Spatial and Temporal Distribution of Tie-1 and Tie-2 During Very Early Development of the Human Placenta

Kayisli, Umit A.; Çaylı, Sevil; Seval, Yasemin; Tertemiz, F.; Huppertz, Berthold; Demir, Ramazan

doi:10.1016/j.placenta.2005.05.013

Cited by 25 publications

(26 citation statements)

References 23 publications

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“…In the primate placenta, protein and/or mRNA expression of VEGF, FLT1, KDR, ANGPT1, ANGPT2, and/or receptor TEK was detected during early pregnancy (Demir et al 2004, Wei et al 2004, Seval et al 2008, Demir 2009). These factors appeared to be spatio-and temporal-regulated during early pregnancy in primates (Demir et al 2004, Wei et al 2004, Kayisli et al 2006, Seval et al 2008. The increased expression of several angiogenic factors during early pregnancy indicates that these factors are involved in the regulation of vascular development, remodeling, and trophoblast function.…”

Section: Discussionmentioning

confidence: 96%

Placental development during early pregnancy in sheep: cell proliferation, global methylation, and angiogenesis in the fetal placenta

Grazul-Bilska¹,

Johnson²,

Borowicz³

et al. 2011

Reproduction

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To characterize early fetal placental development, gravid uterine tissues were collected from pregnant ewes every other day from day 16 to 30 after mating. Determination of 1) cell proliferation was based on Ki67 protein immunodetection; 2) global methylation was based on 5-methyl-cytosine (5mC) expression and mRNA expression for DNA methyltransferases (DNMTs) 1, 3a, and 3b; and 3) vascular development was based on smooth muscle cell actin immunolocalization and on mRNA expression of several factors involved in the regulation of angiogenesis in fetal membranes (FMs). Throughout early pregnancy, the labeling index (proportion of proliferating cells) was very high (21%) and did not change. Expression of 5mC and mRNA for DNMT3b decreased, but mRNA for DNMT1 and 3a increased. Blood vessels were detected in FM on days 18-30 of pregnancy, and their number per tissue area did not change. The patterns of mRNA expression for placental growth factor, vascular endothelial growth factor, and their receptors FLT1 and KDR; angiopoietins 1 and 2 and their receptor TEK; endothelial nitric oxide synthase and the NO receptor GUCY13B; and hypoxia inducing factor 1 a changed in FM during early pregnancy. These data demonstrate high cellular proliferation rates, and changes in global methylation and mRNA expression of factors involved in the regulation of DNA methylation and angiogenesis in FM during early pregnancy. This description of cellular and molecular changes in FM during early pregnancy will provide the foundation for determining the basis of altered placental development in pregnancies compromised by environmental, genetic, or other factors.

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Section: Discussionmentioning

confidence: 96%

Placental development during early pregnancy in sheep: cell proliferation, global methylation, and angiogenesis in the fetal placenta

Grazul-Bilska¹,

Johnson²,

Borowicz³

et al. 2011

Reproduction

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“…Kayisli et al (30) reported that, in normal early pregnancy, vascular endothelial cells of the placental villi revealed weaker VEGF immunoreactivity than trophoblasts in all pregnancy weeks. They evaluated placental vasculogenesis in their study and they evaluated angiogenic proteins (Tie-1 and Tie-2) in the different stages of vasculogenesis related to cell type, villous maturation and pregnancy age during very early placental development (30). They also found that vascular endothelium Kutluer et al Defective angiogenesis in first-trimester miscarriages J Turkish-German Gynecol Assoc 2012; 13: 111-7 displayed a gradual decrease from strong to weak for Tie-2 immunoreactivity.…”

Section: Discussionmentioning

confidence: 99%

“…In the same study, the authors showed that, for both VEGF-A and its receptor VEGFR-2 (KDR/flk-1), this stage corresponded to the transition from secondary villi to tertiary villi, which also occurs at 21-32 days post conception (33). The soluble form of VEGF (VEGRF-1) and placental growth factor (PIGF) are expressed during later stages (branching angiogenesis phase), i.e., between 32 days and 25 weeks of gestation (28)(29)(30)(31)(32)(33). Therefore, the levels of serum (soluble) VEGF would not be suitable to predict early pregnancy loss.…”

Section: Discussionmentioning

confidence: 99%

Low VEGF expression in conceptus material and maternal serum AFP and levels as indicators of defective angiogenesis in first-trimester miscarriages

Kutluer¹,

Çiçek²,

Moraloğlu³

et al. 2012

J Turkish German Gynecol Assoc

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Low VEGF expression in conceptus material and maternal serum AFP and β-hCG levels as indicators of defective angiogenesis in first-trimester miscarriages Objective: The aims of this study were to assess the relationship between early miscarriages and vascular endothelial growth factor (VEGF) expression and to determine the serum levels of first-trimester maternal alpha-fetoprotein (AFP) and human chorionic gonadotropin (β-hCG) as markers of angiogenesis and predictors of abortion and intrauterine fetal loss. Material and Methods:The present study was a prospective, singlecenter, randomized controlled clinical trial. Ninety-five women who were 6-10 weeks pregnant between May and June 2010 were included in the study. The subjects were divided into three groups, i.e., incomplete abortion (IA) (n=31), intrauterine death (IU-D) (n=32) and control (elective pregnancy termination) (n=32). Feto-placental materials were compared based on immune staining for VEGF in the pathology laboratory, and maternal serum samples were tested in the hormone laboratory.Results: Serum β-hCG levels in the patient groups were significantly lower than the controls (p=0.001). The serum AFP level was lower than the controls in the IA group while it was higher than the controls in the IU-D (p=0.016). Immunohistochemistry showed that the cytotrophoblast, syncytiotrophoblast and endometrial gland epithelium were weakly stained for VEGF in the patient groups (IA and IU-D) in comparison to the control group (p=0.06, p=0.028, p=0.006). Conclusion:Early pregnancy losses are related to insufficient angiogenesis, and maternal serum AFP and β-hCG can be used as markers of angiogenesis in the first trimester.(J Turkish-German Gynecol Assoc 2012; 13: 111-7) Abstract ÖzetOriginal Investigation 111

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“…Western bloting procedures have been described in our recent studies (Demir et al, 2004;Kayisli et al, 2006) was applied. From placental tissues (3 samples for each gestational week; 8th week was addition for WB), n=15) total protein was extracted using a lysis buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 10% glycerol, 1% Triton X-100, 1.5 mM MgCl 2 , 1 mM EGTA, 100 mM NaF, 10 mM sodium pyrophosphate and protease inhibitors, 1mM Na 3 VO 4 , 10 mg/ml leupeptin, 10 mg/ml aprotinin, and 4 mM PMSF).…”

Section: Methodsmentioning

confidence: 99%

“…The vasculogenic importance of two newly detected receptors tryosine kinases Tie-1 and Tie-2, angiopoietin receptors, has been studied (Sato et al, 1995;Suri et al, 1996;Maisonpierre et al, 1997;Kayisli et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Expression of VEGF receptors VEFGR-1 and VEGFR-2, angiopoietin receptors Tie-1 and Tie-2 in chorionic villi tree during early pregnancy.

Demir

2010

Folia Histochem Cytobiol.

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Abstract:The aim of this study was to determine the expression of VEGF and its receptors in placentas from normal pregnancies between 22 days p.c. and 48 days p.c. of very early pregnancy. Placental tissues carried out from 19 pregnant women were examined. Immunohistochemical technique, electron microscopy were employed to evaluate the factors expression. In the new developing mesenchymal villi and immature intermediate villi VEGF and its receptors VEGFR-1 and VEGFR-2 immunoreactivity was detected in all the placental components, while in the stem villi and in the chorionic plate with large vessels only in some components. In the mesenchymal villi and immature intermediate villi VEGFR-1 and -2, and angiopoietin receptors Tie-1 and -2 immunoreactivity was dominantly observed in the heamangiogenic cells and cells cords, whereas the matured villi showed immunoreactivity only in other components. The ultrastructural findings were higher in respect to the all of the early pregnancy days. The placental samples from all of pregnancies, showed the VEGF and its receptors in optimal expression levels, whereas the angiopoietin receptors Tie-1 and -2 showed a higher expression levels in respect to other study factors. The receptors protein levels increased from the early days to the advanced days of gestation, but this alteration was not significant. The intensity of the immunolabeling for these proteins were not significant compared to to each other of gestatin days were examined. These findings demonstrated that a dysregulation of the placental expression of the VEGF and its receptors related to the different degrees of the gestational periods. Probably, this event may be related to complete vasculugenesis and angiogenesis in placental villi.

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Spatial and Temporal Distribution of Tie-1 and Tie-2 During Very Early Development of the Human Placenta

Cited by 25 publications

References 23 publications

Placental development during early pregnancy in sheep: cell proliferation, global methylation, and angiogenesis in the fetal placenta

Placental development during early pregnancy in sheep: cell proliferation, global methylation, and angiogenesis in the fetal placenta

Low VEGF expression in conceptus material and maternal serum AFP and levels as indicators of defective angiogenesis in first-trimester miscarriages

Expression of VEGF receptors VEFGR-1 and VEGFR-2, angiopoietin receptors Tie-1 and Tie-2 in chorionic villi tree during early pregnancy.

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