Sp1 in Astrocyte Is Important for Neurite Outgrowth and Synaptogenesis

Hung, Cheng‐Yuan; Hsu, Todd; Chuang, Jian Ying; Su, Tsung Ping; Chang, Wen Chang; Hung, Jan Jong

doi:10.1007/s12035-019-01694-7

Cited by 24 publications

(15 citation statements)

References 71 publications

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“…Specifically, FGF2, IL5 and SP1, through twelve target proteins, promote cell viability and migration and, on the other side, inhibit apoptosis and organismal death confirming the protective role of hill extract. In addition, a regulatory role of SP1 for neuronal development and synaptogenesis has been suggested in mice astrocytes [ 81 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Neuroprotective Activity of Extra Virgin Olive Oil Extracts Obtained from Quercetano Cultivar Trees Grown in Different Areas of the Tuscany Region (Italy)

et al. 2021

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Neurodegenerative diseases are driven by several mechanisms such as inflammation, abnormal protein aggregation, excitotoxicity, mitochondrial dysfunction and oxidative stress. So far, no therapeutic strategies are available for neurodegenerative diseases and in recent years the research is focusing on bioactive molecules present in food. In particular, extra-virgin olive oil (EVOO) phenols have been associated to neuroprotection. In this study, we investigated the potential antioxidant and neuroprotective activity of two different EVOO extracts obtained from Quercetano cultivar trees grown in two different areas (plain and hill) of the Tuscany region (Italy). The different geographical origin of the orchards influenced phenol composition. Plain extract presented a higher content of phenyl ethyl alcohols, cinnammic acids, oleacein, oleocanthal and flavones; meanwhile, hill extract was richer in lignans. Hill extract was more effective in protecting differentiated SH-SY5Y cells from peroxide stress thanks to a marked upregulation of the antioxidant enzymes heme oxygenase 1, NADPH quinone oxidoreductase 1, thioredoxin Reductase 1 and glutathione reductase. Proteomic analysis revealed that hill extract plays a role in the regulation of proteins involved in neuronal plasticity and activation of neurotrophic factors such as BDNF. In conclusion, these data demonstrate that EVOOs can have important neuroprotective activities, but these effects are strictly related to their specific phenol composition.

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Section: Discussionmentioning

confidence: 99%

Antioxidant and Neuroprotective Activity of Extra Virgin Olive Oil Extracts Obtained from Quercetano Cultivar Trees Grown in Different Areas of the Tuscany Region (Italy)

et al. 2021

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“…During gliogenesis, Sp1 was proven to binding to the promoter of GFAP, the expression of which was enhanced ( Yeo et al, 2013 ; Johnson et al, 2016 ). The loss of Sp1 in astrocytes is linked to learning and memory impairment in mice by GFAP decrease ( Hung et al, 2020 ). In our study, propofol increase the proportion of GFAP + cells after induced differentiation.…”

Section: Discussionmentioning

confidence: 99%

Propofol Exposure Disturbs the Differentiation of Rodent Neural Stem Cells via an miR-124-3p/Sp1/Cdkn1b Axis

Cao

Zeng

et al. 2020

Front. Cell Dev. Biol.

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Accumulating studies have indicated that propofol may lead to neurotoxicity and its effect on neural stem cells (NSCs) may play pivotal role in propofol-related neurotoxicity. Previously, we found that propofol could promote NSCs proliferation and could regulate several microRNA expressions. However, the underlying mechanism between microRNAs and NSCs development after propofol exposure is still unclear. Our data first observed that rat primary neural stem cells exposed to propofol exhibited a cell cycle arrest status and an inclination to differentiate into GFAP + or S100β + cells. This phenomenon was accompanying with a lower miR-124-3p expression and could be reversed via overexpression miR-124-3p in NSCs. Using bioinformatic predictions and luciferase assay we confirmed that Sp1 (Specificity Protein 1) is the target gene of miR-124-3p, indicating that miR-124-3p may regulate NSCs development through Sp1. Further, knockdown of Sp1 rescue the effect of propofol on NSCs differentiation. Finally, we demonstrated that Sp1 could bind cdkn1b promoter region through chromatin immunoprecipitation assay, indicating that Sp1 affect NSC's cell cycle through cdkn1b directly. Overall, our study highlights the miR-124-3p/Sp1/cdkn1b axis to be important in propofol interfering the differentiation of NSCs.

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“…They showed that Sp1 can be of great importance for astrocyte function. They found that Sp1 in astrocytes regulates the expression of several genes that are involved in the neuronal development process such as neurite outgrowth and synaptogenesis ( Augusto-Oliveira et al, 2020 ; Hung et al, 2020 ).…”

Section: The Role Of Astrocytes In Synaptogenesis During Brain Damagementioning

confidence: 99%

The Role of Astrocytes in the Neurorepair Process

Chiareli

Carvalho

Marques

et al. 2021

Front. Cell Dev. Biol.

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Astrocytes are highly specialized glial cells responsible for trophic and metabolic support of neurons. They are associated to ionic homeostasis, the regulation of cerebral blood flow and metabolism, the modulation of synaptic activity by capturing and recycle of neurotransmitters and maintenance of the blood-brain barrier. During injuries and infections, astrocytes act in cerebral defense through heterogeneous and progressive changes in their gene expression, morphology, proliferative capacity, and function, which is known as reactive astrocytes. Thus, reactive astrocytes release several signaling molecules that modulates and contributes to the defense against injuries and infection in the central nervous system. Therefore, deciphering the complex signaling pathways of reactive astrocytes after brain damage can contribute to the neuroinflammation control and reveal new molecular targets to stimulate neurorepair process. In this review, we present the current knowledge about the role of astrocytes in brain damage and repair, highlighting the cellular and molecular bases involved in synaptogenesis and neurogenesis. In addition, we present new approaches to modulate the astrocytic activity and potentiates the neurorepair process after brain damage.

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Sp1 in Astrocyte Is Important for Neurite Outgrowth and Synaptogenesis

Cited by 24 publications

References 71 publications

Antioxidant and Neuroprotective Activity of Extra Virgin Olive Oil Extracts Obtained from Quercetano Cultivar Trees Grown in Different Areas of the Tuscany Region (Italy)

Antioxidant and Neuroprotective Activity of Extra Virgin Olive Oil Extracts Obtained from Quercetano Cultivar Trees Grown in Different Areas of the Tuscany Region (Italy)

Propofol Exposure Disturbs the Differentiation of Rodent Neural Stem Cells via an miR-124-3p/Sp1/Cdkn1b Axis

The Role of Astrocytes in the Neurorepair Process

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