Spatial determination of elements in green leaves of oak trees (Quercus robur) by laser ablation-ICP-MS

Hoffmann, Erwin; Lüdke, C.; Skole, J.; Stephanowitz, Heike; Ullrich, E.; Colditz, D.

doi:10.1007/s002160000429

Cited by 44 publications

(36 citation statements)

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“…The spectroscopic laser ablation method has been used to find spatial patterns in cation concentrations in green oak leaves (Hoffmann et al 2000). Our results confirm that good sensitivity and fine spatial resolution, combined with the ability to sample growing tissue with minimal disruption to surrounding tissue (Fig.…”

Section: La-icp-ms As a Tool For Studying Metals In Growing Tissuessupporting

confidence: 83%

Laser ablation ICP-MS reveals patterns of copper differing from zinc in growth zones of cucumber roots

Shi

Gras²,

Silk

2009

Planta

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Laser ablation coupled with inductively coupled plasma-mass spectrometry was used to find Cu and Zn concentration in surface tissue along a longitudinal developmental gradient with meristem, rapidly elongating tissue, and nongrowing tissue in a model system of seedling roots of Cucumis sativus L. (cucumber). Tissue metal accumulation was determined for roots of seedlings growing on cellulosic germination paper treated with nutrient solution (controls), and also treated with concentrations of Zn (40 ppm) and Cu (10 ppm) that reduced growth. Cu content of all roots is highest at the apex and falls sharply to lower values by 2 mm from the root tip. In contrast, at moderate Zn availability (0.07 ppm), Zn content rises from the apex to 2 mm then falls throughout the remainder of the growth zone. At high external Zn the spatial pattern resembles that of Cu. Cucumber root growth zones accumulate more of each metal with higher external availability. Metal deposition rates were calculated using a continuity equation with data on local metal content and growth velocity. Deposition rates of both metals are generally highest in the rapidly elongating region, 1.5-3.5 mm, even where metal concentration is decreasing with position and root age and even when the accumulation is inhibitory to growth.

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Section: La-icp-ms As a Tool For Studying Metals In Growing Tissuessupporting

confidence: 83%

Laser ablation ICP-MS reveals patterns of copper differing from zinc in growth zones of cucumber roots

Shi

Gras²,

Silk

2009

Planta

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“…www.rsc.org/jaas for quantification purposes in LA-ICP-MS using a preparation of matrix-matched laboratory standards or by solution-based calibration. Due to these advantages, LA-ICP-MS, which is mostly used as a trace analytical technique for the bulk analysis of solid inorganic matrices and partly for isotope ratio measurements, [16][17][18][19] will also be applied to an increasing extent for element distribution analysis (including analysis of trace elements) in biological samples and medical tissues (e.g., in tree barks, 20 green leaves, 21 fish 22 or liver tissue 13 ). In addition, LA-ICP-MS was applied as a microlocal analytical method for the determination of P, S, Si and metal concentrations (Al, Zn, Cu und Fe) in well-separated protein spots after two-dimensional gel electrophoresis in brain samples of patients with Alzheimer's disease and in control brains.…”

Section: A R T I C L Ementioning

confidence: 99%

Copper, zinc, phosphorus and sulfur distribution in thin section of rat brain tissues measured by laser ablation inductively coupled plasma mass spectrometry: possibility for small-size tumor analysis

Becker¹,

Zoriy²,

Dehnhardt

et al. 2005

J. Anal. At. Spectrom.

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A microanalytical method using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was developed to measure element distribution in rat brain tissues for the detection of a small-size tumor. The stereotaxically guided tumor was implanted by injecting 5 ml of 10 3 F98 cells into the right Caudatus putamen of a male F344 Fisher rat brain hemisphere. The second non-treated rat brain hemisphere is used as control tissue. Tumor investigation of adjacent slices is carried out by LA-ICP-MS and, in addition, autoradiographically with a tritiated ligand (3 H-PK11195) of the peripheral benzodiazepine-receptor, which is not expressed in the brain under normal, physiological conditions but during tumor development. Ion intensities of 63 Cu 1 , 64 Zn 1 , 31 P 1 and 32 S 1 in the rat brain section (thickness: 20 mm; analyzed area 12 mm by 6 mm) containing the local tumor and control area were measured by scanning with a focused laser beam at wavelength 213 nm, diameter of laser crater 50 mm and laser power density 3 Á 10 9 W cm À2 , in a cooled laser ablation chamber coupled to a double-focusing sector field ICP-MS. The quantitative determination of element distribution in a thin slice of the rat brain tissue was carried out using matrix-matched laboratory standards. The mass spectrometric analysis yielded an inhomogeneous distribution for Cu, Zn, S and P in the analyzed rat brain sections. For Cu and Zn a deficiency in and around the tumor region in comparison with the control brain tissue of the second hemisphere was found. The detection limits for distribution analysis of Zn and Cu measured by LA-ICP-MS are in the ng g À1 range. The capability and the limits of LA-ICP-MS will be studied for the imaging of element distribution in thin cross sections of brain tissues in order to create a new diagnostic method for the borders of small-size tumors.

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“…However, when a LA system is coupled to an inductively coupled plasma mass spectrometer (ICP-MS), the ablated material is fully ionized in the argon plasma and the elements are detected by an element-specific detector (12 ). Only a few studies, however, have used this method for soft tissues (13,14 ). In our previous study we used LA coupled to inductively coupled plasma time-of-flight mass spectrometry (ICP-TOF-MS) for the quantification of trace elements in soft tissues.…”

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confidence: 99%

Two-Dimensional Mapping of Copper and Zinc in Liver Sections by Laser Ablation–Inductively Coupled Plasma Mass Spectrometry

2003

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Background: Metals are not homogeneously distributed in organ tissues. Although most mapping techniques, such as histologic staining methods, have been developed for element imaging on a subcellular level, many suffer from either low precision or poor detection limits. Therefore, small variations in elemental distribution cannot be identified. We developed a method for twodimensional mapping of trace elements to identify the influence of metabolic zonation by the liver on trace element distribution. Methods: A prepared homogeneous Certified Reference Material (CRM; LGC 7112, pig liver) was used to characterize the laser ablation-inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-MS) in terms of precision. Different isotopes for copper and zinc were monitored, and the use of carbon as an internal standard was investigated to correct for differences in ablation efficiency to identify the most precise mapping technique for liver samples. Results: For the homogeneous CRM, the reproducibility of the copper and zinc signals was ϳ3-24% depending on spot size and number of pulses. When carbon was used as an internal standard, the reproducibility was improved significantly. Line scan signals over a length of 1.5 mm were more precise [relative SD (RSD), 1.6 -6.1% for copper ( 63 Cu, 65 Cu) and zinc ( 64 Zn, 66 Zn) depending on the spot size, the scanning speed, and the element]. Thin section of sheep liver achieved precisions of 27-59% (raster scan) and 9 -47% (line scan) RSD for copper, whereas the precision for zinc was significantly better: 8 -18% (raster scan) and 4 -21% (line scan) RSD. Long line scans and two-dimensional element

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Spatial determination of elements in green leaves of oak trees (Quercus robur) by laser ablation-ICP-MS

Cited by 44 publications

References 0 publications

Laser ablation ICP-MS reveals patterns of copper differing from zinc in growth zones of cucumber roots

Laser ablation ICP-MS reveals patterns of copper differing from zinc in growth zones of cucumber roots

Copper, zinc, phosphorus and sulfur distribution in thin section of rat brain tissues measured by laser ablation inductively coupled plasma mass spectrometry: possibility for small-size tumor analysis

Two-Dimensional Mapping of Copper and Zinc in Liver Sections by Laser Ablation–Inductively Coupled Plasma Mass Spectrometry

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