Soybean Stem Fly, Melanagromyza sojae (Diptera: Agromyzidae), in the New World: detection of high genetic diversity from soybean fields in Brazil

Arnemann, Jonas André; Tay, Wee Tek; Walsh, Tom; Brier, Hugh; Gordon, Karl; Hickmann, Frederico; Ugalde, Gustavo A.; Guedes, J. V. C.

doi:10.4238/gmr.15028610

Cited by 22 publications

(35 citation statements)

References 27 publications

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“…From the 23 M. sojae specimens collected from Paraguay, we identified a total of 15 haplotypes, eight of which were novel haplotypes, 6 were identical to previously reported Brazil haplotypes (i.e., 'Msoj-COI-01', 'Msoj-COI-02', 'Msoj-COI-03', 'Msoj-COI-07', 'Msoj-COI-08', 'Msoj-COI-09'; Arnemann et al, 2016b), and one with Australia (haplotype 'Msoj-COI-02' (Arnemann et al, 2016b), individuals 17 and 18, from Pirapó, Table 1). …”

Section: Discussionmentioning

confidence: 71%

“…Post-sequencing trimming of the sequenced amplicons resulted in 740-bp partial mtDNA COI contigs in all samples. The low estimates of evolutionary divergence between M. sojae sequences were as expected at the intra-species level (e.g., Scheffer, 2000;Arnemann et al, 2016b) and ranged from 0 to 0.012% (± 0.001-0.004 SE). The evolutionary divergence between SSF haplotypes from Paraguay and Brazil was 0.005%, and between Paraguay and Australia was 0.003%.…”

Section: Resultsmentioning

confidence: 92%

“…PCR conditions for the mtDNA COI primer pairs were as previously reported (Arnemann et al, 2016b). The PCR amplicons were sent to Helixxa, Paulínia-SP-Brazil, for sequencing.…”

Section: Pcr Amplification and Sequencing Of The Partial Mtdna Coi Genementioning

confidence: 99%

“…We used SSF-specific partial mtDNA COI gene PCR primers (SSF-COI-F01: 5'-GAC AATGATTATTTTCGACAAAT-3'; SSF-COI-R01: 5'-GTAAAATAAGCTCGTGTATCTACA TC-3') developed by Arnemann et al (2016b) for molecular characterization of the suspected SSF individuals. PCR conditions for the mtDNA COI primer pairs were as previously reported (Arnemann et al, 2016b).…”

Section: Pcr Amplification and Sequencing Of The Partial Mtdna Coi Genementioning

confidence: 99%

“…We checked for potential presence of premature stop codons by translating the partial mtCOI sequences into protein sequences by selecting the invertebrate genetic code 5 for amino acid translation. Nucleotide distances (uncorrected P-distance) between specimens from Paraguay and all previously reported mtDNA COI haplotypes from Brazil and Australia (Arnemann et al, 2016b) were calculated in MEGA (Tamura et al, 2013), and maximum likelihood phylogeny was inferred using the web-based program PhyML (Guindon et al, 2010) using the automatic model selection option followed by 1000 bootstrap replications to estimate node confidence. Selection of out-group species Ophiomyia quinta (EF104665) and Ophiomyia nasuta (EF104661) for the phylogenetic analysis was based on the study of Scheffer et al (2007).…”

Section: Sequence Analysis and Molecular Characterization Of The Mtdnmentioning

confidence: 99%

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Research Article First record of soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae) in Paraguay confirmed by molecular evidence.

Guedes¹,

Arnemann²,

Curioletti³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. We provided the first scientific record of Melanagromyza sojae (Zehntner, 1900), through molecular characterization of partial mtDNA COI gene, that confirms the occurrence of this pest in Paraguay. Previously reported in Brazil, an outbreak of larvae of M. sojae known as the soybean stem fly (SSF) that belongs to the family Agromyzidae, was also noted in soybean fields from the Canindeyú, Alto Paraná and Itapúa Departments in Paraguay. This pest is highly polyphagous, attacking various host plant species from the family Fabaceae, such as soybean and other beans. The implications of SSF detection in 2 J.V.C. Guedes et al. Genetics and Molecular Research 16 (3): gmr16039707 Paraguay are discussed in relation to the current soybean cultivation practices from this agriculturally important South American region, including Brazil.

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Section: Discussionmentioning

confidence: 71%

Section: Resultsmentioning

confidence: 92%

“…PCR conditions for the mtDNA COI primer pairs were as previously reported (Arnemann et al, 2016b). The PCR amplicons were sent to Helixxa, Paulínia-SP-Brazil, for sequencing.…”

Section: Pcr Amplification and Sequencing Of The Partial Mtdna Coi Genementioning

confidence: 99%

Section: Pcr Amplification and Sequencing Of The Partial Mtdna Coi Genementioning

confidence: 99%

Section: Sequence Analysis and Molecular Characterization Of The Mtdnmentioning

confidence: 99%

See 3 more Smart Citations

Research Article First record of soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae) in Paraguay confirmed by molecular evidence.

Guedes¹,

Arnemann²,

Curioletti³

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

Highly diverse and rapidly spreading: Melanagromyza sojae threatens the soybean belt of South America

et al. 2021

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The soybean stem fly, Melanagromyza sojae, an Asian native insect, has successfully established in Brazil, Argentina, Paraguay and Bolivia. These countries are among the lead global soybean producing nations, being collectively known as the soybean belt of South America. Infestation levels of M. sojae grow by the year, facilitated by the lack of efficient management strategies. Previous studies have revealed a high number of maternal lineages in M. sojae populations from Southern Brazil and Paraguay, but a comprehensive survey on genetic diversity combining samples from all countries within the South American soybean belt remains absent. We used the mitochondrial DNA cytochrome oxidase I partial gene (mtCOI) to characterize specimens of M. sojae collected in fourteen Brazilian sites and one Argentine site, and then combined our mtCOI data with previously published data from Australia, Bolivia, Paraguay, and other Brazilian sites, to investigate genetic diversity in this invasive agricultural pest species. Based on the molecular characterisation of the mtCOI gene, haplotypes Msoj-COI-01 and Msoj-COI-02 have the highest frequencies in the continent. The

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First record of the soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae) in chickpea crops

Vera

Perini

Murúa

et al. 2021

Int J Trop Insect Sci

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Soybean Stem Fly, Melanagromyza sojae (Diptera: Agromyzidae), in the New World: detection of high genetic diversity from soybean fields in Brazil

Cited by 22 publications

References 27 publications

Research Article First record of soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae) in Paraguay confirmed by molecular evidence.

Research Article First record of soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae) in Paraguay confirmed by molecular evidence.

Highly diverse and rapidly spreading: Melanagromyza sojae threatens the soybean belt of South America

First record of the soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae) in chickpea crops

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