Spatial analysis of multispecies bacterial biofilms

Guéneau, Virgile; Charron, Raphaël; Costache, Vlad; Bridier, Arnaud; Briandet, Romain

doi:10.1016/bs.mim.2023.03.002

Cited by 3 publications

(1 citation statement)

References 122 publications

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“…1,2 Within biofilms, bacteria can alter their genotypes and phenotypes, rearrange their spatial structures, and regulate their cooperative activities, developing unique community-intrinsic properties that are not observed in counterparts growing planktonically. 3,4 Because of these functional updates, however, biofilms are often detrimental, posing serious concerns to human health, water distribution systems, food and groceries, and industrial productivity. [5][6][7] Usually, bacteria densely packed in biofilms enhance virulence factors and become more infectious than planktonic cells.…”

Section: Introductionmentioning

confidence: 99%

Combined electrical-electrochemical phenotypic profiling of antibiotic susceptibility of in vitro biofilm models

Rafiee,

Rezaie,

Choi

2024

Analyst

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Section: Introductionmentioning

confidence: 99%

Combined electrical-electrochemical phenotypic profiling of antibiotic susceptibility of in vitro biofilm models

Rafiee,

Rezaie,

Choi

2024

Analyst

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Guided assembly of multispecies positive biofilms targeting undesirable bacteria

Guéneau,

Guillier,

Berdous

et al. 2024

Preprint

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The use of synthetic microbial communities (SynComs) engineered to form positive biofilms that prevent the settlement of harmful bacteria is emerging as a promising strategy in biotechnology, particularly in reducing reliance on chemical antimicrobials. Despite this potential, the rationale for selecting specific strains in SynComs and the mechanisms underlying their antagonistic effects remains insufficiently understood. In this study, we present a bottom-up approach integrating live-cell imaging with high-throughput analysis of multi-strain biofilms across diverse scenarios. Through this method, we identified beneficial strains based on their superior ability to exclude undesirable bacteria and form mixed biofilms. Notably, our findings revealed that competitive strains against undesirable bacteria could also exclude other beneficial strains, emphasising the need for compatibility control in SynComs design. SynComs composed of B. velezensis and Pediococcus spp. demonstrated enhanced pathogen exclusion compared to single strains. Temporal analysis of biofilm interactions, supported by mathematical models, showed that pathogen exclusion was primarily driven by nutritional competition (Jameson effect) with additional specific interference mechanisms (prey-predator Lotka-Volterra model). Furthermore, pre-establishing SynComs to surfaces significantly increased pathogen inhibition, indicating a distinct biofilm-associated exclusion effect. These insights offer a framework for rational SynCom design and deepen our understanding of the mechanisms underpinning positive biofilm applications.

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Light Control in Microbial Systems

Elahi,

Baker

2024

IJMS

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Light is a key environmental component influencing many biological processes, particularly in prokaryotes such as archaea and bacteria. Light control techniques have revolutionized precise manipulation at molecular and cellular levels in recent years. Bacteria, with adaptability and genetic tractability, are promising candidates for light control studies. This review investigates the mechanisms underlying light activation in bacteria and discusses recent advancements focusing on light control methods and techniques for controlling bacteria. We delve into the mechanisms by which bacteria sense and transduce light signals, including engineered photoreceptors and light-sensitive actuators, and various strategies employed to modulate gene expression, protein function, and bacterial motility. Furthermore, we highlight recent developments in light-integrated methods of controlling microbial responses, such as upconversion nanoparticles and optical tweezers, which can enhance the spatial and temporal control of bacteria and open new horizons for biomedical applications.

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Spatial analysis of multispecies bacterial biofilms

Cited by 3 publications

References 122 publications

Combined electrical-electrochemical phenotypic profiling of antibiotic susceptibility of in vitro biofilm models

Combined electrical-electrochemical phenotypic profiling of antibiotic susceptibility of in vitro biofilm models

Guided assembly of multispecies positive biofilms targeting undesirable bacteria

Light Control in Microbial Systems

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