Engineering activities along the Sichuan–Tibet Railway (STR) could cause land degradation and threaten the surrounding ecological security. It is crucial to evaluate the integrated land ecology during and after the construction of this project. This study assesses the land ecology along the STR corridor from 2000 to 2022 using a transfer matrix, the analytic hierarchy process (AHP), and the PSR-TOPSIS model. The main results are as follows: (1) The novel comprehensive ecological assessment process including nine indicators is feasible. (2) The high-quality land ecological, surface vegetation, and environmental regions were concentrated in Ya’an and Nyingchi, whereas the low-quality regions were situated in Qamdo and Garze Tibetan Autonomous Prefecture. (3) There was an overall decline in the integrated land ecological quality along the STR from 2000 to 2022. While it steadily improved in the Ya’an and Nyingchi regions from 2010 to 2022, it continued to decline around the Qamdo region. (4) The most degraded land-use type during the 22 years was grassland, and farmland was the most secure land-use type. Overall, spatial analyses and examinations of residue disposal sites suggested that these locations have negatively impacted integrated land ecology since the beginning of the STR construction project. Our findings have implications for preserving the ecological ecosystem and ensuring the sustainability of the STR construction project.