Spatial control of cells, peptide delivery and dynamic monitoring of cellular physiology with chitosan-assisted dual color quantum dot FRET peptides

Fu, Ru Huei; Liu, Shih‐Ping; Ou, Chen Wei; Huang, Chin Mao; Wang, Yu Chi

doi:10.1016/j.actbio.2010.03.005

Cited by 5 publications

(2 citation statements)

References 58 publications

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“…Recent reports have shown that incorporation of phytocompounds into liposome or nanoparticles improves in the oral delivery of a drug [94]–[96], as these particles can avoid it degradation in the gastrointestinal tract; as a result of their individual absorption mechanism through the circulation system, these particles also shield the drug from the first-pass effect in the liver and allow continued release at the correct site of action. Such techniques will be exploited for improvements in the clinical applications of n -butylidenephthalide.…”

Section: Discussionmentioning

confidence: 99%

n-Butylidenephthalide Protects against Dopaminergic Neuron Degeneration and α-Synuclein Accumulation in Caenorhabditis elegans Models of Parkinson's Disease

Harn

Liu

et al. 2014

PLoS ONE

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BackgroundParkinson's disease (PD) is the second most common degenerative disorder of the central nervous system that impairs motor skills and cognitive function. To date, the disease has no effective therapies. The identification of new drugs that provide benefit in arresting the decline seen in PD patients is the focus of much recent study. However, the lengthy time frame for the progression of neurodegeneration in PD increases both the time and cost of examining potential therapeutic compounds in mammalian models. An alternative is to first evaluate the efficacy of compounds in Caenorhabditis elegans models, which reduces examination time from months to days. n-Butylidenephthalide is the naturally-occurring component derived from the chloroform extract of Angelica sinensis. It has been shown to have anti-tumor and anti-inflammatory properties, but no reports have yet described the effects of n-butylidenephthalide on PD. The aim of this study was to assess the potential for n-butylidenephthalide to improve PD in C. elegans models.Methodology/Principal FindingsIn the current study, we employed a pharmacological strain that expresses green fluorescent protein specifically in dopaminergic neurons (BZ555) and a transgenic strain that expresses human α-synuclein in muscle cells (OW13) to investigate the antiparkinsonian activities of n-butylidenephthalide. Our results demonstrate that in PD animal models, n-butylidenephthalide significantly attenuates dopaminergic neuron degeneration induced by 6-hydroxydopamine; reduces α-synuclein accumulation; recovers lipid content, food-sensing behavior, and dopamine levels; and prolongs life-span of 6-hydroxydopamine treatment, thus revealing its potential as a possible antiparkinsonian drug. n-Butylidenephthalide may exert its effects by blocking egl-1 expression to inhibit apoptosis pathways and by raising rpn-6 expression to enhance the activity of proteasomes.Conclusions/Significance n-Butylidenephthalide may be one of the effective neuroprotective agents for PD.

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Section: Discussionmentioning

confidence: 99%

n-Butylidenephthalide Protects against Dopaminergic Neuron Degeneration and α-Synuclein Accumulation in Caenorhabditis elegans Models of Parkinson's Disease

Harn

Liu

et al. 2014

PLoS ONE

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“…Chitosan (CTS), with a repeating structure unit of β‐(1, 4)‐2‐amino‐2‐deoxy‐β‐ d ‐glucose, is a fully or partly deacetylated product of chitin, which is an abundant natural resource . Because of its biodegradability, nontoxicity, and antimicrobial activity, CTS has been widely used for biomedical applications, such as tissue engineering scaffolds, drug delivery, wound dressings, and antibacterial coatings . However, the existence of strong intramolecular and intermolecular hydrogen bonds between hydroxyl groups and amino groups makes it insoluble in most solvents.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and antibacterial activity of alkylated chitosan under basic ionic liquid conditions

Pei

Cai

Shang

et al. 2013

J of Applied Polymer Sci

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A novel method to synthesize alkylated chitosan with [Bmim]OH basic ionic liquid as alkaline reagent is reported for the first time. Chitosan was alkalized by [Bmim]OH basic ionic liquid before it was reacted with various alkyl halides, and a series of alkylated chitosans with different carbon chain substituents were prepared. The structure of alkylated product was characterized by IR, 1 H-NMR, and XRD. The reusability of the ionic liquid was also investigated, and the experimental results indicated that there was no noticeable change for the degree of substitution (DS) of alkylated chitosan after the ionic liquid was reused for three times. The antibacterial activities of the target products were investigated according their minimum inhibitory concentration (MIC) against K. pneumoniae, P. aeruginosa, E. aerogenes, E. coil, S. epidermidis, and S. aureus, and the results indicated that E-CTS, B-CTS, and D-CTS could exhibit excellent antibacterial activities against P. aeruginosa, and their MIC were 128, 128, and 256 lg mL 21 , respectively. Meanwhile, the antibacterial activities of alkylted chitosans were decreased with the growth of the DS or the growth of the carbon chain.

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Byrne¹,

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Coker³

et al. 2013

FRET – Förster Resonance Energy Transfer

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Spatial control of cells, peptide delivery and dynamic monitoring of cellular physiology with chitosan-assisted dual color quantum dot FRET peptides

Cited by 5 publications

References 58 publications

n-Butylidenephthalide Protects against Dopaminergic Neuron Degeneration and α-Synuclein Accumulation in Caenorhabditis elegans Models of Parkinson's Disease

n-Butylidenephthalide Protects against Dopaminergic Neuron Degeneration and α-Synuclein Accumulation in Caenorhabditis elegans Models of Parkinson's Disease

Synthesis and antibacterial activity of alkylated chitosan under basic ionic liquid conditions

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