Spatial and temporal expression of c-Fos protein in the spinal cord of anesthetized rat induced by subcutaneous bee venom injection

Luo, Ceng; Chen, Jun; Li, Huili; Li, Ji-Shuo

doi:10.1016/s0006-8993(98)00721-5

Cited by 60 publications

(32 citation statements)

References 38 publications

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“…4B and 5). However, we observed far fewer FLI neurons as compared to the numbers reported by Luo et al [25] following intraplantar BV injection. This discrepancy is probably due to differences in the site of injection (subcutaneous versus intraplantar) and to the perfusion/fixation time.…”

Section: The Effect Of Bv Injection In Normal Animalscontrasting

confidence: 79%

“…Moreover, intraplantar BV injection induces Fos expression in the ipsilateral dorsal horn of the spinal cord and the spinal Fos expression induced by BV is attenuated by morphine treatment [25]. These data suggest that BV can be a useful agent not only for assessing the mechanisms of pain transmission, but also for evaluating the effectiveness of novel analgesic drugs in the treatment of tonic pain.…”

mentioning

confidence: 77%

“…Luo and his colleagues have reported that intraplantar injection of BV produced Fos expression in the L4-5 segments of the spinal cord [25]. These investigators indicated that maximal Fos expression occurred at 2 hr post-BV injection and that Fos expression lasted for 96 hr.…”

Section: The Effect Of Bv Injection In Normal Animalsmentioning

confidence: 98%

“…It has been recently reported that unilateral, subcutaneous injection of bee venom (BV) into the plantar surface of the hindpaw evokes prolonged and tonic behavioral responses resembling clinical persistent pain [6,23]. Moreover, intraplantar BV injection induces Fos expression in the ipsilateral dorsal horn of the spinal cord and the spinal Fos expression induced by BV is attenuated by morphine treatment [25]. These data suggest that BV can be a useful agent not only for assessing the mechanisms of pain transmission, but also for evaluating the effectiveness of novel analgesic drugs in the treatment of tonic pain.…”

mentioning

confidence: 99%

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Bee Venom Pretreatment Has Both an Antinociceptive and Anti-Inflammatory Effect on Carrageenan-Induced Inflammation.

et al. 2001

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ABSTRACT. Although the injection of bee venom (BV) has been reported to evoke tonic pain and hyperalgesia, there is conflicting evidence in the literature indicating that BV can also exert an anti-inflammatory and antinociceptive effects on inflammation. In this regard, BV has been traditionally used in Oriental medicine to relieve pain and to treat chronic inflammatory diseases such as rheumatoid arthritis. The present study was designed to test the hypothesis that BV induces acute nociception under normal conditions, but that it can serve as a potent anti-inflammatory and antinociceptive agent in a localized inflammatory state. The experiments were designed to evaluate the effect of BV pretreatment on carrageenan (CR)-induced acute paw edema and thermal hyperalgesia. In addition, spinal cord Fos expression induced by peripheral inflammation was quantitatively analyzed. In normal animals subcutaneous BV injection into the hindlimb was found to slightly increase Fos expression in the spinal cord without producing detectable nociceptive behaviors or hyperalgesia. In contrast pretreatment with BV (0.8 mg/kg) 30 min prior to CR injection suppressed both the paw edema and thermal hyperalgesia evoked by CR. In addition, there was a positive correlation between the percent change in paw volume and the expression of Fos positive neurons in the spinal cord. These results indicate that BV pretreatment has both antinociceptive and anti-inflammatory effects in CRinduced inflammatory pain. These data also suggest that BV administration may be useful in the treatment of the pain and edema associated with chronic inflammatory diseases. It has been recently reported that unilateral, subcutaneous injection of bee venom (BV) into the plantar surface of the hindpaw evokes prolonged and tonic behavioral responses resembling clinical persistent pain [6,23]. Moreover, intraplantar BV injection induces Fos expression in the ipsilateral dorsal horn of the spinal cord and the spinal Fos expression induced by BV is attenuated by morphine treatment [25]. These data suggest that BV can be a useful agent not only for assessing the mechanisms of pain transmission, but also for evaluating the effectiveness of novel analgesic drugs in the treatment of tonic pain. Although intraplantar BV injection can produce persistent pain under normal conditions, there are conflicting data indicating that BV can be both anti-inflammatory and antinociceptive under conditions of inflammation. In this regard it is interesting to note that BV therapy has been utilized as a traditional alternative medical approach to relieve pain and to treat inflammatory diseases such as rheumatoid arthritis in humans [1,3]. In experimental animals, adjuvant induced arthritis has been shown to be successfully suppressed by long-term BV treatment [8,12]. BV or its constituents have also been reported to be effective in the treatment of rheumatoid arthritis in humans [37]. Recently, we also demonstrated that that BV treatment produced antinociceptive and anti-inflammatory effect...

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Section: The Effect Of Bv Injection In Normal Animalscontrasting

confidence: 79%

mentioning

confidence: 77%

Section: The Effect Of Bv Injection In Normal Animalsmentioning

confidence: 98%

mentioning

confidence: 99%

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Bee Venom Pretreatment Has Both an Antinociceptive and Anti-Inflammatory Effect on Carrageenan-Induced Inflammation.

et al. 2001

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“…FOS-like immunoreactivity is considered a marker for central nociceptive neurons and is widely used for the investigation of somatotopy in nociceptive pathways [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Activation of Capsaicin Receptors on the Sciatic Nerve Induces FOS Expression in the Spinal Dorsal Horn of Adult Rats

Feng

Yang²,

Li³

2002

Neurosignals

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By using immunohistochemical staining for FOS protein in the spinal cord, the role of capsaicin receptors on the sciatic nerve was investigated. After topical application of capsaicin (1%) to the sciatic nerve, FOS-like immunoreactive (FOS-LI) neurons were observed, chiefly in the superficial laminae of the lumbar dorsal horn. Topical application of capsazepine (5%) or lidocaine (2%) to the sciatic nerve for 15 min before the application of capsaicin reduced the number of FOS-LI neurons in the superficial dorsal horn (by 83.2 ± 1.7 and 32.4 ± 1.2%, respectively). One week after pretreatment of the sciatic nerve with colchicine, the number of FOS-LI neurons induced by capsaicin was greatly decreased (by 74.6 ± 1.7%). Given that FOS protein expression after peripheral noxious stimulation is found in a location similar to that in the present study, our results indicate that the capsaicin receptor on the sciatic nerve is involved in the transmission of noxious information.

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Functional Organization of Dorsal Horn Interneurons

Willis¹,

Coggeshall²

2004

Sensory Mechanisms of the Spinal Cord

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Spatial and temporal expression of c-Fos protein in the spinal cord of anesthetized rat induced by subcutaneous bee venom injection

Cited by 60 publications

References 38 publications

Bee Venom Pretreatment Has Both an Antinociceptive and Anti-Inflammatory Effect on Carrageenan-Induced Inflammation.

Bee Venom Pretreatment Has Both an Antinociceptive and Anti-Inflammatory Effect on Carrageenan-Induced Inflammation.

Activation of Capsaicin Receptors on the Sciatic Nerve Induces FOS Expression in the Spinal Dorsal Horn of Adult Rats

Functional Organization of Dorsal Horn Interneurons

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