Soybean Extract Suppresses B Cell Activation Through TLR3/TLR4 in High Fat-High Fructose Diet Mice

Atho'illah, Mochammad Fitri; Safitri, Yunita Diyah; Nur’aini, Farida Dewi; Widyarti, Sri; Tsuboi, Hideo; Rifa’i, Muhaimin

doi:10.25002/tji.2018.866

Cited by 5 publications

(7 citation statements)

References 46 publications

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“…Atho’illah et al. demonstrated that ESE decline TLR3 and TLR4 in B cells of HFFD mice [ 24 ]. Another phytoalexin contains in grape, resveratrol, inhibits TBK1, and RIP1 in TRIF complex [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

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Elicited soybean extract attenuates proinflammatory cytokines expression by modulating TLR3/TLR4 activation in high−fat, high−fructose diet mice

Atho'illah

Safitri²,

Nur’aini

et al. 2021

Journal of Ayurveda and Integrative Medicine

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Background The high−fat, high−fructose diet (HFFD) provokes overnutrition and inflammation directly, mainly through Toll−like receptors (TLRs). Soybean ( Glycine max L.) contains isoflavone that can be transformed into glyceollin by microbial and physical stimuli. Glyceollin possesses many beneficial effects on health. Objective This study evaluates the beneficial effect of soybean extract elicited by Saccharomyces cerevisiae and light (ESE) on dendritic cells (DCs) profile and naïve T cells in HFFD mice. Materials and methods Female Balb/C mice were fed with HFFD for 24 weeks then orally administered with simvastatin 2.8 mg/kg BW or ESE 78, 104, and 130 mg/kg BW at the last four weeks. The expression of splenic CD11c + TLR3 + , CD11c + TLR4 + , NFκB + , CD11c + IL-17 + , CD11c + TNF−α + , CD4 + CD62L + , and CD8 + CD62L + subsets was measured by flow cytometry. The molecular docking has been measured using Pyrx 0.8, displayed in PyMol and Biovia Discovery Studio. Result HFFD significantly increased CD11c + TLR3 + , CD11c + TLR4 + , NFκB + , CD11c + IL-17 + , CD11c + TNF−α + expression and decreased CD4 + CD62L + and CD8 + CD62L + ( p < 0.05) compared to normal diet (ND) groups. ESE reduced CD11c + TLR3 + , CD11c + TLR4 + , thereby decreasing NFκB + , as well as decreased the CD11c + IL−17 + , CD11c + TNF−α + , and restores CD4 + CD62L + and CD8 + CD62L + subsets in HFFD mice. Glyceollin II exhibited the best binding affinity with an average energy of −7.3 kcal/mol to TLR3 and −7.9 kcal/mol to TLR4. Conclusion The bioactive compound in ESE act synergistically to modulate TLR3/TLR4 activation, reduced NFκB, IL−17, and TNF−α, and restores naïve T cells expression in HFFD mice. ESE was a favorable candidate to mitigate chronic inflammation.

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Section: Discussionmentioning

confidence: 99%

“…The spleen samples were taken, washed twice with sterile PBS, and smashed into a single-cell suspension. A single-cell suspension of the spleen has been prepared as per routine laboratory procedures [ 24 ]. Subsequent splenic lymphocytes were centrifuged at 2500 rpm for 5 min at 10 °C.…”

Section: Methodsmentioning

confidence: 99%

Elicited soybean extract attenuates proinflammatory cytokines expression by modulating TLR3/TLR4 activation in high−fat, high−fructose diet mice

Atho'illah

Safitri²,

Nur’aini

et al. 2021

Journal of Ayurveda and Integrative Medicine

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“…Single-cell suspensions then added with PBS until reached 10 mL and centrifuged at 2500 rpm for 5 min at 10°C. The supernatant then discarded and the pellet was homogenized with 1 mL PBS [20]. Homogenates then divided into several 1.5 mL tubes according to the staining used then centrifuged at 2500 rpm for 5 min at 10°C.…”

Section: Methodsmentioning

confidence: 99%

Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization

et al. 2019

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Background and Aim: Anisakis is a global challenge for a fish product which may lead to a decrease in economic value and consumers' preference. Skipjack (Katsuwonus pelamis) in Kupang, Nusa Tenggara Timur, Indonesia, have important economic value for local fisheries. Anisakis typica is one of the Anisakis species which potent to induce an allergic reaction. However, the study about A. typica involved in the dendritic cells (DCs), T helper 1 (Th1), T helper 2 (Th2), and regulatory T cells (Tregs) is still limited. This study aimed to analyze the dynamic changed of the immune system including DCs, CD4+ T cells, and Tregs after 1 week of A. typica sensitization. Materials and Methods: Twenty-four male Balb/C mice were randomly divided into four groups (n=6), mice treated with crude A. typica extract (CAE) 50, 75, and 100 mg/kg BW, respectively. CAE was given orally per day for a week. At the end of the experiment, the animals were sacrificed and the spleen was collected. DCs were labeled as CD11c+ interleukin-6+ (IL-6+); CD4+ T cells were distinguished as Th1 (CD4+ interferon-γ+ [IFN-γ+]) and Th2 (CD4+ IL-4+ and CD4+ IL-5+); Tregs were labeled as CD4+CD25+CD62L+. The expression of each cell was determined by flow cytometry. Results: Our result described that CAE elicits CD11c+ IL-6+, CD4+ IFN-γ+, CD4+ IL-4+, and CD4+ IL-5+ and reduces CD4+CD25+CD62L+ significantly (p<0.05) in dose-dependent manner in mice after A. typica infection. Conclusion: The Th1/Th2 ratio after A. typica crude extract treatment exhibits a mixed pattern rather than the classical model allergy to food antigens. Our study is expected as a basic understanding of the changes in immune response after A. typica infection.

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“…The suspension was then put into a 15 mL polypropylene centrifuge tube. (20) The lymphocyte density was calculated under the light microscope using a hemocytometer and trypan blue staining. (21) The number of lymphocytes was counted in 5 large squares of the hemocytometer.…”

Section: Measurement Of Lymphocyte Densitymentioning

confidence: 99%

Single bulb garlic oil improves interleukin-6 via decreased reactive oxygen species (ROS) in high-fat diet male mice

2019

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Background High-fat diet (HFD) is highly responsible for the development of excessive reactive oxygen species (ROS). ROS and low-density lipoprotein (LDL) then trigger macrophage activation to secrete interleukin-6 (IL-6). Single bulb garlic or in Indonesian called bawang lanang is traditional medicine that possesses strong antioxidant properties. This study aimed to evaluate the effect of single bulb garlic oil (SBGO) on ROS, IL-6, and lymphocyte density in HFD-fed male mice. Methods This was an experimental study on 24 male mice randomly subdivided into 6 groups: one group was fed a normal diet, whereas the remaining five groups were fed HFD for 45 days and were then treated with single bulb garlic oil 0 mg/kg, simvastatin 26 mg/kg BW, single bulb garlic oil 12.5 mg/kg BW, 25 mg/kg BW, and 50 mg/kg BW respectively for the next 35 days. At the end of treatment, the mice were dissected for collection of (i) serum in order to measure ROS and IL-6 levels using ELISA, (ii) aortas to measure IL-6 expression using immunohistochemistry-fluorescence (IHC-F) and to obtain lymphocytes from bone marrow and spleen which were then counted using a light microscope. Results Our results indicated that SBGO decreased the ROS level, IL-6 level, IL-6 expression, and lymphocyte density in HFD mice. SBGO 12.5 mg/kg BW is the optimum dose in our study to reduce inflammation in HFD male mice. Conclusion SBGO is useful to reduce inflammation and improve antioxidant imbalance, thus might be a potential antiatherogenic agent.

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Soybean Extract Suppresses B Cell Activation Through TLR3/TLR4 in High Fat-High Fructose Diet Mice

Cited by 5 publications

References 46 publications

Elicited soybean extract attenuates proinflammatory cytokines expression by modulating TLR3/TLR4 activation in high−fat, high−fructose diet mice

Elicited soybean extract attenuates proinflammatory cytokines expression by modulating TLR3/TLR4 activation in high−fat, high−fructose diet mice

Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization

Single bulb garlic oil improves interleukin-6 via decreased reactive oxygen species (ROS) in high-fat diet male mice

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