Spatial control over cell attachment by partial solvent entrapment of poly lysine in microfluidic channels

Baman, Nicki K; Schneider, Galen B.; Terry, Treniece L.; Zaharias, Rebecca; Salem, Aliasger K.

doi:10.2147/nano.2006.1.2.213

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Figure also shows that HEPM cells attached to the RGD surface engineered substrates were spreading well with healthy cytoskeletal structure and morphology by 72 h. Control hydrogel samples prepared from PEGDMA alone resulted in negligible cell attachment and cells attached to the substrate by 72 h had not spread well and were predominantly rounded and dying. This is consistent with previous observations from us and others that PEG significantly reduces cell attachment because of PEGs protein resistance properties. – …”

Section: Resultssupporting

confidence: 94%

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Biotinylated Biodegradable Nanotemplated Hydrogel Networks for Cell Interactive Applications

et al. 2008

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We describe the synthesis of a novel biotinylated nanotextured degradable hydrogel that can be rapidly surface engineered with a diverse range of biotinylated moieties. The hydrogel is synthesized by reacting methacrylated biotin-PEG with dimethacrylated P LA-b- PEG-b-P LA (LPLDMA, PEG = poly(ethylene glycol), PLA = poly(lactic acid)),or dimethacrylated PEG-b-P LA-b- PEG (PLPDMA). Methacrylated biotin-PEG is prepared by reacting biotin-PEG-OH with methacrylic anhydride. Biotin-PEG-OH is prepared by reacting alpha-hydroxy-omega-amine PEG with N-hydroxysuccinimide-biotin. Confirmation of the final product is determined using (1)H NMR and Fourier transform infrared spectroscopy (FTIR). The integrity and surface presentation of the biotin units is observed spectrophotometrically using the HABA/avidin assay. To produce nanostructured polymer topography, a self-assembling lyotropic liquid crystalline mesophase is used as a polymerization template, generating biotinylated hydrogels with highly organized lamellar matrix geometry. Traditionally processed isotropic hydrogels are used for comparison. Scanning electron microscopy shows that isotropic hydrogels have a smooth glassy appearance while lamellar templated hydrogels have defined surface topographical features that enhance preosteoblast human palatal mesenchymal cell (HEPM) attachment. Engineering the surfaces of the hydrogels with cell adhesive Arg-Gly-Asp (RGD) peptide sequences using the biotin-avidin interaction significantly enhances cell attachment. Surface engineering of cell adhesive peptides in conjunction with the lamellar template induced surface topography generates additive enhancements in cell attachment.

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Section: Resultssupporting

confidence: 94%

“…This is consistent with previous observations from us and others that PEG significantly reduces cell attachment because of PEGs protein resistance properties. [40][41][42][43]…”

Section: Resultsmentioning

confidence: 99%

Biotinylated Biodegradable Nanotemplated Hydrogel Networks for Cell Interactive Applications

et al. 2008

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“…The adhesion of human gingival fibroblast cells (HGF, obtained from Provitro, GmbH, Germany) on SCS–DAS hybrid hydrogel surface was assessed. The separated solutions of both SCS and DAS in phosphate buffers were first sterilized under UV for 30 min [27] . The last solutions were mixed together according to the aforementioned studied ratios of SCS and DAS into the 48-well culture plate, and then followed with incubation at 37 °C for 2 h, to ensure formation of all hybrid hydrogels.…”

Section: Methodsmentioning

confidence: 99%

N-succinyl chitosan–dialdehyde starch hybrid hydrogels for biomedical applications

Kamoun

2016

Journal of Advanced Research

113

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A new class of injectable, biocompatible and biodegradable hydrogel is reported. This hydrogel is derived from N-succinyl chitosan (SCS) mixed with water-soluble dialdehyde starch (DAS) without using a conventional chemical crosslinker. The hybrid hydrogel is formed owing to the Schiff’s base reaction between amine groups of SCS and dialdehyde groups of DAS to form —CHN— group. SCS, DAS, and SCS–DAS hybrid hydrogels were synthesized and then characterized by FTIR analysis spectroscopy. The influence of SCS:DAS ratio in hybrid polymers solution on physicochemical properties of resultant hydrogels (e.g. gelation time, gel fraction (%) and equilibrium swelling ratio), surface morphology, in vitro weight loss (%), and mechanical stability was examined. The results demonstrated that SCS content has a profound role for forming tighter crosslinked hybrid hydrogels, where the increase of SCS content reduces the time for hydrogel forming. Also, the water uptake and hydrolytic weight loss decrease. Meanwhile, the DAS content increases, and mechanical properties of SCS–DAS hybrid hydrogels decrease. Curcumin release profile and adhered HGF cells on hydrogel surface sharply influenced the SCS portion in hybrid hydrogel composition. The SCS–DAS hybrid hydrogel properties afforded a possible opportunity to be used as a covalent in situ forming hybrid hydrogels in biomedical applications such as, tissue engineering and cartilage repair.

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“…resolutions on various nano-surfaces. Such protein-coated surfaces have been widely used for the attachment and confined growth of neurons as well as myocytes, 32 fibroblasts, 33 mesenchymal cells, 34 and Hela cells 35 in an in vitro environment. In this work, we report our results on the use of PDL on neuronal growth.…”

Section: Introductionmentioning

confidence: 99%

Growth of E18 Rat Hippocampal Neuronal Cells on Nanostructured Surfaces for Enhanced Electrical Stimulation and Recording

Islam

Panaitescu²,

Sinno³

et al. 2012

j nanosci nanotechnol

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We have prepared gold nanowire arrays inside nanoporous alumina templates with the goal towards neuronal interfacing and electrical recording from neurons. We have investigated biofunctionalization of such gold nanowire arrays (GNWs) and gold nanofilm (GNF) platforms to understand its impact on neuronal attachment and growth. Poly-D-Lysine (PDL) was coated on the nano-templates surfaces for adhesion of neurons which also enhanced the neuronal growth. Optical microscopy and scanning electron microscopy images revealed strong affinity and improved growth of neurons on PDL-coated surfaces. Such results will impact future investigation of stimulation and recording of electrical activity on nanoscale surfaces.

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Spatial control over cell attachment by partial solvent entrapment of poly lysine in microfluidic channels

Cited by 6 publications

References 25 publications

Biotinylated Biodegradable Nanotemplated Hydrogel Networks for Cell Interactive Applications

Biotinylated Biodegradable Nanotemplated Hydrogel Networks for Cell Interactive Applications

N-succinyl chitosan–dialdehyde starch hybrid hydrogels for biomedical applications

Growth of E18 Rat Hippocampal Neuronal Cells on Nanostructured Surfaces for Enhanced Electrical Stimulation and Recording

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