Spatial and Temporal Expression of Abscission-Related Genes During Ethylene-Promoted Organ Shedding

Temporal and spatial expression of mRNAs encoding pathogenesis‐related proteins during ethylene‐promoted leaflet abscission in Sambucus nigra*

“…Abscission of the leallets of the herbaceous plant Sanihtictis nigra has proved to be an effective model system for the study of abscission, as the zone of sepatation is made up of a sufficient number of rows of cells to facilitate the extraction of proteins and nucleic acids. S(udies on this plant have revealed that during ethylene-stitnulated leaflet abscission (he activities of both polygalacturonase and /3-1,4-glucanase increase specifically at the site of separation Webb et al 1993) and that this is correlated with an inctease in the expression of genes encoding these etizytnes (Taylor et al 1994;Roberts et al 1997). Additional ptoteins have been teported to be produced dut--ing leaf abscission in other species (Kelly et al 1987;Gomez Lim et al 1987).…”

A structurally divergent 1-aminocyclopropane-1-carboxylate synthase gene family coordinates abscission, wounding, and auxin responses in soybean

“…External factors that differentially control expression of specific ACC-synthase gene family members include auxin treatment, chilling injury, wounding, flooding, and elicitors Tsuchisaka and Theologis, 2004; Yang and Hoffman, 1984). It is curious that the regulation of ACC synthases has not been studied during organ abscission, although ethylene is known to accelerate the shedding of both vegetative and reproductive structures Reid, 1985; Roberts et al, 1997;Taylor and Whitelaw, 2001). In a number of agriculturally important plants including soybean and cotton, 50-80~Io or more of reproductive parts are routinely aborted or abscised prior to harvest, seriously reducing yield potential (Peterson et al, 1992;Guinn, 1982).…”

“…There are a number of important physiological processes regulated by ethylene in which no studies of ACC synthase regulation have been performed. For example, the regulation of ACC synthases has not been studied during organ abscission, although ethylene accelerates the shedding of both vegetative and reproduction structures Reid, 1985 ;Roberts et al, 1997;Taylor and Whitelaw, 2001) and endogenous ethylene production near the abscission layer increases in abscising organs Reid, 1985).…”

Aproximación genómica al estudio de la abscisión de frutos cítricos: análisis transcriptómico de la zona de abscisión C

“…Esta es la razón por la que a medida que se desarrolla el proceso de abscisión, la planta activa una serie de medidas preventivas destinadas a protegerse ante estos ataques potenciales. Diversos trabajos han documentado 40 la inducción de la síntesis de proteínas de defensa como las quitinasas, las β-1,3-glucanasas (del Campillo y Lewis, 1992) y otras como las PR, de respuesta a patógenos, y las WIN, inducibles por herida durante la abscisión (Harris et al, 1997;Roberts et al, 1997). La coordinación de la expresión de estas proteínas durante el proceso de abscisión es incierta, aunque podría estar relacionada con la presencia de intermediarios reactivos del oxígeno (Lamb, 1994;Roberts et al, 2002).…”