Spatial asynchronous transfer of cleavage‐stage mouse embryos to the uterus compromises fetal development

Walker, Kathryn J; Green, Mark P.

doi:10.1002/mrd.22456

Cited by 9 publications

(2 citation statements)

References 3 publications

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“…What are the reasons/advantages for blastocyst transfer in the first place, and do they remain valid? In all mammalian species studied to date the asynchronous spatial transfer of cleavage-stage embryos to the uterus compromises resultant fetal development (Barnes, 2000;Walker et al, 2015), an observation not commonly acknowledged by the clinical IVF community. Hence, the development of more suitable and physiological culture systems that could support the development of viable human blastocysts was undertaken within the context of synchronicity, in order to increase implantation and live birth rates and to facilitate the move to single embryo transfer (Gardner and Lane, 2003).…”

Section: Do We Really Need To Offer Blastocyst Transfer?mentioning

confidence: 99%

The impact of physiological oxygen during culture, and vitrification for cryopreservation, on the outcome of extended culture in human IVF

Gardner

2016

Reproductive BioMedicine Online

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Extended culture has facilitated the move to single blastocyst transfer, resulting in significant increases in implantation and live birth rate, while concomitantly reducing fetal loss during pregnancy. However, concerns have been raised regarding subsequent neo-natal outcomes following extended culture. Analysis of the literature reveals differences in outcomes according to geographical region and between individual clinics. A common factor amongst reports of potentially adverse outcomes following blastocyst transfer appears to be that atmospheric (~20%) oxygen was typically employed for embryo culture. Clinics and countries utilizing physiological concentrations of oxygen (~5%) have not reported adverse perinatal outcomes with blastocyst transfer. Atmospheric oxygen imposes significant negative effects upon the embryo's molecular and cellular physiology, and further it increases the sensitivity of the preimplantation embryo to other stressors in the laboratory. With the recent adoption of vitrification for blastocyst cryopreservation, cumulative pregnancy rates per cycle with extended culture will increase significantly. Consequently, rather than perceiving extended culture as a potentially negative procedure, it is concluded that neo-natal data need to be interpreted in light of the conditions used to culture and cryopreserve blastocysts, and that furthermore a policy of embryo culture using 20% oxygen can no longer be justified.

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Section: Do We Really Need To Offer Blastocyst Transfer?mentioning

confidence: 99%

The impact of physiological oxygen during culture, and vitrification for cryopreservation, on the outcome of extended culture in human IVF

Gardner

2016

Reproductive BioMedicine Online

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“…98 With the advent of blastocyst culture the discussion emerged as to whether it was better to replace the human embryo into the uterus as early as possible (thereby avoiding several of the issues raised herein associated with development in vitro) or transfer the embryo to the uterus at the stage and time it would normally reside there, that is the blastocyst, which is the most effective stage for transfer in animal models. 280,281 After 20 years of clinical blastocyst transfer there is considerable evidence that blastocyst transfer can be more successful than cleavage stage transfer. However, findings have not been universal and this is likely due to the interactions between components of the culture system, the types of incubation system, training levels and number of embryologists, as well as investment in quality control.…”

Section: Asynchronous Transfer 'Culture In Vivo'mentioning

confidence: 99%

Impact of the IVF laboratory environment on human preimplantation embryo phenotype

Gardner

Kelley

2017

J Dev Orig Health Dis

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The phenotype of the human embryo conceived through in vitro fertilization (IVF), that is its morphology, developmental kinetics, physiology and metabolism, can be affected by numerous components of the laboratory and embryo culture system (which comprise the laboratory environment). The culture media formulation is important in determining embryo phenotype, but this exists within a culture system that includes oxygen, temperature, pH and whether an embryo is cultured individually or in a group, all of which can influence embryo development. Significantly, exposure of an embryo to one suboptimal component of the culture system of laboratory typically predisposes the embryo to become more vulnerable to a second stressor, as has been well documented for atmospheric oxygen and individual culture, as well as for oxygen and ammonium. Furthermore, the inherent viability of the human embryo is derived from the quality of the gametes from which it is created. Patient age, aetiology, genetics, lifestyle (as well as ovarian stimulation in women) are all known to affect the developmental potential of gametes and hence the embryo. Thus, as well as considering the impact of the IVF laboratory environment, one needs to be aware of the status of the infertile couple, as this impacts how their gametes and embryos will respond to an in vitro environment. Although far from straight forward, analysing the interactions that exist between the human embryo and its environment will facilitate the creation of more effective and safer treatments for the infertile couple.

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Sequential Media for Human Blastocyst Culture

Lane

2019

In Vitro Fertilization

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Spatial asynchronous transfer of cleavage‐stage mouse embryos to the uterus compromises fetal development

Cited by 9 publications

References 3 publications

The impact of physiological oxygen during culture, and vitrification for cryopreservation, on the outcome of extended culture in human IVF

The impact of physiological oxygen during culture, and vitrification for cryopreservation, on the outcome of extended culture in human IVF

Impact of the IVF laboratory environment on human preimplantation embryo phenotype

Sequential Media for Human Blastocyst Culture

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