Soy β-conglycinin improves glucose uptake in skeletal muscle and ameliorates hepatic insulin resistance in Goto-Kakizaki rats

Tachibana, Nobuhiko; Yamashita, Yoko; Nagata, Morio; Wanezaki, Satoshi; Ashida, Hitoshi; Horio, Fumihiko; Kohno, M.

doi:10.1016/j.nutres.2013.12.001

Cited by 33 publications

(34 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We previously reported that consumption of βCG improves postprandial hyperglycemia by promoting GLUT4 translocation to the plasma membrane in skeletal muscle of a rat model of diabetes (Tachibana, et al, 2014). This suggests that βCG may act directly and/or indirectly in GLUT4 translocation.…”

Section: Discussionmentioning

confidence: 92%

“…Many reports have demonstrated that soy protein and its major component, βCG, have the potential to prevent and treat hyperglycemia and diabetes mellitus (Cederroth, et al, 2008;Lu, et al, 2008;Yang, et al, 2008;Tachibana, et al, 2014). We previously reported that consumption of βCG improves postprandial hyperglycemia by promoting GLUT4 translocation to the plasma membrane in skeletal muscle of a rat model of diabetes (Tachibana, et al, 2014).…”

Section: Discussionmentioning

confidence: 94%

“…It has been reported that consumption of βCG has an anti-obesity effect through the reduction of plasma triglycerides (Tachibana, et al, 2010;Kohno, et al, 2006). In addition, our group recently reported that consumption of βCG promotes GLUT4 translocation through the AMPK signaling pathway in a rat model of diabetes (Tachibana, et al, 2014). Adiponectin is known as an AMPK activator (Kadowaki and Yamauchi, 2005), and βCG increases adiponectin levels in serum and its expression level in visceral fat (Tachibana, et al, 2014).…”

Section: Introductionmentioning

confidence: 96%

“…In addition, our group recently reported that consumption of βCG promotes GLUT4 translocation through the AMPK signaling pathway in a rat model of diabetes (Tachibana, et al, 2014). Adiponectin is known as an AMPK activator (Kadowaki and Yamauchi, 2005), and βCG increases adiponectin levels in serum and its expression level in visceral fat (Tachibana, et al, 2014). However, AMPK activation is not observed in the livers of mice fed with βCG.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

β-Conglycinin Peptides Improve Glucose Uptake through the AMPK Signaling Pathway in L6 Myotubes

Yamashita

Ueda-Wakagi

Sakamoto

et al. 2015

FSTR

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

β-Conglycinin Peptides Improve Glucose Uptake through the AMPK Signaling Pathway in L6 Myotubes

Yamashita

Ueda-Wakagi

Sakamoto

et al. 2015

FSTR

Self Cite

View full text Add to dashboard Cite

show abstract

“…Soy b-conglycinin, a constituent of soy proteins, has been reported to increase glucose uptake in muscles by activating the translocation of GLUT4 and AMP-activated protein kinase expression in GK rats (22). Whey protein hydrolysate has also been reported to increase the translocation of GLUT4 in muscles, which can induce glycogen accumulation in muscles (23).…”

Section: Resultsmentioning

confidence: 99%

Egg White Hydrolysate Improves Glucose Tolerance in Type-2 Diabetic NSY Mice

Ochiai

Azuma

2017

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

View full text Add to dashboard Cite

SummaryWe have previously reported that chicken egg white (EW) and low-allergenic EW hydrolysate (EWH) suppressed ectopic fat accumulation and improved serum glucose and insulin levels. In this study, the dietary effects of EW and EWH on glucose tolerance were investigated in different ways to clarify the effect of EW and EWH on intestinal glucose absorption. Type 2 diabetic Nagoya-Shibata-Yasuda mice were divided into four groups: a low-fat and low-sucrose casein-based diet group (NL); high-fat and high-sucrose (HFS) casein-based diet group (NH); HFS EW-based diet group (NE); and HFS EWH-based diet group (NEH). Mice were fed their respective diets for 8 wk. At the end of the 6th and 7th week, an oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were respectively conducted in experiment A. At the end of the 7th week, an intraperitoneal glucose tolerance test (ipGTT) was conducted in experiment B. In experiment A, the plasma glucose level was suppressed in the NE group during both OGTT and ITT, and suppressed in the NEH group during OGTT, but not during ITT. In experiment B, the plasma glucose level was similarly suppressed in the NEH group during ipGTT, but the suppressive effect was weakened compared to OGTT. Plasma insulin level was lower in the NE and NEH groups in both experiments. Fecal triacylglycerol excretion was increased in the NE and NEH groups in experiment A and liver triacylglycerol content was suppressed in the NE group in experiment B. These findings suggested that in addition to improving fat metabolism, EWH improves glucose tolerance via mechanisms related and unrelated to small intestinal function. Key Words egg white hydrolysate, glucose tolerance, oral administration, intraperitoneal injection, Nagoya-Shibata-Yasuda mice Chicken egg white (EW) makes up about 60% of the whole egg weight and contains proteins high in amino acid balance. EW has been reported to suppress food intake, dietary fat absorption, and fat accumulation in rats in our previous studies (1-3); therefore, EW can be considered for the prevention and treatment of obesity and type 2 diabetes mellitus (T2DM) in humans. However, egg-specific proteins in EW are well known to be major allergens in humans. Continuous consumption of EW or whole egg can induce allergic symptoms in humans; therefore, it is necessary to produce low-allergenic EW products. Recently, low-allergenic bioactive peptides derived from EW have been developed and purified for the prevention and improvement of several metabolic disorders such as hypertension and oxidative stress (4-6). However, few studies have reported the effects of low-allergenic EW products on glucose metabolism.We previously developed EW enzymatic hydrolysate (EWH), which contains less allergenic proteins and has similar physicochemical properties to EW (3). We have also reported that EWH improves fasting serum glucose and insulin levels in diabetic Goto-Kakizaki (GK) rats (1) and Wistar rats (2). These findings suggest that in addition to improving fat metabolism, EWH can...

show abstract

Evaluation of the contents and ratios of five fractionated proteins to elucidate the protein composition in soybean seeds

Sugiyama

Sakai

Ichinose

et al. 2023

J Americ Oil Chem Soc

View full text Add to dashboard Cite

Selecting suitable soybean cultivars is important for food processing and exploring their endowment with desirable physiological functions. We applied the fractionation method previously established to compare soy protein composition among cultivars to promote such a selection. More than 95% of the proteins in soybean cotyledons were extracted from 13 soybean cultivars using a high‐concentration salt solution. The extracted proteins were fractionated into five fractions, namely oil body‐associated protein (OBAP), polar lipid‐associated protein (PLAP), globulins (11S and 7S), and whey by centrifugation after tuning the solubility behavior of the proteins with various solutions. Protein species in each fraction were analyzed by SDS‐PAGE. Protein content in the total extract and five fractions was quantified to characterize the protein composition of soybean cultivars. The correlation between the protein content of each fraction and the total protein in cotyledon was investigated. A strong positive correlation was found only for the 11S fraction (r = 0.82), followed by a positive correlation in the 7S fraction (r = 0.65). Thus, we surmised that the increased protein content in soybean was due to increased globulin content. Furthermore, the calculation of the average ratio of protein content in each fraction indicated the globulin fraction (7S and 11S) to be 52%, the lipophilic protein fraction (OBAP and PLAP) to be 33%, and the whey fraction to be 13%. The preparation method employed in this study is a promising tool for efficiently comparing the protein composition of soybean cultivars to evaluate the potential use of cultivars for food production.

show abstract

Soy β-conglycinin improves glucose uptake in skeletal muscle and ameliorates hepatic insulin resistance in Goto-Kakizaki rats

Cited by 33 publications

References 33 publications

β-Conglycinin Peptides Improve Glucose Uptake through the AMPK Signaling Pathway in L6 Myotubes

β-Conglycinin Peptides Improve Glucose Uptake through the AMPK Signaling Pathway in L6 Myotubes

Egg White Hydrolysate Improves Glucose Tolerance in Type-2 Diabetic NSY Mice

Evaluation of the contents and ratios of five fractionated proteins to elucidate the protein composition in soybean seeds

Contact Info

Product

Resources

About