Spatial and Temporal Expression of the Sperm Protein SPRASA in Mice.

Prendergast, Deborah; Woad, Kathryn J.; Chamley, Larry; Shelling, Andrew N.

doi:10.1093/biolreprod/78.s1.301a

Cited by 1 publication

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“…Furthermore, we have found mRNA expression of SPRASA in murine ovaries. 31 Other proteins originally thought to be sperm specific such as spermadhesin and PH-20 have been shown to be expressed in both male and female reproductive tracts and to have potential roles in reproduction in both sexes. 32,33 The function of SPRASA on the oocyte is unclear, but it seems likely that this role is additional to sperm-oocyte interactions.…”

Section: Function Of Sprasa and Spermmentioning

confidence: 99%

The Role of SPRASA in Female Fertility

et al. 2015

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Fertility is a complex process and infertility can have many causes. Sperm protein reactive with antisperm antibody (SPRASA)/sperm lysozyme-like protein 1 is a protein discovered as the target of autoantibodies in infertile men and previously thought to be expressed only in sperm. Using a bovine in vitro fertilization model, we have shown that SPRASA antiserum reduced sperm binding to zona-free oocytes and the development of embryos to morulae but did not affect the postfertilization cleavage rate to 2 cells or sperm motility. We demonstrated that SPRASA was expressed in ovarian follicles, corpora lutea, and oocytes by a combination of reverse transcription-polymerase chain reaction and immunohistochemistry. Female mice immunized with SPRASA had profound infertility following timed matings and those mice that did become pregnant had reduced fetal viability. The levels of antibodies reactive with SPRASA in 204 fertile and 202 infertile couples were elevated in 3 infertile but no fertile women. Together, these results indicate that SPRASA has a role in female fertility.

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Section: Function Of Sprasa and Spermmentioning

confidence: 99%