressed genes (DEGs) with p<0.01 and fold change ≥1.5 or ≤-1.5 were identified. Result 56 upregulated and 151 downregulated genes were listed and the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analysis was performed. These significantly changed genes were mainly involved in the biological process termed prostate gland morphogenesis, inner ear morphogenesis, cell maturation, digestive tract morphogenesis, autonomic nervous system development, monovalent inorganic anion homeostasis, neural crest cell development, regulation of dendrite extension and glial cell proliferation, immune system process termed T cell differentiation, regulation of immune response, and macrophage activation. Genes are mainly involved in the KEGG pathway termed Oocyte meiosis. All DEGs underwent survival analysis using datasets from The Cancer Genome Atlas (TCGA) integrated by cBioPortal, of which amplification of SRY-related HMG-box 8 (SOX8), androgen receptor (AR), and Chromosome 9 Open Reading Frame 152 (C9orf152) were significantly negative while Nik Related Kinase (NRK) and RAS oncogene family 30 (RAB30) were positively correlated to the life expectancy (p<0.05). Conclusions In conclusion, these pathways and genes identified could help understanding the mechanism of development of TNBC. Besides, SOX8, AR, C9orf152, NRK and RAB30, and other key genes and pathways might be promising targets for the TNBC treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of Key Genes and Pathways in Triple-Negative Breast Cancer by Integrated Bioinformatics Analysis

Dong

Pan

et al. 2018

BioMed Research International

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“…In lung adenocarcinoma, SOX-2 promotes NES mRNA transcription (42), while in human melanoma cells SOX-9 and SOX-10 are required for the induction of nestin expression (43). Recently, Feng et al demonstrated that in TNBCs nestin expression was induced by SOX-10, resulting in the regulation of stem-like features of TNBC cells (4). The association between nestin expression in tumour cells and vascular density has been previously observed in hepatocellular carcinoma (44), ovarian carcinoma (45), glioblastomas (46) and ependymomas (47).…”

Section: Discussionmentioning

confidence: 99%

“…Triple-negative breast cancer (TNBC) comprises approximately 15% of all BC cases. The majority of TNBCs fall into the basal-like subtype, which is the most aggressive type of BC and is characterised by rapid tumour growth and a poor outcome (3,4). The aggressive characteristics of TNBC are presumably associated with an abundance of cancer stem cells (CSCs).…”

Section: Introductionmentioning

confidence: 99%

Role of nestin expression in angiogenesis and breast cancer progression

et al. 2017

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Nestin is an intermediate filament protein and a stem cell marker expressed in several tumours. There is growing evidence of an association between the expression level of nestin and the pathogenesis of triple-negative breast cancer (TNBC). Nestin is also expressed in newly forming tumour vessels and is a valuable marker of ongoing angiogenesis. In this study, we aimed to evaluate the prognostic value of nestin expression in breast tumour cells and to determine whether this expression influences angiogenesis. Immunohistochemical (IHC) analyses were carried out on 124 cases of invasive ductal carcinoma (IDC) of the breast with a panel of murine monoclonal antibodies against nestin, CD31, CD34, SOX-18 and Ki‑67. We evaluated nestin expression in tumour and endothelial cells, Ki‑67 in tumour cells, and CD31, CD34 and SOX-18 in endothelial cells. Our results demonstrated that nestin expression in tumour cells correlated with the area and number of vessels expressing nestin, CD31, CD34 and SOX-18. We also found a positive correlation between nestin-expressing vessels and SOX-18-expressing vessels. Our results are consistent with those of previous studies, in which nestin expression in endothelial cells was shown to be strongly associated with triple-negative subtype, poorly differentiated G3 tumours, a higher proliferation index and a shorter overall survival. Nestin expression was also examined in human breast cancer cell lines (MCF-7, SK-BR-3, MDA‑MB‑231 and BO2 cells) representing a different level of tumour aggressiveness and reflecting histological grade. A higher nestin protein level was observed in more aggressive MDA‑MB‑231 and BO2 cells than in MCF-7 and SK-BR-3 cells.

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“…The expression of nestin has been reported to be regulated by Sox10 (12,13). Thus, we examined the expression of Sox10 in parotid acinar cells by real time RT-PCR.…”

Section: Change In the Expression Level Of Rno-mir-3473 In Parotid Acmentioning

confidence: 97%

Change in Expression Patterns of Micro RNAs in the Primary Culture of Parotid Acinar Cells

et al. 2019

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Micro RNAs(miRNAs)are considered to regulate the development and dysfunction of salivary glands. Previous studies on the expression patterns of genes in the primary culture of parotid acinar cells revealed that the isolation process of acinar cells from the glands caused tissue injuries and activated the Src-p38 MAP kinase signaling pathway. While acinar markers were decreased, expression of stem/progenitor markers commenced in the culture. In this study, changes in expression patterns of miRNAs of parotid acinar cells were examined by microarray analysis in order to clarify the mechanism for changes in characteristics of the cells. Analysis on expression values after normalized with robust multi-array averaging method identified that 18 miRNAs were up-regulated and 52 miRNAs were down-regulated after 1-day culture compared with just after isolation. Pathway analysis on the expression-altered miRNAs suggested that they were involved in the regulation of multipotent stem cells. Among the down-regulated miRNAs that were highly expressed, miR-3473, whose fold change was the largest, significantly decreased during the culture for 2 days. Inversely, Sox10, a candidate of target genes for miR-3473, increased during the culture, which was confirmed by real time RT-PCR and immunoblot analyses. Inhibition of Src kinase activity suppressed the changes in expression levels of miR-3473 and Sox10. Since Sox10 is involved in maintenance of stemness and promotes nestin expression, there is a possibility that the down-regulation of miRNAs may regulate acquisition of stemness in parotid acinar cells.

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SOX10 induced Nestin expression regulates cancer stem cell properties of TNBC cells

Cited by 29 publications

References 20 publications

Identification of Key Genes and Pathways in Triple-Negative Breast Cancer by Integrated Bioinformatics Analysis

Identification of Key Genes and Pathways in Triple-Negative Breast Cancer by Integrated Bioinformatics Analysis

Role of nestin expression in angiogenesis and breast cancer progression

Change in Expression Patterns of Micro RNAs in the Primary Culture of Parotid Acinar Cells

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