Spatial concentration distribution analysis of cells in electrode-multilayered microchannel by dielectric property measurement

Yao, Jiafeng; Kodera, Tatsuya; Obara, Hiromichi; Sugawara, Michiko; Takei, Masahiro

doi:10.1063/1.4929824

Cited by 21 publications

(11 citation statements)

References 37 publications

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“…The first EIT‐based lung image was generated by measuring the potential difference on the human chest. The EIT technology is used in many fields due to its unique advantages, such as the Earth exploration, industrial applications, biomedical imaging, and robotic applications based on artificial sensitive skins in recent years …”

Section: Introductionmentioning

confidence: 99%

Artificial Sensitive Skin for Robotics Based on Electrical Impedance Tomography

Wang

et al. 2020

Advanced Intelligent Systems

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Electrical impedance tomography (EIT) is a noninvasive measurement technique that estimates the internal resistivity distribution based on the boundary voltage–current data measured from the surface of the conductor. If a thin stretchable soft material with a certain piezoresistive property is used as the electrical conductor, EIT has the ability to reconstruct the position where the resistivity changes due to the inside pressure contact, so that a large‐scale artificial sensitive skin is provided for robotics. First, the different conduction principles and material types of artificial sensitive skins are discussed, which is next followed by the different driving modes and image reconstruction techniques. Then, details on how EIT is used for robotic skin applications are described. Finally, the development trends and future potentials of EIT‐based robotic skins are expounded.

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Section: Introductionmentioning

confidence: 99%

Artificial Sensitive Skin for Robotics Based on Electrical Impedance Tomography

Wang

et al. 2020

Advanced Intelligent Systems

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“…In order to extract the particle concentration from the measured capacitance, the MES approach similar to our previous study is employed. 16 In brief, the capacitance data measured by the capacitance acquisition unit are first normalized by a calibration process to remove the stray capacitance and residual inductances inherent to the cables and electrical leads. The local particle concentration a p in the square microchannel are then calculated at the two sensing planes by the modified Maxwell model as…”

Section: B Experimental Conditions and Methodsmentioning

confidence: 99%

“…In order to overcome the difficulty of the conventional methods to evaluate the particle inertial migration in the high concentration solution, the multi-electrodes sensing (MES) method had been proposed and been successfully applied in the measurement of cell concentration in concentrated suspensions (up to a pi ¼ 40.0 v/v%). 16 In this study, online measurement in a square microchannel under a range of high initial particle concentration (from a pi ¼ 3.0 to 40.0 v/v%) by using MES method is demonstrated to clarify the effect of initial particle concentration on particle inertial migration. In order to quantitatively evaluate the degree of particle inertial migration, cross-sectional particle inertial migration ratios are determined by measured particle concentration near the wall and at the corner of the square microchannel.…”

Section: Introductionmentioning

confidence: 99%

Investigation of particle inertial migration in high particle concentration suspension flow by multi-electrodes sensing and Eulerian-Lagrangian simulation in a square microchannel

Zhao

Yao

et al. 2016

Biomicrofluidics

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The inertial migration of neutrally buoyant spherical particles in high particle concentration (αpi > 3%) suspension flow in a square microchannel was investigated by means of the multi-electrodes sensing method which broke through the limitation of conventional optical measurement techniques in the high particle concentration suspensions due to interference from the large particle numbers. Based on the measured particle concentrations near the wall and at the corner of the square microchannel, particle cross-sectional migration ratios are calculated to quantitatively estimate the migration degree. As a result, particle migration to four stable equilibrium positions near the centre of each face of the square microchannel is found only in the cases of low initial particle concentration up to 5.0 v/v%, while the migration phenomenon becomes partial as the initial particle concentration achieves 10.0 v/v% and disappears in the cases of the initial particle concentration αpi ≥ 15%. In order to clarify the influential mechanism of particle-particle interaction on particle migration, an Eulerian-Lagrangian numerical model was proposed by employing the Lennard-Jones potential as the inter-particle potential, while the inertial lift coefficient is calculated by a pre-processed semi-analytical simulation. Moreover, based on the experimental and simulation results, a dimensionless number named migration index was proposed to evaluate the influence of the initial particle concentration on the particle migration phenomenon. The migration index less than 0.1 is found to denote obvious particle inertial migration, while a larger migration index denotes the absence of it. This index is helpful for estimation of the maximum initial particle concentration for the design of inertial microfluidic devices.

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“…The specific operation is like this: with different combinations of the excitation multilayered electrodes, different types of electric fields are generated, 25 correspond to various electrokinetic forces in 2 dimensions; furthermore, 5 cross-sections are used to manipulate the cells in the 3rd dimension. In the present experiment, as a first step to understand the particles' movement in high conductive fluid and to estimate the dominant forces exerting on the particles in the 3-DIMES, 2 electrodes are excited to understand the particles' movement in the microchannel, which provides a fundamental test for 3D manipulation in the next study.…”

Section: Concept and Fabrication Of The 3-dimesmentioning

confidence: 99%

“…Currently, a multilayered electrodes microchannel with diamond cross-sections was fabricated by our laboratory 24 to measure the concentration distribution of cells in the microchannel; 25 however, the flow characteristics of particles in the multilayered electrodes microchannel were not fully understood because the diamond cross-sections block optic to measure the particles' movement. Therefore, a new type of cross-sections to transparent optics is totally necessary.…”

Section: Introductionmentioning

confidence: 99%

Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics

et al. 2016

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An optical transparent 3-D Integrated Microchannel-Electrode System (3-DIMES) has been developed to understand the particles' movement with electrokinetics in the microchannel. In this system, 40 multilayered electrodes are embedded at the 2 opposite sides along the 5 square cross-sections of the microchannel by using Micro Electro-Mechanical Systems technology in order to achieve the optical transparency at the other 2 opposite sides. The concept of the 3-DIMES is that the particles are driven by electrokinetic forces which are dielectrophoretic force, thermal buoyancy, electrothermal force, and electroosmotic force in a three-dimensional scope by selecting the excitation multilayered electrodes. As a first step to understand the particles' movement driven by electrokinetic forces in high conductive fluid (phosphate buffer saline (PBS)) with the 3-DIMES, the velocities of particles' movement with one pair of the electrodes are measured three dimensionally by Particle Image Velocimetry technique in PBS; meanwhile, low conductive fluid (deionized water) is used as a reference. Then, the particles' movement driven by the electrokinetic forces is discussed theoretically to estimate dominant forces exerting on the particles. Finally, from the theoretical estimation, the particles' movement mainly results from the dominant forces which are thermal buoyancy and electrothermal force, while the velocity vortex formed at the 2 edges of the electrodes is because of the electroosmotic force. The conclusions suggest that the 3-DIMES with PBS as high conductive fluid helps to understand the threedimensional advantageous flow structures for cell manipulation in biomedical applications. V C 2016 AIP Publishing LLC. [http://dx

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Spatial concentration distribution analysis of cells in electrode-multilayered microchannel by dielectric property measurement

Cited by 21 publications

References 37 publications

Artificial Sensitive Skin for Robotics Based on Electrical Impedance Tomography

Artificial Sensitive Skin for Robotics Based on Electrical Impedance Tomography

Investigation of particle inertial migration in high particle concentration suspension flow by multi-electrodes sensing and Eulerian-Lagrangian simulation in a square microchannel

Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics

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