Spatial and temporal expressions of two distinct oleate desaturases from olive (Olea europaea L.)

Molecular cloning and characterization of a novel microsomal oleate desaturase gene from soybean

“…Unlike reports by Hernandez et al (2005), the position of FAD6 was closer to Housekeeping-type FAD2 than that of the Seed-type FAD2. These observations supported the hypothesis that diverged FAD2 enzymes with the same functionality, but not forming the same clade, may arise independently several times during evolution, and the evolutionary process of Housekeeping-type FAD2, FAD6 and Seed-type FAD2 genes might be different from each other (Dyer et al, 2002;Banilas et al, 2005). In addition, the observed relative high level of divergence within the Housekeeping-type FAD2 group implied that certain genes might have evolved differently, although more data are required to further confirm this observation.…”

Cloning and expression analysis of three cDNAs encoding omega-3 fatty acid desaturases from Descurainia sophia

“…The FAD7 gene was also expressed in the flower apparatus of O. europaea (Zaruhi et al 1999). It is most likely to furnish the demand of polyunsaturated fatty acids in membrane biosynthesis (Banilas et al 2005). Expression of DsFAD3, DsFAD7 and DsFAD8 in leaves under wounding stress showed that transcript levels of DsFAD3 and DsFAD8 were slowly increased at 3 h after treatment, reached a maximum level at 6 h, DsFAD8 then dramatically declined and DsFAD3 remained at a stable level for 24 h. However, the expression of DsFAD7 rapidly increased at 1 h after treatment and reached a maximum level at 3 h and then slowly declined to the level before treatment (Fig.…”

Effect of different environmental stresses on the expression of oleate desaturase genes and fatty acid composition in olive fruit

“…In olive, two genes encoding microsomal oleate desaturases (OepFAD2-1 and OepFAD2-2) have been reported (Hernández et al, 2005), whereas only one gene corresponding to the plastidial oleate desaturase (OeFAD6) has been described (Banilas et al, 2005), but without confirming its functional identity. Expression analysis of these genes revealed that they are spatially and temporally regulated.…”